Molecular analysis of ciprofloxacin resistance mechanisms in Malaysian ESBL-producing Klebsiella pneumoniae isolates and development of mismatch amplification mutation assays (MAMA) for rapid detection of gyrA and parC mutations

BioMed Research International

Volumn 2014, Issue , 2014, Pages

  Al Marzooq, Farah ^a   Mohd Yusof, Mohd Yasim ^a   Tay, Sun Tee ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

CIPROFLOXACIN; QUINOLONE; ANTIINFECTIVE AGENT; BETA LACTAMASE; DNA TOPOISOMERASE (ATP HYDROLYSING); DNA TOPOISOMERASE IV;

AAC(6') IB CR GENE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL MUTATION; BACTERIUM ISOLATE; EXTENDED SPECTRUM BETA LACTAMASE PRODUCING KLEBSIELLA PNEUMONIAE; GENE AMPLIFICATION; GENE CLUSTER; GYRA GENE; HOSPITAL INFECTION; INFECTION CONTROL; MALAYSIA; MINIMUM INHIBITORY CONCENTRATION; MISMATCH AMPLIFICATION MUTATION ASSAY; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PARC GENE; POLYMERASE CHAIN REACTION; PROMOTER REGION; QEPA GENE; QNRB GENE; QNRS GENE; SEROPREVALENCE; BASE MISPAIRING; CELL SURVIVAL; DRUG EFFECTS; ENZYMOLOGY; GENETICS; KLEBSIELLA PNEUMONIAE; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEIC ACID AMPLIFICATION; PROCEDURES;

ANTI-BACTERIAL AGENTS; BASE PAIR MISMATCH; BASE SEQUENCE; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CELL SURVIVAL; CIPROFLOXACIN; DNA GYRASE; DNA MUTATIONAL ANALYSIS; DNA TOPOISOMERASE IV; KLEBSIELLA PNEUMONIAE; MALAYSIA; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEIC ACID AMPLIFICATION TECHNIQUES;

EID: 84900005050     PISSN: 23146133     EISSN: 23146141     Source Type: Journal    
DOI: 10.1155/2014/601630     Document Type: Article

References (53)

1. Lautenbach E., Patel J. B., Bilker W. B., Edelstein P. H., Fishman N. O., Extended-spectrum β -lactamase-producing Escherichia coli and Klebsiella pneumoniae: risk factors for infection and impact of resistance on outcomes. Clinical Infectious Diseases 2001 32 8 1162 1171 2-s2.0-0035871033 10.1086/319757
2. Drlica K., Zhao X., Malik M., Salz T., Kerns R., Dougherty T. J., Pucci M. J., Fluoroquinolone resistance: mechanisms, restrictive dosing, and anti-mutant screening strategies for new compounds. Antibiotic Discovery and Development 2012 New York, NY, USA Springer 485 514
3. Crémet L., Caroff N., Dauvergne S., Reynaud A., Lepelletier D., Corvec S., Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. Pathologie Biologie 2011 59 3 151 156 2-s2.0-79958707319 10.1016/j.patbio.2009.04.003
4. Paterson D. L., Mulazimoglu L., Casellas J. M., Ko W.-C., Goossens H., Von Gottberg A., Mohapatra S., Trenholme G. M., Klugman K. P., McCormack J. G., Yu V. L., Epidemiology of ciprofloxacin resistance and its relationship, to extended-spectrum β -lactamase production in Klebsiella pneumoniae isolates causing bacteremia. Clinical Infectious Diseases 2000 30 3 473 478 2-s2.0-0034034589 10.1086/313719
5. Jacoby G. A., Mechanisms of resistance to quinolones. Clinical Infectious Diseases 2005 41 2 S120 S126 2-s2.0-21844477302
6. Deguchi T., Fukuoka A., Yasuda M., Nakano M., Ozeki S., Kanematsu E., Nishino Y., Ishihara S., Ban Y., Kawada Y., Alterations in the gyrA subunit of DNA gyrase and the parC subunit of topoisomerase IV in quinolone-resistant clinical isolates of Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 1997 41 3 699 701 2-s2.0-0031018795
7. Fu Y., Guo L., Xu Y., Zhang W., Gu J., Xu J., Chen X., Zhao Y., Ma J., Liu X., Zhang F., Alteration of GyrA amino acid required for ciprofloxacin resistance in Klebsiella pneumoniae isolates in China. Antimicrobial Agents and Chemotherapy 2008 52 8 2980 2983 2-s2.0-48749111332 10.1128/AAC.00151-08
8. Fu Y., Zhang W., Wang H., Specific patterns of gyrA mutations determine the resistance difference to ciprofloxacin and levofloxacin in Klebsiella pneumoniae and Escherichia coli. BMC Infectious Diseases 2013 13 1, article 8
9. Zirnstein G., Li Y., Swaminathan B., Angulo F., Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. Journal of Clinical Microbiology 1999 37 10 3276 3280 2-s2.0-0032851201
10. Qiang Y. Z., Qin T., Fu W., Cheng W. P., Li Y. S., Yi G., Use of a rapid mismatch PCR method to detect gyrA and parC mutations in ciprofloxacin-resistant clinical isolates of Escherichia coli. Journal of Antimicrobial Chemotherapy 2002 49 3 549 552 2-s2.0-0036125401
11. Sultan Z., Nahar S., Wretlind B., Lindback E., Rahman M., Comparison of mismatch amplification mutation assay with DNA sequencing for characterization of fluoroquinolone resistance in Neisseria gonorrhoeae. Journal of Clinical Microbiology 2004 42 2 591 594 2-s2.0-1242336865 10.1128/JCM.42.2.591-594.2004
12. Birdsell D. N., Pearson T., Price E. P., Hornstra H. M., Nera R. D., Stone N., Gruendike J., Kaufman E. L., Pettus A. H., Hurbon A. N., Buchhagen J. L., Harms N. J., Chanturia G., Gyuranecz M., Wagner D. M., Keim P. S., Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models. PLoS ONE 2012 7 3 2-s2.0-84858397328 10.1371/journal.pone.0032866 e32866
13. Hong B. K., Chi H. P., Chung J. K., Kim E. C., Jacoby G. A., Hooper D. C., Prevalence of plasmid-mediated quinolone resistance determinants over a 9-year period. Antimicrobial Agents and Chemotherapy 2009 53 2 639 645 2-s2.0-59749100468 10.1128/AAC.01051-08
14. Ruiz J., Pons M. J., Gomes C., Transferable mechanisms of quinolone resistance. International Journal of Antimicrobial Agents 2012 40 3 196 203
15. Wang A., Yang Y., Lu Q., Wang Y., Chen Y., Deng L., Ding H., Deng Q., Zhang H., Wang C., Liu L., Xu X., Wang L., Shen X., Presence of qnr gene in Escherichia coli and Klebsiella pneumoniae resistant to ciprofloxacin isolated from pediatric patients in China. BMC Infectious Diseases 2008 8 1, article 68 2-s2.0-44949230200 10.1186/1471-2334-8-68
16. Li B., Yi Y., Wang Q., Analysis of drug resistance determinants in Klebsiella pneumoniae isolates from a tertiary-care hospital in Beijing, China. PLOS ONE 2012 7 7 e42280
17. Shin S. Y., Kwon K. C., Park J. W., Song J. H., Ko Y. H., Sung J. Y., Shin H. W., Koo S. H., Characteristics of aac(6′)-Ib-cr gene in extended-spectrum β -lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from Chungnam area. Korean Journal of Laboratory Medicine 2009 29 6 541 550 2-s2.0-77951463634 10.3343/kjlm.2009.29.6.541
18. Yang H., Chen H., Yang Q., Chen M., Wang H., High prevalence of plasmid-mediated quinolone resistance genes qnr and aac(6′)-Ib-cr in clinical isolates of Enterobacteriaceae from nine teaching hospitals in China. Antimicrobial Agents and Chemotherapy 2008 52 12 4268 4273 2-s2.0-57049169361 10.1128/AAC.00830-08
19. Yamane K., Wachino J. I., Suzuki S., Kimura K., Shibata N., Kato H., Shibayama K., Konda T., Arakawa Y., New plasmid-mediated fluoroquinolone efflux pump, qepA, found in an Escherichia coli clinical isolate. Antimicrobial Agents and Chemotherapy 2007 51 9 3354 3360 2-s2.0-35348894748 10.1128/AAC.00339-07
20. Kim E. S., Jeong J. Y., Choi S. H., Lee S.-O., Kim S.-H., Kim M.-N., Woo J. H., Kim Y. S., Plasmid-mediated fluoroquinolone efflux pump gene, qepA, in Escherichia coli clinical isolates in Korea. Diagnostic Microbiology and Infectious Disease 2009 65 3 335 338 2-s2.0-71749087894 10.1016/j.diagmicrobio. 2009.07.006
21. Liu J. H., Deng Y. T., Zeng Z. L., Gao J.-H., Chen L., Arakawa Y., Chen Z.-L., Coprevalence of plasmid-mediated quinolone resistance determinants qepA, Qnr, and AAC(6′)-Ib-cr among 16S rRNA methylase RmtB-producing Escherichia coli isolates from pigs. Antimicrobial Agents and Chemotherapy 2008 52 8 2992 2993 2-s2.0-48749120335 10.1128/AAC.01686-07
22. Ma J., Zeng Z., Chen Z., Xu X., Wang X., Deng Y., Lü D., Huang L., Zhang Y., Liu J., Wang M., High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6′)-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals. Antimicrobial Agents and Chemotherapy 2009 53 2 519 524 2-s2.0-59649124028 10.1128/AAC.00886-08
23. Luo Y., Yang J., Zhang Y., Ye L., Wang L., Guo L., Prevalence of β -lactamases and 16S rRNA methylase genes amongst clinical Klebsiella pneumoniae isolates carrying plasmid-mediated quinolone resistance determinants. International Journal of Antimicrobial Agents 2011 37 4 352 355 2-s2.0-79952817476 10.1016/j.ijantimicag.2010.12.018
24. Liu Y., Liu C., Zheng W., Zhang X., Yu J., Gao Q., Hou Y., Huang X., PCR detection of Klebsiella pneumoniae in infant formula based on 16S-23S internal transcribed spacer. International Journal of Food Microbiology 2008 125 3 230 235 2-s2.0-48949115263 10.1016/j.ijfoodmicro.2008.03.005
25. Performance Standards for Antimicrobial Susceptibility Testing 2013 Wayne, Pa, USA Clinical and Laboratory Standards Institute document M100-S23
26. Kwok S., Kellogg D. E., McKinney N., Spasic D., Goda L., Levenson C., Sninsky J. J., Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Research 1990 18 4 999 1005 2-s2.0-0025305576
27. Parsons B. L., Heflich R. H., Genotypic selection methods for the direct analysis of point mutations. Mutation Research 1997 387 2 97 121 2-s2.0-0030777020 10.1016/S1383-5742(97)00026-4
28. Huang T. M., Chang Y. F., Chang C. F., Detection of mutations in the gyrA gene and class I integron from quinolone-resistant Salmonella enterica serovar Choleraesuis isolates in Taiwan. Veterinary Microbiology 2004 100 3-4 247 254 2-s2.0-2442459097 10.1016/j.vetmic.2004.03.003
29. Saiful Anuar A. S., Mohd Yusof M. Y., Tay S. T., Prevalence of plasmid-mediated qnr determinants and gyrase alteration in Klebsiella pneumoniae isolated from a university teaching hospital in Malaysia. European Review for Medical and Pharmacological Sciences 2013 17 13 1744 1747
30. Cavaco L. M., Hasman H., Xia S., Aarestrup F. M., qnrD, a novel gene conferring transferable quinolone resistance in Salmonella enterica serovar Kentucky and Bovismorbificans strains of human origin. Antimicrobial Agents and Chemotherapy 2009 53 2 603 608 2-s2.0-59749091684 10.1128/AAC.00997-08
31. Wareham D. W., Umoren I., Khanna P., Gordon N. C., Allele-specific polymerase chain reaction (PCR) for rapid detection of the aac(6′)-Ib-cr quinolone resistance gene. International Journal of Antimicrobial Agents 2010 36 5 476 477 2-s2.0-77957669508 10.1016/j.ijantimicag.2010.07.012
32. Tamang M. D., Seol S. Y., Oh J. Y., Plasmid-mediated quinolone resistance determinants qnrA, qnrB, and qnrS among clinical isolates of Enterobacteriaceae in a Korean hospital. Antimicrobial Agents and Chemotherapy 2008 52 11 4159 4162 2-s2.0-55849133385 10.1128/AAC.01633-07
33. Gautom R. K., Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gram-negative organisms in 1 day. Journal of Clinical Microbiology 1997 35 11 2977 2980 2-s2.0-0030856706
34. Lin C. J., Siu L. K., Ma L., Chang Y. T., Lu P. L., Molecular epidemiology of ciprofloxacin-resistant extended-spectrum β -lactamase-producing Klebsiella pneumoniae in Taiwan. Microbial Drug Resistance 2012 18 1 52 58 2-s2.0-84856739297 10.1089/mdr.2011.0060
35. Dashti A. A., Paton R., Amyes S. G. B., Linkage of ciprofloxacin resistance with a single genotypic cluster of Klebsiella pneumoniae. International Journal of Antimicrobial Agents 2006 27 1 73 76 2-s2.0-29944437224 10.1016/j.ijantimicag.2005.09.007
36. Hsueh P. R., Study for Monitoring Antimicrobial Resistance Trends (SMART) in the Asia-Pacific region, 2002-2010. International Journal of Antimicrobial Agents 2012 40 supplement 1 S1 S3
37. Lim K. T., Yeo C. C., Yasin R. M., Balan G., Thong K. L., Characterization of multidrug-resistant and extended-spectrum β -lactamase-producing Klebsiella pneumoniae strains from Malaysian hospitals. Journal of Medical Microbiology 2009 58 11 1463 1469 2-s2.0-70350519872 10.1099/jmm.0.011114-0
38. Liao C. H., Hsueh P. R., Jacoby G. A., Hooper D. C., Risk factors and clinical characteristics of patients with qnr -positive Klebsiella pneumoniae bacteraemia. Journal of Antimicrobial Chemotherapy 2013 68 12 2907 2914
39. Schneiders T., Amyes S. G. B., Levy S. B., Role of AcrR and RamA in fluoroquinolone resistance in clinical Klebsiella pneumoniae isolates from Singapore. Antimicrobial Agents and Chemotherapy 2003 47 9 2831 2837 2-s2.0-0041422177 10.1128/AAC.47.9.2831-2837.2003
40. Bansal S., Tandon V., Contribution of mutations in DNA gyrase and topoisomerase IV genes to ciprofloxacin resistance in Escherichia coli clinical isolates. International Journal of Antimicrobial Agents 2011 37 3 253 255 2-s2.0-79951956663 10.1016/j.ijantimicag.2010.11.022
41. Drago L., Nicola L., Mattina R., de Vecchi E., In vitro selection of resistance in Escherichia coli and Klebsiella spp. at in vivo fluoroquinolone concentrations. BMC Microbiology 2010 10 1, article 119 2-s2.0-77951083458 10.1186/1471-2180-10-119
42. Mazzariol A., Zuliani J., Cornaglia G., Rossolini G. M., Fontana R., AcrAB efflux system: expression and contribution to fluoroquinolone resistance in Klebsiella spp. Antimicrobial Agents and Chemotherapy 2002 46 12 3984 3986 2-s2.0-0036895630 10.1128/AAC.46.12.3984-3986.2002
43. Ruiz J., Mechanisms of resistance to quinolones: target alterations, decreased accumulation and DNA gyrase protection. Journal of Antimicrobial Chemotherapy 2003 51 5 1109 1117 2-s2.0-0038440791 10.1093/jac/dkg222
44. Pasom W., Chanawong A., Lulitanond A., Plasmid-mediated quinolone resistance genes, aac(6')-Ib-cr, qnrS, qnrB, and qnrA, in urinary isolates of Escherichia coli and Klebsiella pneumoniae at a Teaching Hospital, Thailand. Japanese Journal of Infectious Diseases 2013 66 5 428 432
45. Jiang Y., Zhou Z., Qian Y., Wei Z., Yu Y., Hu S., Li L., Plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in extended-spectrum β -lactamase-producing Escherichia coli and Klebsiella pneumoniae in China. Journal of Antimicrobial Chemotherapy 2008 61 5 1003 1006 2-s2.0-42149117744 10.1093/jac/dkn063
46. Teo J. W. P., Ng K. Y., Lin R. T. P., Detection and genetic characterisation of qnrB in hospital isolates of Klebsiella pneumoniae in Singapore. International Journal of Antimicrobial Agents 2009 33 2 177 180 2-s2.0-58149188041 10.1016/j.ijantimicag.2008.08.019
47. Karah N., Poirel L., Bengtsson S., Sundqvist M., Kahlmeter G., Nordmann P., Sundsfjord A., Samuelsen Ø., Plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in Escherichia coli and Klebsiella spp. from Norway and Sweden. Diagnostic Microbiology and Infectious Disease 2010 66 4 425 431 2-s2.0-77749291953 10.1016/j.diagmicrobio.2009.12.004
48. Pai H., Seo M. R., Choi T. Y., Association of QnrB determinants and production of extended-spectrum β -lactamases or plasmid-mediated AmpC β -lactamases in clinical isolates of Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 2007 51 1 366 368 2-s2.0-33846028571 10.1128/AAC.00841-06
49. Fuursted K., Schumacher H., Significance of low-level resistance to ciprofloxacin in Klebsiella pneumoniae and the effect of increased dosage of ciprofloxacin in vivo using the rat granuloma pouch model. Journal of Antimicrobial Chemotherapy 2002 50 3 421 424 2-s2.0-0036736479
50. Rodríguez-Martínez J. M., Pichardo C., García I., Pachón-Ibañez M. E., Docobo-P.F. P. F., Pascual A., Pachón J., Martínez-Martínez L., Activity of ciprofloxacin and levofloxacin in experimental pneumonia caused by Klebsiella pneumoniae deficient in porins, expressing active efflux and producing QnrA1. Clinical Microbiology and Infection 2008 14 7 691 697 2-s2.0-45249123438 10.1111/j.1469-0691.2008. 02020.x
51. Briales A., Rodríguez-Martínez J. M., Velasco C., Díaz De Alba P., Domínguez-Herrera J., Pachón J., Pascual A., In vitro effect of qnrA1, qnrB1, and qnrS1 genes on fluoroquinolone activity against isogenic Escherichia coli isolates with mutations in gyrA and parC. Antimicrobial Agents and Chemotherapy 2011 55 3 1266 1269 2-s2.0-79952341314 10.1128/AAC.00927-10
52. Rodríguez-Martínez J. M., Velasco C., García I., Cano M. E., Martínez-Martínez L., Pascual A., Mutant prevention concentrations of fluoroquinolones for Enterobacteriaceae expressing the plasmid-carried quinolone resistance determinant qnrA1. Antimicrobial Agents and Chemotherapy 2007 51 6 2236 2239 2-s2.0-34250191805 10.1128/AAC.01444-06
53. van Hees B. C., Tersmette M., Willems R. J. L., de Jong B., Biesma D., van Hannen E. J., Molecular analysis of ciprofloxacin resistance and clonal relatedness of clinical Escherichia coli isolates from haematology patients receiving ciprofloxacin prophylaxis. Journal of Antimicrobial Chemotherapy 2011 66 8 1739 1744 2-s2.0-79960440336 10.1093/jac/dkr216