Deformability cytometry of embryonic stem cells reveals consistent mechanical properties across cell lines

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Volumn , Issue , 2012, Pages 974-976

  Masaeli, Mahdokht ^a,b   Tse, Henry T K ^a,b   Gossett, Daniel R ^a,b   Clark, Amander T ^c,d   Di Carlo, Dino ^a,b  

^a Engineering IV   (United States)

^b CALIFORNIA NANOSYSTEMS INSTITUTE   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research   (United States)

Author keywords

Label free assay; Microfluidics; Pluripotency; Stem cell

Indexed keywords

ASSAYS; ATOMIC FORCE MICROSCOPY; CELL CULTURE; DEFORMATION; DIAGNOSIS; MAMMALS; MECHANICAL PROPERTIES; MICROFLUIDICS; QUALITY CONTROL; STEM CELLS;

EMBRYONIC STEM CELLS; HIGH THROUGHPUT; LABEL-FREE ASSAYS; MICROPIPETTE ASPIRATION; MOUSE EMBRYONIC STEM CELLS; PLURIPOTENCY; QUALITY CONTROL STRATEGIES; REGENERATIVE MEDICINE;

BIOMECHANICS;

EID: 84901770755     PISSN: None     EISSN: None     Source Type: Conference Proceeding    
DOI: None     Document Type: Conference Paper

References (6)

1. Knoepfler, P.S., Deconstructing stem cell tumorigenicity: A roadmap to safe regenerative medicine. Stem Cells, 2009. 27(5): p. 1050-6.
2. Tse, H.T.K., et al., Strategies for Implementing Hardware-Assisted High-Throughput Cellular Image Analysis. Journal of the Association for Laboratory Automation, 2011. 16(6): p. 422-430.
3. Pajerowski, J.D., et al., Physical plasticity of the nucleus in stem cell differentiation. Proc Natl Acad Sci U S A, 2007. 104(40): p. 15619-24.
4. Dahl, K.N., A.J. Ribeiro, and J. Lammerding, Nuclear shape, mechanics, and mechanotransduction. Circ Res, 2008. 102(11): p. 1307-18.
5. Gossett, D.R., et al., Hydrodynamic stretching of single cells for large population mechanical phenotyping. Proc Natl Acad Sci U S A, 2012. 109(20): p. 7630-5.
6. Di Carlo, D., et al., Continuous inertial focusing, ordering, and separation of particles in microchannels. Proc Natl Acad Sci U S A, 2007. 104(48): p. 18892-7.