Human erythrocytes transport dehydroascorbic acid and sugars using the same transporter complex

American Journal of Physiology - Cell Physiology

Volumn 306, Issue 10, 2014, Pages

  Sage, Jay M ^a   Carruthers, Anthony ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Dehydroascorbic acid transport; Erythrocyte; Glucose transport; GLUT1

Indexed keywords

3 METHYLGLUCOSE; CARBOHYDRATE; DEHYDROASCORBIC ACID; GLUCOSE TRANSPORTER 1;

ARTICLE; CELL KINETICS; CELL TRANSPORT; CONTROLLED STUDY; ERYTHROCYTE; GLUCOSE TRANSPORT; HUMAN; HUMAN CELL; MEMBRANE VESICLE; NUCLEOCYTOPLASMIC TRANSPORT; PRIORITY JOURNAL; STEADY STATE;

DEHYDROASCORBIC ACID TRANSPORT; ERYTHROCYTE; GLUCOSE TRANSPORT; GLUT1;

3-O-METHYLGLUCOSE; BINDING SITES; BINDING, COMPETITIVE; BIOLOGICAL TRANSPORT; CARBON RADIOISOTOPES; DEHYDROASCORBIC ACID; ERYTHROCYTE MEMBRANE; GLUCOSE; GLUCOSE TRANSPORTER TYPE 1; HUMANS; KINETICS; PROTEIN BINDING; TRITIUM;

EID: 84900540215     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00044.2014     Document Type: Article

References (34)

1. Baker GF, Naftalin RJ. Evidence of multiple operational affinities for D-glucose inside the human erythrocyte membrane. Biochim Biophys Acta 550: 474-484, 1979.
2. Baldwin SA, Baldwin JM, Gorga FR, Lienhard GE. Purification of the cytochalasin B binding component of the human erythrocyte monosaccharide transport system. Biochim Biophys Acta 552: 183-188, 1979.
3. Bemeur C, Ste-Marie L, Desjardins P, Vachon L, Butterworth RF, Hazell AS, Montgomery J. Dehydroascorbic acid normalizes several markers of oxidative stress and inflammation in acute hyperglycemic focal cerebral ischemia in the rat. Neurochem Int 46: 399-407, 2005.
4. Carruthers A, Melchior DL. Asymmetric or symmetric? Cytosolic modulation of human erythrocyte hexose transfer. Biochim Biophys Acta 728: 254-266, 1983.
5. Carruthers A. Facilitated diffusion of glucose. Physiol Rev 70: 1135-1176, 1990.
6. Coderre PE, Cloherty EK, Zottola RJ, Carruthers A. Rapid substrate translocation by the multisubunit, erythroid glucose transporter requires subunit associations but not cooperative ligand binding. Biochemistry 34: 9762-9773, 1995.
7. Ducruet AF, Mack WJ, Mocco J, Hoh DJ, Coon AL, D'Ambrosio AL, Winfree CJ, Pinsky DJ, Connolly ES. Preclinical evaluation of postischemic dehydroascorbic acid administration in a large-animal stroke model. Transl Stroke Res 2: 399-403, 2011.
8. Ha MN, Graham FL, D'Souza CK, Muller WJ, Igdoura SA, Schellhorn HE. Functional rescue of vitamin C synthesis deficiency in human cells using adenoviral-based expression of murine L-gulono--lactone oxidase. Genomics 83: 482-492, 2004.
9. Hamill S, Cloherty EK, Carruthers A. The human erythrocyte sugar transporter presents two sugar import sites. Biochemistry 38: 16974-16983, 1999.
10. Harrison FE, May JM. Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2. Free Radic Biol Med 46: 719-730, 2009.
11. Helgerson AL, Carruthers A. Equilibrium ligand binding to the human erythrocyte sugar transporter. Evidence for two sugar-binding sites per carrier. J Biol Chem 262: 5464-5475, 1987.
12. Huang J, Agus DB, Winfree CJ, Kiss S, Mack WJ, McTaggart RA, Choudhri TF, Kim LJ, Mocco J, Pinsky DJ, Fox WD, Israel RJ, Boyd TA, Golde DW, Connolly ES. Dehydroascorbic acid, a blood-brain barrier transportable form of vitamin C, mediates potent cerebroprotection in experimental stroke. Proc Natl Acad Sci USA 98: 11720-11724, 2001.
13. Leitch JM, Carruthers A. ATP-dependent sugar transport complexity in human erythrocytes. Am J Physiol Cell Physiol 292: C974-C986, 2007.
14. Leitch JM, Carruthers A. α-and β-Monosaccharide transport in human erythrocytes. Am J Physiol Cell Physiol 296: C151-C161, 2009.
15. Lieb WR, Stein WD. Testing and characterizing the simple carrier. Biochim Biophys Acta 373: 178-196, 1974.
16. Lowe AG, Walmsley AR. The kinetics of glucose transport in human red blood cells. Biochim Biophys Acta 857: 146-154, 1986.
17. Mack WJ, Mocco J, Ducruet AF, Laufer I, King RG, Zhang Y, Guo W, Pinsky DJ, Connolly ES. A cerebroprotective dose of intravenous citrate/sorbitol-stabilized dehydroascorbic acid is correlated with increased cerebral ascorbic acid and inhibited lipid peroxidation after murine reperfused stroke. Neurosurgery 59: 383-388, 2006.
18. Mann GV, Newton P. The membrane transport of ascorbic acid. Ann NY Acad Sci 258: 243-252, 1975.
19. May JM. Vitamin C transport and its role in the central nervous system. Subcell Biochem 56: 85-103, 2012.
20. May JM, Qu ZC, Nazarewicz R, Dikalov S. Ascorbic acid efficiently enhances neuronal synthesis of norepinephrine from dopamine. Brain Res Bull 90: 35-42, 2013.
21. May JM, Qu ZC, Whitesell RR, Cobb CE. Ascorbic acid recycling enhances the antioxidant reserve of human erythrocytes. Biochemistry 34: 12721-12728, 1995.
22. May JM, Qu ZC, Whitesell RR, Cobb CE. Ascorbate recycling in human erythrocytes: role of GSH in reducing dehydroascorbate. Free Radic Biol Med 20: 543-551, 1996.
23. Mendiratta S, Qu ZC, May JM. Erythrocyte ascorbate recycling: antioxidant effects in blood. Free Radic Biol Med 24: 789-797, 1998.
24. Miller DM. The kinetics of selective biological transport. II. Equations for induced uphill transport of sugars in human erythrocytes. Biophys J 5: 417-423, 1965.
25. Montel-Hagen A, Kinet S, Manel N, Mongellaz C, Prohaska R, Battini JL, Delaunay J, Sitbon M, Taylor N. Erythrocyte Glut1 triggers dehydroascorbic acid uptake in mammals unable to synthesize vitamin C. Cell 132: 1039-1048, 2008.
26. Montel-Hagen A, Sitbon M, Taylor N. Erythroid glucose transporters. Curr Opin Hematol 16: 165-172, 2009.
27. Puls I, Becker G, Maurer M, Mullges W. Cerebral arteriovenous transit time (CTT): a sonographic assessment of cerebral microcirculation using ultrasound contrast agents. Ultrasound Med Biol 25: 503-507, 1999.
28. Rice ME. Ascorbate regulation and its neuroprotective role in the brain. Trends Neurosci 23: 209-216, 2000.
29. Rumsey SC, Kwon O, Xu GW, Burant CF, Simpson I, Levine M. Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid. J Biol Chem 272: 18982-18989, 1997.
30. Sogin DC, Hinkle PC, Singer SJ. Characterization of the glucose transporter from human erythrocytes. J Supramol Struct 8: 447-453, 1978.
31. Steck TL, Weinstein RS, Straus JH, Wallach DF. Inside-out red cell membrane vesicles: preparation and purification. Science 168: 255-257, 1970.
32. Stein WD. Transport and Diffusion Across Cell Membranes. New York: Academic, 1986.
33. Zhang JZ, Abbud W, Prohaska R, Ismail-Beigi F. Overexpression of stomatin depresses GLUT-1 glucose transporter activity. Am J Physiol Cell Physiol 280: C1277-C1283, 2001.
34. Zoccoli MA, Baldwin SA, Lienhard GE. The monosaccharide transport system of the human erythrocyte. Solubilization and characterization on the basis of cytochalasin B binding. J Biol Chem 253: 6923-6930, 1978.