Molecular detection of virulence factors among food and clinical Enterococcus faecalis strains in South Brazil

Brazilian Journal of Microbiology

Volumn 45, Issue 1, 2014, Pages 327-332

  Medeiros, A W ^a   Pereira, R I ^a   Oliveira, D V ^a   Martins, P D ^a   d'Azevedo, P A ^b   Van der Sand, S ^a   Frazzon, J ^c   Frazzon, A P G ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b UNIVERSIDADE FEDERAL DE CIÊNCIAS DA SAÚDE DE PORTO ALEGRE   (Brazil)

^c FEDERAL UNIVERSITY OF RIO GRANDE   (Brazil)

Author keywords

Biofilm formation; Clinical enterococci; Food enterococci; Virulence determinants

Indexed keywords

BACTERIAL PROTEIN; GELATINASE; VIRULENCE FACTOR;

BIOFILM; BRAZIL; COMPARATIVE STUDY; ENTEROCOCCUS FAECALIS; FOOD CONTROL; GENETICS; GRAM POSITIVE INFECTION; GROWTH, DEVELOPMENT AND AGING; HEMOLYSIS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGY; PHYSIOLOGY;

BACTERIAL PROTEINS; BIOFILMS; BRAZIL; ENTEROCOCCUS FAECALIS; FOOD MICROBIOLOGY; GELATINASES; GRAM-POSITIVE BACTERIAL INFECTIONS; HEMOLYSIS; HUMANS; VIRULENCE FACTORS;

EID: 84901929840     PISSN: 15178382     EISSN: 16784405     Source Type: Journal    
DOI: 10.1590/S1517-83822014005000031     Document Type: Article

References (41)

1. Abriouel H. Omar NB, Molinos AC, López RL, Grande MJ, Martínez-Viedma P., Ortega E, Cañamero MM, Galvez A (2008) Comparative analysis of genetic diversity and incidence of virulence factors and antibiotic resistance among enterococcal populations from raw fruit and vegetable foods, water and soil, and clinical samples. Int J Food Microbiol 123:38-49.
2. Baldassarri L., Cecchini R, Bertuccini L, Ammendolia MG, Iosi F, Arciola C. R, Montanaro L, Rosa RD, Gherardi G, Dicuonzo G (2001) Enterococcus spp. produces slime and survives in rat peritoneal macrophages. Med Microbiol Immunol 190:113-120.
3. Bittencourt E., Suzart S (2004) Occurrence of virulence-associated genes in clinical Enterococcus faecalis strains isolated in Londrina. Brazil J Med Microbiol 53:1069-1073.
4. Cariolato D., Andrighetto C, Lombardi A (2008) Occurrence of virulence factors and antibiotic resistances in Enterococcus faecalis and Enterococcus faecium collected from dairy and human samples in North Italy. Food Control 19:886-892.
5. Christensen G., Simpson WA, Younger JJ, Baddour LM, Barrett FF, Melton DM, Beachey EH (1985) Adherence of coagulase-negative staphylococci to plastic tissue culture plates: A quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol 22:996-1006.
6. Cosentino S., Podda GS, Corda A, Fadda ME, Deplano M, Pisano MB (2010) Molecular detection of virulence factors and antibiotic resistance pattern in clinical Enterococcus faecalis strains in Sardinia. J Prev Med Hyg 51:31-36.
7. Chuang-Smith O. N, Wells CL, Henry-Stanley MJ, Dunny GM (2010) Acceleration of Enterococcus faecalis biofilm formation by aggregation substance expression in an ex vivo model of cardiac valve colonization. PLoS ONE 5:e15798.
8. d'Azevedo PA, Dias CAG, Teixeira LM (2006) Genetic diversity and antimicrobial resistance of enterococcal isolates from southern region of Brazil. Rev Inst Med Trop 48:11-16.
9. Drahovská H., Slobodníková L, Kocíncová D, Seman M, Konceková R, Trupl J, Turna J (2004) Antibiotic resistance and virulence factors among clinical and food enterococci isolated in Slovakia. Folia Microbiol 49:763-768.
10. Dupont H., Vael C, Muller-Serieys C, Chosidow D, Mantz J, Marmuse J. P, Andremont A, Goossens H, Desmonts JM (2008) Prospective evaluation of virulence factors of enterococci isolated from patients with peritonitis: Impact on outcome. Diag Microbiol Infect Dis 60:247-253.
11. Duprè I., Zanetti S, Schito AM, Fadda G, Sechi LA (2003) Incidence of virulence determinants in clinical Enterococcus faecium and Enterococcus faecalis isolates collected in Sardinia (Italy). J Med Microbiol 52:491-498.
12. Dutka-Mahlen S., Evers S, Courvalin P (1995) Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. J Clin Microbiol 33:24-27.
13. Eaton T. J, Gasson MJ (2001) Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 67:1628-1635.
14. Facklam R. R, Carvalho MGS, Teixeira LM (2002) History, taxonomy, biochemical characteristics, and antibiotic susceptibility testing of enterococci. In: Gilmore MS, Clewel DB, Courvalin P., Dunny GM, Murray BE, Rice, L.B.(eds). The enterococci: pathogenesis, molecular biology, and antibiotic resistance. ASM Press, Washington, USA, pp. 1-54.
15. Fischer K., Phillips C (2009) The ecology, epidemiology and virulence of Enterococcus. Microbiology 155:1749-1757.
16. Franz C. MAP, Stiles ME, Schleifer KH, Holzapfel WH (2003) Enterococci in foods-a conundrum for food safety. Int J Food Microbiol 88:105-122.
17. Frazzon A. PG, Gama BA, Hermes V, Bierhals CG, Pereira RI, Guedes A. G, d'Azevedo PA, Frazzon J (2009) Prevalence of antimicrobial resistance and molecular characterization of tetracycline resistance mediated by tet(M) and tet(L) genes in Enterococcus spp. isolated from food in Southern Brazil. World J Microbiol Biotechnol 26:365-370.
18. Gaspar F. B, Crespo MTB, Lopes MFS (2009) Proposal for a reliable enterococcal cytolysin production assay avoiding apparent incongruence between phenotype and genotype. J Med Microbiol 58:1122-1124.
19. Giraffa G. (2006) Enterococci in foods. FEMS Microbiol Rev 26:163-171.
20. Hancock L. E, Perego M (2004) The Enterococcus faecalis fsr two-component system controls biofilm development trough production of gelatinase. J Bacteriol 186:5629-5639.
21. Hufnagel M., Koch S, Creti R, Baldassarri L, Huebner J (2004) A putative sugar-binding transcriptional regulator in a novel gene locus in Enterococcus faecalis contributes to production of biofilm and prolonged bacteremia in mice. J Infect Dis 189:420-430.
22. Huycke M. M, Sahm DF, Gilmore MS. (1998) Multiple-drug resistant enterococci: the nature of the problem and an agenda for the future. Emerging Infect Dis 4:239-249.
23. Kayser F. H (2003) Safety aspects of enterococci from the medical point of view. Int Food Microbial 88:255-262.
24. Koch S., Hufnagel M, Theilacker C, Huebner J (2004) Enterococcal infections: host response, therapeutic and prophylactic possibilities. Vaccine 22:822-830.
25. Lebreton F., Riboulet-Bisson E, Serror P, Sanguinete M, Posteraro B, Torelli R, Hartke A, Auffray Y, Giard JC (2009) Ace, which encodes an adhesin in Enterococcus faecalis, is regulated by Ers and is involved in virulence. Infect Immun 77:2832-2839.
26. Lopes M. FS, Simões AN, Tenreiro R, Marques JJ, Crespo MTB (2006) Activity and expression of a virulence factor, gelatinase, in dairy enterococci. Int J Food Microbiol 112:208-214.
27. Mannu L., Paba A, Daga E, Comunian R, Duprè I, Zanetti S, Sechi, LA (2003) Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbial 88:291-304.
28. Marinho A. R, Martins PD, Ditmer EM, d'Azevedo PA, Frazzon J, Van Der Sand ST, Frazzon, APG (2013) Biofilm formation on polystyrene under different temperatures by antibiotic resistant Enterococcus faecalis and Enterococcus faecium isolated from food Braz J Microbiol 44:423-426.
29. Marra A., Dib-Hajj F, Lamb L, Kaczmarek F, Shang W, Beckius G, Milici AJ, Medina I, Gootz TD (2007) Enterococcal virulence determinants may be involved in resistance to clinical therapy. Diagn Microbiol 58:59-65.
30. Mohamed J. A, Huang W, Nallapareddy SR, Teng F, Murray BE (2004) Influence of origin of isolates, especially endocarditis isolates, and various genes on biofilm formation by Enterococcus faecalis. Infec Immun 72:3658-3663.
31. Riboldi G. P, Frazzon J, d'Azevedo PA, Frazzon APG (2009) Antimicrobial resistance profile of Enterococcus spp. isolated from food in Southern Brazil. Braz J Microbiol 40:125-128.
32. Rosa R. D, Creti R, Venditti M, D'Amelio R, Arciola CR, Montanaro L, Baldassari, L (2006) Relationship between biofilm formation, the enterococcal surface protein (Esp) and gelatinase in clinical isolates of Enterococcus faecalis and Enterococcus faecium. FEMS Microbiol Lett 256:145-150.
33. Samuelsson A., Jonasson J, Monstein HJ, Berg S, Isaksson B (2003) Clustering of enterococcal infections in a general intensive care unit. J Hospital Infect 54:188-195.
34. Sava I. G, Heinkens E, Kropec A, Theilacker C, Willems R, Huebner J. (2010) Enterococcal surface protein contributes to persistence in the host but is not a target of opsonic and protective antibodies in Enterococcus faecium infection. J Med Microbiol 59:1001-1004.
35. Semedo T., Santos MA, Martins P, Lopes MFS, Marques JJF, Tenreiro R., Crespo MTB (2003) Comparative study using type strains and clinical and food isolates to examine hemolytic activity and occurrence of the cyl operon in enterococci. J Clin Microbiol 41:2569-2576.
36. Shankar N., Lockatell CV, Baghdayan AS, Drachenberg C, Gilmore MS, Johnson DE (2001) Role of Enterococcus faecalis surface protein Esp in the pathogenesis of ascending urinary tract infection. Infect Immun 69:4366-4372.
37. Singh K. V, Nallapareddy SR, Sillanpää J, Murray BE (2010) Importance of the collagen adhesin Ace in pathogenesis and protection against Enterococcus faecalis experimental endocarditis. PLoS Pathog 6:1-13.
38. Stepanovic S., Vukovic D, Dakic I, Savic B, Svabic-Vlahovic MA (2000) Modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 40:175-179.
39. Upadhyaya P. M, Ravikumar KL, Umapathy BL (2009) Review of virulence factors of enterococci: an emerging nosocomial pathogen. Indian J Med Microbiol 27:301-305.
40. Vogel L., Sloos JH, Spaargaren J, Suiker I, Dijkshoorn L (2000) Biofilm production by Staphylococcus epidermidis isolates associated with catheter related bacteremia. Diagn Microbiol Infect Dis 36:139-141.
41. Waters C. M, Antiporta MH, Murray BE, Dunny GM (2003) Role of the Enterococcus faecalis GelE protease in determination of cellular chain length, supernatant pheromone levels, and degradation of fibrin and misfolded surface proteins. J Bacteriol 185:3613-3623.