Efficient and simple generation of unmarked gene deletions in Mycobacterium smegmatis

Gene

Volumn 533, Issue 1, 2014, Pages 374-378

  Shenkerman, Yael ^a   Elharar, Yifat ^a   Vishkautzan, Marina ^a   Gur, Eyal ^a  

^a BEN GURION UNIVERSITY OF THE NEGEV   (Israel)

Author keywords

Gene deletion; Homologous recombination; Mycobacterium; Pup

Indexed keywords

BACTERIAL PROTEIN; CHE9C PROTEIN; DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; HYGROMYCIN; KANAMYCIN; MICROORGANISM PROTEIN; PRCSBA PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENETICS; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIOPHAGE; BACTERIUM MUTANT; CHROMOSOME; CONTROLLED STUDY; GENE CASSETTE; GENE DELETION; GENE DISRUPTION; GENE EXPRESSION; GENE INSERTION; GENETIC SCREENING; HOMOLOGOUS RECOMBINATION; MYCOBACTERIUM SMEGMATIS; NONHUMAN; OPERON; PRIORITY JOURNAL;

20S; A BACILLUS SUBTILIS GENE ENCODING THE ENZYME LEVANSUCRASE; A CHE9C PROTEIN ENCODED BY THE 60 GENE; A CHE9C PROTEIN ENCODED BY THE 61 GENE; A HYGROMYCIN RESISTANCE GENE; A KANAMYCIN RESISTANCE GENE; A MYCOBACTERIAL BACTERIOPHAGE; A P1 RECOMBINASE; AN ESCHERICHIA COLI BACTERIOPHAGE; APH; CHE9C; CRE; DNA SEQUENCES RECOGNIZED BY THE CRE RECOMBINASE; DOUBLE STRANDED DNA; DSDNA; GENE DELETION; GFP; GP60; GP61; GREEN FLUORESCENCE PROTEIN; HOMOLOGOUS RECOMBINATION; HYG; LOXP SITES; MYCOBACTERIUM; P1; PAFA; PRCA; PRCB; PRCS; PROKARYOTIC UBIQUITIN LIKE PROTEIN; PROTEASOME ACCESSORY FACTOR A; PROTEASOME CATALYTIC PARTICLE; PUP; RED FLUORESCENCE PROTEIN; RFP; SACB; THE MYCOBACTERIUM SMEGMATIS GENE ENCODING PUP; THE MYCOBACTERIUM SMEGMATIS GENE ENCODING THE PROTEASOME Α SUBUNIT; THE MYCOBACTERIUM SMEGMATIS GENE ENCODING THE PROTEASOME Β SUBUNIT;

BASE SEQUENCE; BLOTTING, SOUTHERN; CHROMOSOMES, BACTERIAL; DNA PRIMERS; GENE DELETION; GENES, BACTERIAL; MYCOBACTERIUM SMEGMATIS;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; SACCHAROMYCES CEREVISIAE;

EID: 84887245884     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2013.09.082     Document Type: Article

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