High-level soluble expression of the hemA gene from Rhodobacter capsulatus and comparative study of its enzymatic properties

Journal of Zhejiang University: Science B

Volumn 15, Issue 5, 2014, Pages 491-499

  Lou, Jia Wei ^a   Zhu, Li ^a   Wu, Mian Bin ^a   Yang, Li Rong ^a   Lin, Jian Ping ^a   Cen, Pei Lin ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

5 Aminolevulinic acid; Enzymatic properties; High level expression; Rhodobacter capsulatus

Indexed keywords

5 AMINOLEVULINATE SYNTHASE; AMINOLEVULINIC ACID; BARIUM ION; CALCIUM ION; COBALT; COPPER ION; DODECYL SULFATE SODIUM; EDETIC ACID; MANGANESE; POTASSIUM ION; RECOMBINANT PROTEIN; SUCCINYL COENZYME A; ZINC ION; ALDEHYDE DEHYDROGENASE; GLUTAMYL TRNA REDUCTASE;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STABILITY; EXPRESSION VECTOR; FED BATCH FERMENTATION; GENE EXPRESSION; GENETIC RECOMBINATION; HEMA GENE; MICHAELIS CONSTANT; MOLECULAR CLONING; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RHODOBACTER CAPSULATUS; RHODOBACTER SPHAEROIDES; TEMPERATURE; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PHYSIOLOGY; PROCEDURES; PROTEIN ENGINEERING; SOLUBILITY;

5-AMINOLEVULINATE SYNTHETASE; ALDEHYDE OXIDOREDUCTASES; AMINOLEVULINIC ACID; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RHODOBACTER CAPSULATUS; SOLUBILITY; SUBSTRATE SPECIFICITY;

EID: 84901239965     PISSN: 16731581     EISSN: 18621783     Source Type: Journal    
DOI: 10.1631/jzus.B1300283     Document Type: Article

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