Efficient construction of unmarked recombinant mycobacteria using an improved system

Journal of Microbiological Methods

Volumn 103, Issue , 2014, Pages 29-36

  Yang, Feng ^a   Tan, Yaoju ^b   Liu, Jia ^a   Liu, Tianzhou ^a,c   Wang, Bangxing ^a,d   Cao, Yuanyuan ^a,d   Qu, Yue ^e   Lithgow, Trevor ^e   Tan, Shouyong ^b   Zhang, Tianyu ^a  

^a GUANGZHOU INSTITUTES OF BIOMEDICINE AND HEALTH   (China)

^b Guangzhou Chest Hospital   (China)

^c UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^d ANHUI UNIVERSITY   (China)

^e MONASH UNIVERSITY   (Australia)

Author keywords

In frame deletion; Mycobacteria; Recombineering; Unmarked gene disruption; Xer recombinase system

Indexed keywords

ANTIBIOTIC AGENT;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM MUTANT; CLONING; CONTROLLED STUDY; DNA FLANKING REGION; GENE CASSETTE; GENE DELETION; GENETIC MANIPULATION; GENETIC RECOMBINATION; HYG GENE; MYCOBACTERIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; ALLELE; GENE EXPRESSION; GENE ORDER; GENETIC ENGINEERING; GENETICS; MYCOBACTERIUM SMEGMATIS; PLASMID; REPORTER GENE;

ALLELES; GENE DELETION; GENE EXPRESSION; GENE ORDER; GENES, REPORTER; GENETIC ENGINEERING; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; PLASMIDS; RECOMBINATION, GENETIC;

EID: 84902139271     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.05.007     Document Type: Article

References (30)

1. Agarwal N., Lamichhane G., Gupta R., Nolan S., Bishai W.R. Cyclic AMP intoxication of macrophages by a Mycobacterium tuberculosis adenylate cyclase. Nature 2009, 460:98-102.
2. Cascioferro A., Boldrin F., Serafini A., Prowedi R., Palu G., Manganelli Xer site-specific recombination, an efficient tool to introduce unmarked deletions into mycobacteria. Appl. Environ. Microbiol. 2010, 76:5312-5316.
3. Garbe T.R., Barathi J., Barnini S., Zhang Y., Abu-Zeid C., Tang D., Mukherjee R., Young D.B. Transformation of mycobacterial species using hygromycin resistance as selectable marker. Microbiology 1994, 140:133-138.
4. Garnier C., Churcher D., Harris S., Gordon V., Eiglmeier K., Gas S.C.E., Barry C.F., Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R., Devlin K., Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L., Oliver K., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton J., Squares R., Squares S., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 1998, 393:537-544.
5. Howard N.S., Gomez J.E., Ko C., Bishai W.R. Color selection with a hygromycin-resistance-based Escherichia coli-mycobacterial shuttle vector. Gene 1995, 166:181-182.
6. Jacobs W.R., Tuckman M., Bloom B.R. Introduction of foreign DNA into mycobacteria using a shuttle phasmid. Nature 1987, 327:532-535.
7. Lee M.H., Pascopella L., Jacobs W.R., Hatfull G.F. Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and Bacille Calmette-Guerin. Proc. Natl. Acad. Sci. U. S. A. 1991, 88:3111-3115.
8. Loewenberg S. India reports cases of totally drug-resistant tuberculosis. Lancet 2012, 379:205.
9. Mahenthiralingam E., Draper P., Davis E.O., Colston M.J. Cloning and sequencing of the gene which encodes the highly inducible acetamidase of Mycobacterium smegmatis. J. Gen. Microbiol. 1993, 139:575-583.
10. Malaga W., Perez E., Guilhot C. Production of unmarked mutations in mycobacteria using site-specific recombination. FEMS Microbiol. Lett. 2003, 219:261-268.
11. Manabe Y.C., Hatem C.L., Kesavan A.K., Durack J., Murphy J.R. Both Corynebacterium diphtheriae DtxR(E175K) and Mycobacterium tuberculosis IdeR(D177K) are dominant positive repressors of IdeR-regulated genes in M. tuberculosis. Infect. Immun. 2005, 73:5988-5994.
12. Morris P., Marinelli L.J., Jacobs-Sera D., Hendrix R.W., Hatfull G.F. Genomic characterization of mycobacteriophage Giles: evidence for phage acquisition of host DNA by illegitimate recombination. J. Bacteriol. 2008, 190:2172-2182.
13. Nahid P., Menzies D. Update in tuberculosis and nontuberculous mycobacterial disease 2011. Am. J. Respir. Crit. Care Med. 2012, 185:1266-1270.
14. Parish T., Stoker N.G. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement. Microbiology 2000, 146:1969-19755.
15. Parish T., Mahenthiralingam E., Draper P., Davis E.O., Colston E.O. Regulation of the inducible acetamidase gene of Mycobacterium smegmatis. Microbiology 1997, 143:2267-2276.
16. Pelicic V., Jackson M., Reyrat J.-C., Jacobs W.R., Gicquel B., Guilhot C. Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. U. S. A. 1997, 94:10955-10960.
17. Pham T.T., Jacobs-Sera D., Pedulla M.L., Hendrix R.W., Hatfull G.F. Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria. Microbiology 2007, 153:2711-2723.
18. Puech V., Guilhot C., Perez E., Tropis M., Armitige L.Y., Gicquel B., Daffe M. Evidence for a partial redundancy of the fibronectin-binding proteins for the transfer of mycoloyl residues onto the cell wall arabinogalactan termini of Mycobacterium tuberculosis. Mol. Microbiol. 2002, 44:1109-1122.
19. Saviola B., Bishai W.R. Method to integrate multiple plasmids into the mycobacterial chromosome. Nucleic Acids Res. 2004, 32:1.
20. Shenkerman Y., Elharar Y., Vishkautzana M., Gur E. Efficient and simple generation of unmarked gene deletions in Mycobacterium smegmatis. Gene 2014, 533:374-378.
21. Stratigopoulos G., Cundliffe E. Expression analysis of the tylosin-biosynthetic gene cluster: pivotal regulatory role of the thlQ product. Chem. Biol. 2002, 9:71-78.
22. Sweeney K.A., Dao D.N., Goldberg M.F., Hsu T., Venkataswamy M.M., Henao-Tamayo M., Ordway D., Sellers R.S., Jain P., Chen B., Chen M., Kim J., Lukose R., Chan J., Orme I.M., Porcelli S.A., Jacobs W.R. A recombinant Mycobacterium smegmatis induces potent bactericidal immunity against Mycobacterium tuberculosis. Nat. Med. 2011, 17:1261-1268.
23. van Kessel J.C., Hatfull G.F. Recombineering in Mycobacterium tuberculosis. Nat. Methods 2007, 4:147-152.
24. van Kessel J.C., Hatfull G.F. Mycobacterial recombineering. Methods Mol. Biol. 2008, 435:203-215.
25. van Kessel J.C., Marinelli L.J., Hatfull G.F. Recombineering mycobacteria and their phages. Nat. Rev. Microbiol. 2008, 6:851-857.
26. Zhang T., Wang L., Xu G., Chen Y., Zhang Y., Li Y. Disruption of ste23 gene affects composition profile and bioactivity of exopolysaccharide produced by Streptomyces sp. 139. Lett. Appl. Microbiol. 2006, 42:132-137.
27. Zhang T., Wang L., Xu G., Chen Y., Zhang Y., Li Y. Disruption of the ste22 gene encoding a glycosyltransferase and its function in biosynthesis of Ebosin in Streptomyces sp. 139. Curr. Microbiol. 2006, 52:55-59.
28. Zhang T., Bishai W.R., Grosset J.H., Nuermberger E.R. Rapid assessment of antibacterial activity against Mycobacterium ulcerans by using recombinant luminescent strains. Antimicrob. Agents Chemother. 2010, 54:2806-2813.
29. Zhang T., Li S.Y., Nuermberger E.L. Autoluminescent Mycobacterium tuberculosis for rapid, real-time, non-invasive assessment of drug and vaccine efficacy. PLoS One 2012, 7:e29774.
30. Zhang T., Li S.-Y., Converse P.J., Grosset J.H., Nuermberger E.L. Rapid, serial, non-invasive assessment of drug efficacy in mice with autoluminescent Mycobacterium ulcerans infection. PLoS Negl. Trop. Dis. 2013, 7:e2598.