Nested-quantitative PCR approach with improved sensitivity for the detection of low titer levels of Candidatus Liberibacter asiaticus in the Asian citrus psyllid, Diaphorina citri Kuwayama

Journal of Microbiological Methods

Volumn 102, Issue , 2014, Pages 15-22

  Coy, M R ^a   Hoffmann, M ^a   Kingdom Gibbard, H N ^a   Kuhns, E H ^a   Pelz Stelinski, K S ^a   Stelinski, L L ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

False negatives; Huanglongbing; Low abundance template; Microbial detection methods; Plant pathogen; Template secondary structure

Indexed keywords

DNA 16S; PLASMID DNA; WNT1 PROTEIN; BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

ALPHAPROTEOBACTERIA; ARTICLE; BACTERIUM DETECTION; CANDIDATUS LIBERIBACTER ASIATICUS; CONTROLLED STUDY; DIAPHORINA CITRI; DNA TEMPLATE; FALSE NEGATIVE RESULT; GENE AMPLIFICATION; GENE DOSAGE; GENE TARGETING; NONHUMAN; NUCLEOTIDE BINDING SITE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REACTION OPTIMIZATION; SENSITIVITY ANALYSIS; STRUCTURE ANALYSIS; ANIMAL; CITRUS; GENETICS; HEMIPTERA; HUMAN; INSECT; ISOLATION AND PURIFICATION; MICROBIOLOGY; PLASMID; PROCEDURES; REAL TIME POLYMERASE CHAIN REACTION; RHIZOBIACEAE; SENSITIVITY AND SPECIFICITY;

ANIMALS; CITRUS; DNA, BACTERIAL; DNA, RIBOSOMAL; HEMIPTERA; HUMANS; INSECTS; PLASMIDS; POLYMERASE CHAIN REACTION; REAL-TIME POLYMERASE CHAIN REACTION; RHIZOBIACEAE; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 84899810061     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2014.04.007     Document Type: Article

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