Small footprint continuous flow PCR devices for a 96-well CFPCR multi-reactor platform

Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop

Volumn , Issue , 2008, Pages 114-117

  Park, D S ^a   Chen, P C ^a   You, B H ^a   Kim, N ^a   Park, T ^a   Lee, T Y ^a   Datta, P ^a   Desta, Y ^b   Soper, S A ^a   Nikitopoulos, D E ^a   Murphy, M C ^a  

^a LOUISIANA STATE UNIVERSITY   (United States)

^b Biofluidica Microtechnologies   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTUATORS; MICROSYSTEMS; SOLID-STATE SENSORS; TEMPERATURE CONTROL;

CONTINUOUS FLOWS; DNA-TEMPLATE; HIGH THROUGHPUT; HIGHLY PARALLELS; MULTI-REACTORS; SMALL FOOTPRINTS; STEADY-STATE TEMPERATURE; THERMAL ISOLATION;

POLYMERASE CHAIN REACTION;

EID: 84901797715     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.31438/trf.hh2008.32     Document Type: Conference Paper

References (13)

1. T. K. Christopoulos, 1999, “Nucleic Acid Analysis”, Anal. Chem., 71, pp. 425R-438R (1999).
2. P. J. Obeid, T. K. Christopoulos, H. J. Crabtree, and C. J. Backhouse, “Microfabricated Device for DNA and RNA Amplification by Continuous-Flow Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction with Cycle Number Selection”, Anal. Chem., 75, pp. 288-295 (2003).
3. C. Zang and D. Xing, “Miniaturized PCR Chips for Nucleic Acid Amplification and Analysis: Latest Advances and Future Trends”, Nucleic Acids Research, 35(13), pp. 4223-4237 (2007).
4. M. A. Northrup, M. T. Chang, R. M. White, and R. T. Watson, “DNA Amplification in a Microfabricated Reaction Chamber”, Proceedings of the 7th International Conference on Solid-State Sensors and Actuators (Transducers ’93), Yokohama, Japan, 6/ 07-10/93, pp. 924-926.
5. C. J. Easley, J. M. Karlinsey, and J. P. Landers, “On-Chip Pressure Injection for Integration of Infrared-Mediated DNA Amplification with Electrophoretic Separation”, Lab Chip, 6, pp. 601-610 (2006).
6. M. U. Kopp, A. J. deMello, and A. Manz, “Chemical Amplification: Continuous-Flow PCR on a Chip”, Science, 280, pp. 1046-1048 (1998).
7. M. Hashimoto, P.-C. Chen, M. W. Mitchell, D. E. Nikitopoulos, S. A. Soper, and M. C., Murphy, “Rapid PCR in a Continuous Flow Device”, Lab Chip, 4, pp. 638-645 (2004).
8. C. N. Liu, N. M. Toriello, and R. A. Mathies, “Multichannel PCR-CE Microdevice for Genetic Analysis”, Anal. Chem., 78, pp. 5474-5479 (2006).
9. Z.-Q. Zou, X. Chen, Q.-H. Jin, M.-S. Yang, and J.-L. Zhao, “A Novel Miniaturized PCR Multi-Reactor Array Fabricated Using Flip-Chip Bonding Techniques”, J. Micromech. Microeng., 15, pp.1476-1481 (2005).
10. Microplate Standards Development Committee (2004) For Microplates – Footprint Dimensions – ANSI/SBS 1-2004, eds. Secretariat Society for Biomolecular Screening. Danbury, CT.
11. D. S. Park, P.-C. Chen, B. H. You, N. Kim, T. Park, P. Datta, Y. Desta, S. A. Soper, D. E. Nikitopoulos, and M. C. Murphy, “Optimization of Geometry for Continuous Flow PCR Devices in a Titer Plate-Based PCR Multi-Reactor Platform”, Proc. ASME IMECE 2007, IMECE2007-42135.
12. P.-C. Chen, D. E. Nikitopoulos, S. A. Soper, and M. C. Murphy, “Temperature Distribution Effects on Micro-CFPCR Performance”, Biomedical Microdevices, 10(2), pp. 141-152 (2008).
13. D. S.-W. Park, M. L. Hupert, M. A. Witek, B. H. You, P. Datta, J. Guy, J.-B. Lee, S. A. Soper, D. E. Nikitopoulos, and M. C. Murphy, “A Titer Plate-Based Polymer Microfluidic Platform for High Throughput Nucleic Acid Purification”, Biomedical Microdevices, 10(1), pp. 21-33 (2008).