Plasmid deficiency in urogenital isolates of Chlamydia trachomatis reduces infectivity and virulence in a mouse model

Pathogens and Disease

Volumn 70, Issue 1, 2014, Pages 61-69

  Sigar, Ira M ^a   Schripsema, Justin H ^a   Wang, Yibing ^b   Clarke, Ian N ^b   Cutcliffe, Lesley T ^b   Seth Smith, Helena M B ^c,g   Thomson, Nicholas R ^c   Bjartling, Carina ^d   Unemo, Magnus ^e   Persson, Kenneth ^f   Ramsey, Kyle H ^a  

^a MIDWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

^c WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

^d LUND UNIVERSITY HOSPITAL   (Sweden)

^e ÖREBRO UNIVERSITY HOSPITAL   (Sweden)

^f Malmö University Hospital   (Sweden)

^g FUNCTIONAL GENOMICS CENTER ZURICH   (Switzerland)

Author keywords

Chlamydia; Genome; Infection; Mouse; Plasmid; Virulence

Indexed keywords

CHECKPOINT KINASE 2;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY RESPONSE; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE SEQUENCE; GENOME; HUMAN; IN VIVO STUDY; MORBIDITY; MOUSE; NONHUMAN; PATHOGEN LOAD; PLASMID; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; SEROTYPE; UROGENITAL SYSTEM; UROGENITAL TRACT INFECTION; WEIGHT CHANGE; WEIGHT GAIN; WEIGHT REDUCTION;

CHLAMYDIA; GENOME; INFECTION; MOUSE; PLASMID; VIRULENCE;

ANIMALS; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; DISEASE MODELS, ANIMAL; FEMALE; GENOME, BACTERIAL; MICE; PLASMIDS; RESPIRATORY SYSTEM; UROGENITAL SYSTEM; VIRULENCE;

EID: 84901995326     PISSN: None     EISSN: 2049632X     Source Type: Journal    
DOI: 10.1111/2049-632X.12086     Document Type: Article

References (35)

1. Assefa S, Keane TM, Otto TD, Newbold C & Berriman M (2009) ABACAS: algorithm-based automatic contiguation of assembled sequences. Bioinformatics 25: 1968-1969.
2. Bjartling C, Osser S, Johnsson A & Persson K (2009) Clinical manifestations and epidemiology of the new genetic variant of Chlamydia trachomatis. Sex Transm Dis 36: 529-535.
3. Carlson JH, Whitmire WM, Crane DD et al. (2008) The Chlamydia trachomatis plasmid is a transcriptional regulator of chromosomal genes and a virulence factor. Infect Immun 76: 2273-2283.
4. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG & Parkhill J (2005) ACT: the Artemis comparison tool. Bioinformatics 21: 3422-3423.
5. Chain PS, Grafham DV, Fulton RS et al. (2009) Genomics. Genome project standards in a new era of sequencing. Science 326: 236-237.
6. Fox J, Barthold S, Newcomer C, Smith A, Quimby F & Davisson M, eds. (2007) The Mouse in Biomedical Research, Vol. 2: Diseases. Elsevier, New York, NY.
7. Frazer LC, Darville T, Chandra-Kuntal K, Andrews CW Jr, Zurenski M, Mintus M, Abdelrahman YM, Belland RJ, Ingalls RR & O'Connell CM (2012) Plasmid-cured Chlamydia caviae activates TLR2-dependent signaling and retains virulence in the guinea pig model of genital tract infection. PLoS One 7: e30747.
8. Harris SR, Clarke IN, Seth-Smith HM et al. (2012) Whole-genome analysis of diverse Chlamydia trachomatis strains identifies phylogenetic relationships masked by current clinical typing. Nat Genet 44: 413-419, S1.
9. He X, Nair A, Mekasha S, Alroy J, O'Connell CM & Ingalls RR (2011) Enhanced virulence of Chlamydia muridarum respiratory infections in the absence of TLR2 activation. PLoS One 6: e20846.
10. Kari L, Whitmire WM, Olivares-Zavaleta N et al. (2011) A live-attenuated chlamydial vaccine protects against trachoma in nonhuman primates. J Exp Med 208: 2217-2223.
11. Miyairi I, Laxton JD, Wang X, Obert CA, Arva Tatireddigari VR, van Rooijen N, Hatch TP & Byrne GI (2011) Chlamydia psittaci genetic variants differ in virulence by modulation of host immunity. J Infect Dis 204: 654-663.
12. O'Connell CM, Ingalls RR, Andrews CW Jr, Scurlock AM & Darville T (2007) Plasmid-deficient Chlamydia muridarum fail to induce immune pathology and protect against oviduct disease. J Immunol 179: 4027-4034.
13. O'Connell CM, Abdelrahman YM, Green E, Darville HK, Saira K, Smith B, Darville T, Scurlock AM, Meyer CR & Belland RJ (2011) Toll-like receptor 2 activation by Chlamydia trachomatis is plasmid dependent, and plasmid-responsive chromosomal loci are coordinately regulated in response to glucose limitation by C. trachomatis but not by C. muridarum. Infect Immun 79: 1044-1056.
14. Olivares-Zavaleta N, Whitmire W, Gardner D & Caldwell HD (2010) Immunization with the attenuated plasmidless Chlamydia trachomatis L2(25667R) strain provides partial protection in a murine model of female genitourinary tract infection. Vaccine 28: 1454-1462.
15. Persson K, Bredberg A & Bojs G (1996) Two strains of Chlamydia trachomatis without detectable 7.5 kb plasmid. Proceedings: Third Meeting of the European Society for Chlamydia Research, Vienna, Austria, September 11-14, 1996 (Stary A, Ed), pp. 33-36. Esculapio, Vienna, Austria.
16. Ramsey KH, Soderberg LSF & Rank RG (1988) Resolution of chlamydial genital infection in B-cell-deficient mice and immunity to reinfection. Infect Immun 56: 1320-1325.
17. Ramsey KH, Newhall WJ & Rank RG (1989) Humoral immune response to chlamydial genital infection of mice with the agent of mouse pneumonitis. Infect Immun 57: 2441-2446.
18. Ramsey KH, Cotter TW, Salyer RD, Miranpuri GS, Yanez MA, Poulsen CE, DeWolfe JL & Byrne GI (1999) Prior genital tract infection with a murine or human biovar of Chlamydia trachomatis protects mice against heterotypic challenge infection. Infect Immun 67: 3019-3025.
19. Ramsey KH, Sigar IM, Schripsema JH, Denman CJ, Bowlin AK, Myers GA & Rank RG (2009) Strain and virulence diversity in the mouse pathogen Chlamydia muridarum. Infect Immun 77: 3284-3293.
20. Rank RG. (2007) Chlamydial diseases. The Mouse in Biomedical Research (Fox J, Barthold S, Newcomer C, Smith A, Quimby F & Davisson M, eds). Academic Press, New York, NY.
21. Reed LJ & Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Epidemiol 27: 493-497.
22. Ripa T & Nilsson P (2006) A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill 11: E061109.
23. Russell M, Darville T, Chandra-Kuntal K, Smith B, Andrews CW Jr & O'Connell CM (2011) Infectivity acts as in vivo selection for maintenance of the chlamydial cryptic plasmid. Infect Immun 79: 98-107.
24. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream MA & Barrell B (2000) Artemis: sequence visualization and annotation. Bioinformatics 16: 944-945.
25. Seth-Smith HM, Harris SR, Persson K et al. (2009) Co-evolution of genomes and plasmids within Chlamydia trachomatis and the emergence in Sweden of a new variant strain. BMC Genomics 10: 239.
26. Song L, Carlson JH, Whitmire WM et al. (2013) Chlamydia trachomatis plasmid-encoded Pgp4 is a transcriptional regulator of virulence-associated genes. Infect Immun 81: 636-644.
27. Thomas NS, Lusher M, Storey CC & Clarke IN (1997) Plasmid diversity in Chlamydia Microbiol 143(Pt 6): 1847-1854.
28. Tuffrey M, Falder P, Gale J, Quinn R & Taylor-Robinson D (1986a) Infertility in mice infected genitally with a human strain of Chlamydia trachomatis. J Reprod Fert 78: 251-260.
29. Tuffrey M, Falder T, Gale J & Taylor-Robinson D (1986b) Salpingitis in mice induced by human strains of Chlamydia trachomatis. Br J Exp Pathol 67: 605-616.
30. Ulland TK, Buchan BW, Ketterer MR, Fernandes-Alnemri T, Meyerholz DK, Apicella MA, Alnemri ES, Jones BD, Nauseef WM & Sutterwala FS (2010) Cutting edge: mutation of Francisella tularensis mviN leads to increased macrophage absent in melanoma 2 inflammasome activation and a loss of virulence. J Immunol 185: 2670-2674.
31. Unemo M & Clarke IN (2011) The Swedish new variant of Chlamydia trachomatis. Curr Opin Infect Dis 24: 62-69.
32. Unemo M, Seth-Smith HM, Cutcliffe LT et al. (2010) The Swedish new variant of Chlamydia trachomatis: genome sequence, morphology, cell tropism and phenotypic characterization. Microbiol 156: 1394-1404.
33. Wang Y, Kahane S, Cutcliffe LT, Skilton RJ, Lambden PR & Clarke IN (2011) Development of a transformation system for Chlamydia trachomatis: restoration of glycogen biosynthesis by acquisition of a plasmid shuttle vector. PLoS Pathog 7: e1002258.
34. Wang Y, Cutcliffe LT, Skilton RJ, Persson K, Bjartling C & Clarke IN (2012) Genetic transformation of a naturally-occurring plasmid-free genital tract isolate of Chlamydia trachomatis (serovar F). Proceedings: Seventh Meeting of the European Society for Chlamydia Research, Vienna, Austria (Stary A, Ed), pp. 213-216. Esculapio, Vienna, Austria.
35. Zerbino DR & Birney E (2008) Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res 18: 821-829.