Comparison of two methods of extracting bone collagen for stable carbon and nitrogen isotope analysis: Comparing whole bone demineralization with gelatinization and ultrafiltration

Journal of Archaeological Science

Volumn 47, Issue 1, 2014, Pages 64-69

  Sealy, Judith ^a   Johnson, Malia ^a   Richards, Michael ^b,c,d   Nehlich, Olaf ^b,c  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b MAX PLANCK INSTITUTE FOR EVOLUTIONARY ANTHROPOLOGY   (Germany)

^c UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^d DURHAM UNIVERSITY   (United Kingdom)

Author keywords

Carbon; Collagen extraction method; Nitrogen; Palaeodiet; Stable isotope

Indexed keywords

ARCHAEOLOGICAL EVIDENCE; BONE; CARBON ISOTOPE; COLLAGEN; DIET; EXTRACTION METHOD; ISOTOPIC ANALYSIS; MINERALIZATION; NITROGEN ISOTOPE; PROTEIN; STABLE ISOTOPE; ULTRAFILTRATION;

SOUTH AFRICA;

ANIMALIA;

EID: 84899787490     PISSN: 03054403     EISSN: 10959238     Source Type: Journal    
DOI: 10.1016/j.jas.2014.04.011     Document Type: Article

References (31)

1. Ambrose S.H. Preparation and characterization of bone and tooth collagen for isotopic analysis. J.Archaeol. Sci. 1990, 17:431-451.
2. Berger R., Horney A.G., Libby W.F. Radiocarbon dating of bone and shell from their organic components. Science 1964, 144:995-1001.
3. Brock F., Higham T., Ditchfield P., Bronk Ramsey C. Current pretreatment methods for AMS radiocarbon dating at the Oxford Radiocarbon Accelerator Unit (ORAU). Radiocarbon 2010, 52(1):103-212.
4. Brock F., Higham T., Bronk Ramsey C. Comments on the use of Ezee-filters™ and ultrafilters at ORAU. Radiocarbon 2013, 55(1):211-212.
5. Bronk Ramsey C., Higham T., Bowles A., Hedges R. Improvements to the pretreatment of bone at Oxford. Radiocarbon 2004, 46(1):155-163.
6. Brown T.A., Nelson D.E., Vogel J.S., Southon J.R. Improved collagen extraction by modified Longin method. Radiocarbon 1988, 30(2):171-177.
7. Chisholm B.S., Nelson D.E., Hobson K.A., Schwarcz H.P., Knyf M. Carbon isotope measurement techniques for bone collagen: notes for the archaeologist. J.Archaeol. Sci. 1983, 10:355-360.
8. DeNiro M.J. Postmortem preservation and alteration of invivo bone collagen isotope ratios in relation to palaeodietary reconstruction. Nature 1985, 317:806-809.
9. DeNiro M.J., Schoeninger M.J. Stable carbon and nitrogen isotope ratios of bone collagen: variations within individuals, between sexes and within populations raised on monotonous diets. J.Archaeol. Sci. 1983, 10:199-203.
10. DeNiro M.J., Weiner S. Chemical, enzymatic and spectroscopic characterization of "collagen" and other organic fractions from prehistoric bones. Geochim. Cosmochim. Acta 1988, 52:2197-2206.
11. Garvie-Lok S.J., Varney T.L., Katzenberg M.A. Preparation of bone carbonate for stable isotope analysis: the effect of treatment time and acid concentration. J.Archaeol. Sci. 2004, 31:763-776.
12. Haworth R.D. The chemical nature of humic acid. Soil Sci. 1971, 111(1):71-79.
13. Inskeep R.R. Nelson Bay Cave, Cape Province, South Africa: the Holocene Levels 1987, 2 vols. British Archaeol. Reports Intl. Series 357, Oxford.
14. Jørkov M.L.S., Heinemeier J., Lynnerup N. Evaluating bone collagen extraction methods for stable isotope analysis in dietary studies. J.Archaeol. Sci. 2007, 34:1824-1829.
15. Longin R. New method of collagen extraction for radiocarbon dating. Nature 1971, 230:241-242.
16. Nehlich O., Richards M.P. Establishing collagen quality criteria for sulphur isotope analysis of archaeological bone collagen. Archaeol. Anthropol. Sci. 2009, 1:59-75.
17. Pestle W.J. Chemical, elemental, and isotopic effects of acid concentration and treatment duration on ancient bone collagen: an exploratory study. J.Archaeol. Sci. 2010, 37:3124-3128.
18. Richards M.P., Hedges R.E.M. Stable isotope evidence for similarities in the types of marine foods used by Late Mesolithic humans at sites along the Atlantic coast of Europe. J.Archaeol. Sci. 1999, 26:717-722.
19. Richards M.P., Schmitz R. Isotope evidence for the diet of the Neanderthal type specimen. Antiquity 2008, 82:553-559.
20. Richards M.P., Taylor G., Steele T., McPherron S.P., Soressi M., Jaubert J., Orschiedt J., Mallye J.B., Rendu W., Hublin J.J. Isotopic dietary analysis of a Neanderthal and associated fauna from the site of Jonzac (Charente-Maritime), France. J.Hum. Evol. 2008, 55:179-185.
21. Sealy J. Seasonality of rainfall around the Last Glacial Maximum as reconstructed from carbon isotope analyses of animal bones from Nelson Bay Cave. S.Afr. J. Sci. 1996, 92:441-444.
22. Sealy J. Diet, mobility and settlement pattern among Holocene hunter-gatherers in southernmost Africa. Curr. Anthropol. 2006, 47:569-595.
23. Sealy J.C., van der Merwe N.J. Isotope assessment and the seasonal mobility hypothesis in the south-western Cape, South Africa. Curr. Anthropol. 1986, 27:135-150.
24. 13C measurements of Holocene human skeletons from the south-western Cape, South Africa. World Archaeol. 1988, 20:87-102.
25. Sealy J.C., Pfeiffer S. Diet, body size and landscape use among Holocene people in the southern Cape, South Africa. Curr. Anthropol. 2000, 41:642-655.
26. Sealy J., Pfeiffer S., Yates R., Willmore K., Manhire A., Maggs T., Lanham J. Hunter-gatherer child burials from the Pakhuis Mountains, Western Cape: growth, diet and burial practices in the Late Holocene. S.Afr. Archaeol. Bull. 2000, 55:32-43.
27. Sellstedt H., Engstrand L., Gejvall N.-G. New application of radiocarbon dating to collagen residue in bones. Nature 1966, 212:572-574.
28. Steelink C. What is humic acid?. J.Chem. Educ. 1963, 40(7):379-384.
29. Szpak P. Fish bone chemistry and ultrastructure: implications for taphonomy and stable isotope analysis. J.Archaeol. Sci. 2011, 38:3358-3372.
30. Tuross N. Comparative decalcification methods, radiocarbon dates and stable isotopes of the VIRI bones. Radiocarbon 2012, 54(3-4):837-844.
31. Van Klinken G.J. Bone collagen quality indicators for palaeodietary and radiocarbon measurements. J.Archaeol. Sci. 1999, 26:687-695.