A pulse-chase epitope labeling to study cellular dynamics of newly synthesized proteins: A novel strategy to characterize NPC biogenesis and ribosome maturation/export

Methods in Cell Biology

Volumn 122, Issue , 2014, Pages 147-163

  Stelter, Philipp ^a   Hurt, Ed ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Epitope pulse chase labeling; Immunofluorescence; Immunoprecipitation; NPC; Nuclear cytoplasmic fractionation; Posttranslational modifications; Ribosomes; Yeast

Indexed keywords

EPITOPE; MESSENGER RNA; METIROSINE; MULTIPROTEIN COMPLEX; NUCLEOPORIN; O-METHYLTYROSINE; RIBOSWITCH; SACCHAROMYCES CEREVISIAE PROTEIN; STOP CODON;

BIOSYNTHESIS; CHEMISTRY; CYTOLOGY; FLUORESCENT ANTIBODY TECHNIQUE; GENETICS; IMMUNOPRECIPITATION; LARGE RIBOSOMAL SUBUNIT; METABOLISM; NUCLEOTIDE SEQUENCE; PROTEIN PROCESSING; RIBOSWITCH; SACCHAROMYCES CEREVISIAE; STAINING; STOP CODON;

BASE SEQUENCE; CODON, TERMINATOR; EPITOPES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; IMMUNOPRECIPITATION; METHYLTYROSINES; MULTIPROTEIN COMPLEXES; NUCLEAR PORE COMPLEX PROTEINS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RIBOSOME SUBUNITS, LARGE, EUKARYOTIC; RIBOSWITCH; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; STAINING AND LABELING;

EID: 84900865477     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-417160-2.00007-2     Document Type: Chapter

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