Engineered human lipocalin as an antibody mimetic: Application to analysis of the small peptide hormone hepcidin

Clinical Chemistry

Volumn 60, Issue 6, 2014, Pages 897-899

  Grebenchtchikov, Nicolai ^a   Geurts Moespot, Anneke J ^a   Trentmann, Stefan ^b   Andersen, Nicole ^b   Bel Aiba, Rachida S ^b   Allersdorfer, Andrea ^b   Laarakkers, Coby M ^a   Sweep, Fred C ^a   Tjalsma, Harold ^a   Hohlbaum, Andreas M ^b   Swinkels, Dorine W ^a  

^a RADBOUD UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Pieris Pharmaceuticals GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; BIOLOGICAL MARKER; HEPCIDIN; LIPOCALIN; MONOCLONAL ANTIBODY; PEPTIDE HORMONE;

BACTERIAL CELL; BINDING AFFINITY; BIOENGINEERING; HUMAN; IRON METABOLISM; LETTER; LIMIT OF DETECTION; PROTEIN ANALYSIS; PROTEIN TARGETING;

ANTIBODIES, MONOCLONAL; HEPCIDINS; HUMANS; LIPOCALINS; MOLECULAR MIMICRY; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

EID: 84901748611     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2014.221671     Document Type: Letter

References (5)

1. Schönfeld D, Matschiner G, Chatwell L, Trentmann S, Gille H, Hülsmeyer M, et al. An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies. Proc Natl Acad Sci USA 2009;106:8198-203.
2. Kroot JJ, Tjalsma H, Fleming RE, Swinkels DW. Hepcidin in human iron disorders: diagnostic implications. Clin Chem 2011;257:1650-69.
3. Hohlbaum AM, Trentman S, Gille H, Allersdorfer A, Belaiba RS, Huelsmeyer M, et al. Discovery and preclinical characterization of a novel hepcidin antagonist with tunable PK/PD properties for the treatment of anemia in different patient populations (Abstract). Blood 2011;118:687.
4. Laarakkers CM, Wiegerinck ET, Klaver S, Kolodziejczyk M, Gille H, Hohlbaum AM, et al. Improved mass spectrometry assay for plasma hepcidin: detection and characterization of a novel hepcidin isoform. PLoS One 2013;8:e75518.
5. Kroot JJ, Laarakkers CM, Geurts-Moespot AJ, Grebenchtchikov N, Pickkers P, van Ede AE, et al. Immunochemical and mass-spectrometry-based serum hepcidin assays for iron metabolism disorders. Clin Chem 2010;56:1570-9.