Development of primers and probes for genus and species specific detection of 'Candidatus Liberibacter species' by real-time PCR

Plant Disease

Volumn 97, Issue 9, 2013, Pages 1235-1243

  Ananthakrishnan, G ^a   Choudhary, N ^a   Roy, Avijit ^a   Sengoda, V G ^b   Postnikova, E ^b   Hartung, J S ^b   Stone, A L ^b   Damsteegt, V D ^b   Schneider, W L ^b   Munyaneza, J E ^b   Brlansky, R H ^a  

^a UNIVERSITY OF FLORIDA   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84881496277     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-12-12-1174-RE     Document Type: Article

References (35)

1. Adékambi, T., Drancourt, M., and Raoult, D. 2009. The rpoB gene as a tool for clinical microbiologists. Trends Microbiol. 17:37-45.
2. Borukhov, S., and Nudler, E. 2003. RNA polymerase holoenzyme: Structure, function and biological implications. Curr. Opin. Microbiol. 6:93-100.
3. Case, R. J., Boucher, Y., Dahllöf, I., Holmström, C., Doolittle, W. F., and Kjelleberg, S. 2007. Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Appl. Environ. Microbiol. 73:278-288.
4. Chen, J., Pu, X., Deng, X., Liu, S., Li, H., and Civerolo, E. 2009. A phytoplasma related to 'Candidatus Phytoplasma asteris' detected in citrus showing huanglongbing (yellow shoot disease) symptoms in Guangdong, P. R. China. Phytopathology 99:236-242.
5. Crosslin, J. M., Lin, H., and Munyaneza, J. E. 2011. Detection of 'Candidatus Liberibacter Solanacearum' in the Potato Psyllid, Bactericera cockerelli (Sulc), by Conventional and Real-Time PCR. Southwestern Entomol. 36:125-135.
6. Dahllöf, I., Baillie, H., and Kjelleberg, S. 2000. rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity. Appl. Environ. Microbiol. 66:3376-3380.
7. Damsteegt, V. D., Postnikova, E. N., Stone, A. L., Kuhlmann, M., Wilson, C., Sechler, A. J., Schaad, N. W., Brlansky, R. H., and Schneider, W. L. 2010. Murraya paniculata and related species as potential host and inoculum reservoirs of 'Candidatus Liberibacter asiaticus', causal agent of Huanglongbing. Plant Dis. 94:528-533.
8. Drancourt, M., and Raoult, D. 2002. rpoB gene sequence-based identification of Staphylococcus species. J. Clin. Microbiol. 40:1333-1338.
9. Duan, Y. P., Zhou, L. J., Hall, D., Li, W. B., Doddapaneni, H., Lin, H., Liu, L., Vahling, C. M., Gabriel, D., Williams, K. P., Dickerman, A., Sun, Y., and Gottwald, T. R. 2009. Complete genome sequence of citrus huanglongbing bacterium, 'Candidatus Liberibacter asiaticus' obtained through metagenomics. Mol. Plant-Microbe Interact. 22:1011-1020.
10. Gottwald, T. R., da Graça, J. V., and Bassanezi, R. B. 2007. Citrus huanglongbing: The pathogen and its impact. Plant Health Progress doi:10.1094/PHP-2007-0906-01-RV
11. Halbert, S. E., and Manjunath, K. L. 2004. Asian citrus pysllids (Sternorrhycha: Psyllidae) and greening disease of citrus: A literature review and assessment of risk in Florida. Fla. Entomol. 87:330-353.
12. Hansen, A. K., Trumble, J. T., Stouthamer, R., and Paine, T. D. 2008. A new huanglongbing species, 'Candidatus Liberibacter psyllaurous,' found to infect tomato and potato, is vectored by the psyllid Bactericera cockerelli (Sulc). Appl. Environ. Microbiol. 74:5862-5865.
13. Jagoueix, S., Bové, J. M., and Garnier, M. 1996. PCR detection of the two 'Candidatus' Liberobacter species associated with greening disease of citrus. Mol. Cell. Probes 10:43-50.
14. Jin, D. J., and Gross, C. A. 1989. Three rpoBC mutations that suppress the termination defects of rho mutants also affect the functions of nusA mutants. Mol. Gen. Genet. 216:269-275.
15. Kim, B. J., Lee, S. H., Lyu, M. A., Kim, S. J., Bai, G. H., Chae, G. T., Kim, E. C., Cha, C. Y., and Kook, Y. H. 1999. Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J. Clin. Microbiol. 37:1714-1720.
16. Kim, J. S., and Wang, N. 2009. Characterization of copy numbers of 16S rDNA and 16S rRNA of Candidatus Liberibacter asiaticus and the implication in detection in planta using quantitative PCR. BMC Research Notes 2009, doi:10.1186/1756-0500-2-37
17. Ko, K. S., Lee, H. K., Park, M. Y., Lee, K. H., Yun, Y. J., Woo, S. Y., Miyamoto, H., and Kook, Y. H. 2002. Application of RNA polymerase beta-subunit gene (rpoB) sequences for the molecular differentiation of Legionella species. J. Clin. Microbiol. 40:2653-2658.
18. Lee, S. H., Kim, B. J., Kim, J. H., Park, K. H., Kim, S. J., and Kook, Y. H. 2000. Differentiation of Borrelia burgdorferi sensu lato on the basis of RNA polymerase gene (rpoB) sequences. J. Clin. Microbiol. 38:2557-2562.
19. Li, W., Hartung, J. S., and Levy, L. 2006. Quantitative real-time PCR for detection and identification of 'Candidatus Liberibacter species' associated with citrus huanglongbing. J. Microbiol. Methods 66:104-115.
20. Li, W., Li, D., Tweig, E., Hartung, J. S., and Levy, L. E. 2008. Optimized quantification of unculturable 'Candidatus Liberibacter spp.' in host plants using real-time PCR. Plant Dis. 92:854-861.
21. Liao, X. L., Zhu, S. F., Zhao, W. J., Luo, K., Qi, Y. X., Chen, H. Y., He, K., and Zhu, X. X. 2004. Cloning and sequencing of citrus huanglongbing pathogen 16S rDNA and its detection by real-time fluorescent PCR. J. Agric. Biotechnol. 12:80-85.
22. Liefting, L. W., Weir, B. S., Pennycook, S. R., and Clover, G. R. G. 2009. 'Candidatus Liberibacter solanacearum', associated with plants in the family Solanaceae. Int. J. Syst. Evol. Microbiol. 59:2274-2276.
23. Magomere, T. O., Obukosia, S. D., Mutitu, E., Ngichabe, C., Olubayo, F., and Shibairo, F. 2009. Molecular characterization of 'Candidatus Liberibacter' species/strains causing huanglongbing disease of citrus in Kenya. Electronic J. Biotechnol. Vol. 12, No. 2.
24. Mollet, C., Drancourt, M., and Raoult, D. 1997. rpoB sequence analysis as a novel basis for bacterial identification. Mol. Microbiol. 26:1005-1011.
25. Munyaneza, J. E. 2010. Psyllids as vectors of emerging bacterial diseases of annual crops. Southwestern Entomologist 35(3):471-477.
26. Munyaneza, J. E. 2012. Zebra chip disease of potato: Biology, epidemiology, and management. Am. J. Pot Res. DOI 10.1007/s12230-012-9262-3
27. Munyaneza, J. E., Fisher, T. W., Sengoda, V. G., Garczynski, S. F., Nissinen, A., and Lemmetty, A. 2010. Association of "Candidatus Liberibacter solanacearum" with the psyllid Trioza apicalis (Hemiptera: Triozidae). Eur. J. Econ. Entomol. 103:1060-1070.
28. Munyaneza, J. E., Fisher, T. W., Sengoda, V. G., Garczynski, S. F., Nissinen, A., and Lemmetty, A. 2010. First report of "Candidatus Liberibacter solanacearum" in carrots in Europe. Plant Dis. 94:639.
29. Okuda, M., Matsumoto, M., Tanaka, Y., Subandiyah, S., and Iwanami, T. 2005. Characterization of the tufB-secE-nusG-rplKAJLrpoB gene cluster of the citrus greening organism and detection by loop-mediated isothermal amplification. Plant Dis. 89:705-711.
30. Renesto, P., Gouvernet, J., Drancourt, M., Roux, V., and Raoult, D. 2001. Use of rpoB gene analysis for detection and identification of Bartonella species. J. Clin. Microbiol. 39:430-437.
31. Teixeira, D. C., Saillard, C., Couture, C., Martins, E. C., Wulff, N. A., Jagoueix, S. E., Yamamoto, P. T., Ayres, A. J., and Bové, J. M. 2008. Distribution and quantification of 'Candidatus Liberibacter americanus', agent of huanglongbing disease of citrus in São Paulo State, Brazil, in leaves of an affected sweet orange tree as determined by PCR. Mol. Cell. Probes 22:139-150.
32. Teixeira, D. C., Wulff, N. A., Martins, E. C., Kitajima, E. W., Bassanezi, R., Ayres, A. J., Eveillard, S., Saillard, C., and Bové, J. M. 2008. A phytoplasma closely related to the pigeon pea witches'-broom phytoplasma (16Sr IX) is associated with citrus huanglongbing symptoms in the state of São Paulo, Brazil. Phytopathology 98:977-984.
33. Tian, Y., Ke, S., and Ke, C. 1996. Polymerase chain reaction for detection and quantification of Liberobacter asiaticum, the bacterium associated with huanglongbing (greening) of citrus in China. Pages 252-257 in: Proc. 13th Conf. Int. Organ. Citrus Virol. J. V. da Graça, P. Moreno, and R. K. Yokomi, eds. International Organization of Citrus Virologists, Riverside, CA.
34. Villechanoux, S., Garnier, M., Renaudin, J., and Bové, J. M. 1992. Detection of several strains of the bacterial-like organism of citrus greening disease by DNA probes. Curr. Microbiol. 24:89-95.
35. Wang, Z., Yin, Y., Hu, H., Yuan, Q., Peng, G., and Xia, Y. 2006. Development and application of molecular-based diagnosis for 'Candidatus Liberibacter asiaticus', the causal pathogen of citrus huanglongbing. Plant Pathol. 55:630-638.