Isolation and culture of dental epithelial stem cells from the adult mouse incisor

Journal of Visualized Experiments

Volumn , Issue 87, 2014, Pages

  Chavez, Miquella G ^a   Hu, Jimmy ^a   Seidel, Kerstin ^a   Li, Chunying ^a,b   Jheon, Andrew ^a   Naveau, Adrien ^c,d,e   Horst, Orapin ^a   Klein, Ophir D ^a,f  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b DALIAN UNIVERSITY   (China)

^c UNIVERSITÉ PARIS DESCARTES   (France)

^d CENTRE DE RECHERCHE DES CORDELIERS   (France)

^e INSERM   (France)

^f UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Adult stem cells; Cell culture; Cervical loop; Epithelial stem cells; Incisor; Issue 87; Stem cell biology

Indexed keywords

EID: 84899768132     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/51266     Document Type: Article

References (34)

1. Sharpe, P. T., & Young, C. S. Test-tube teeth. Sci Am. 293, 34-41 (2005).
2. D'Souza, R. N., & Klein, O. D. Unraveling the molecular mechanisms that lead to supernumerary teeth in mice and men: Current concepts and novel approaches. Cells Tissues Organs. 186, 60-69, doi: Doi 10. 1159/000102681 (2007).
3. Jernvall, J., & Thesleff, I. Tooth shape formation and tooth renewal: evolving with the same signals. Development. 139, 3487-3497, doi: Doi 10. 1242/Dev. 085084 (2012).
4. Yen, A. H., & Yelick, P. C. Dental Tissue Regeneration-A Mini-Review. Gerontology. 57, 85-94, doi: Doi 10. 1159/000314530 (2011).
5. Jheon, A. H., Seidel, K., Biehs, B., & Klein, O. D. From molecules to mastication: the development and evolution of tooth development. WIREs Dev Biol. 2, 165-182 (2013).
6. Wang, X. P. et al. An integrated gene regulatory network controls stem cell proliferation in teeth. PLoS Biol. 5, e159 (2007).
7. Harada, H. et al. Localization of putative stem cells in dental epithelium and their association with Notch and FGF signaling. J Cell Biol. 147, 105-120 (1999).
8. Juuri, E. et al. Sox2+ stem cells contribute to all epithelial lineages of the tooth via Sfrp5+ progenitors. Dev Cell. 23, 317-328, doi: 10. 1016/j. devcel. 2012. 05. 012 S1534-5807(12)00239-0 [pii] (2012).
9. Seidel, K. et al. Hedgehog signaling regulates the generation of ameloblast progenitors in the continuously growing mouse incisor. Development. 137, 3753-3761, doi: 137/22/3753 [pii] 10. 1242/dev. 056358 (2010).
10. Smith, C. E., & Warshawsky, H. Cellular renewal in the enamel organ and the odontoblast layer of the rat incisor as followed by radioautography using 3H-thymidine. Anat Rec. 183, 523-561 (1975).
11. Smith, C. E., & Warshawsky, H. Quantitative analysis of cell turnover in the enamel organ of the rat incisor. Evidence for ameloblast death immediately after enamel matrix secretion. Anat Rec. 187, 63-98 (1977).
12. Parsa, S. et al. Signaling by FGFR2b controls the regenerative capacity of adult mouse incisors. Development. 137, 3743-3752, doi: 10. 1242/dev. 051672 137/22/3743 [pii] (2010).
13. Chang, J. Y. et al. Self-renewal and multilineage differentiation of mouse dental epithelial stem cells. Stem Cell Res. 11, 990-1002, doi: S1873-5061(13)00081-0 [pii] 10. 1016/j. scr. 2013. 06. 008 (2013).
14. Biehs, B. et al. Bmi1 represses Ink4a/Arf and Hox genes to regulate stem cells in the rodent incisor. Nat Cell Biol. 15, 846-52 (2013).
15. Hwang, W. S., & Tonna, E. A. Autoradiographic Analysis of Labeling Indices and Migration Rates of Cellular Component of Mouse Incisors Using Tritiated Thymidine (H3tdr). J Dent Res. 44, 42-53 (1965).
16. Li, C. Y. et al. E-cadherin regulates the behavior and fate of epithelial stem cells and their progeny in the mouse incisor. Dev Biol. 366, 357-366, doi: S0012-1606(12)00147-9 [pii] 10. 1016/j. ydbio. 2012. 03. 012 (2012).
17. Smith, C. E. Cellular and chemical events during enamel maturation. Crit Rev Oral Biol Med. 9, 128-161 (1998).
18. Lapthanasupkul, P. et al. Ring1a/b polycomb proteins regulate the mesenchymal stem cell niche in continuously growing incisors. Dev Biol. 367, 140-153, doi: S0012-1606(12)00218-7 [pii] 10. 1016/j. ydbio. 2012. 04. 029 (2012).
19. Chavez, M. G. et al. Characterization of dental epithelial stem cells from the mouse incisor with two-dimensional and three-dimensional platforms. Tissue Eng Part C Methods. 19, 15-24, doi: 10. 1089/ten. TEC. 2012. 0232 (2013).
20. Kawano, S. et al. Establishment of dental epithelial cell line (HAT-7) and the cell differentiation dependent on Notch signaling pathway. Connect Tissue Res. 43, 409-412 (2002).
21. Briggaman, R. A., Abele, D. C., Harris, S. R., & Wheeler, C. E., Jr. Preparation and characterization of a viable suspension of postembryonic human epidermal cells. J Invest Dermatol. 48, 159-168 (1967).
22. Fusenig, N. E. Isolation and cultivation of epidermal cells from embryonic mouse skin. Naturwissenschaften. 58, 421 (1971).
23. Fusenig, N. E., & Worst, P. K. Mouse epidermal cell cultures. I. Isolation and cultivation of epidermal cells from adult mouse skin. J Invest Dermatol. 63, 187-193 (1974).
24. Rheinwald, J. G., & Green, H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 6, 331-343 (1975).
25. Barrandon, Y., & Green, H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci U S A. 84, 2302-2306 (1987).
26. Blanpain, C. et al. Self-renewal, multipotency, and the existence of two cell populations within an epithelial stem cell niche. Cell. 118, 635-648, doi: 10. 1016/j. cell. 2004. 08. 012 S0092867404007895 [pii] (2004).
27. Toma, J. G. et al. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol. 3, 778-784, doi: 10. 1038/ncb0901-778 ncb0901-778 [pii] (2001).
28. Nowak, J. A., & Fuchs, E. Isolation and culture of epithelial stem cells. Methods Mol Biol. 482, 215-232, doi: 10. 1007/978-1-59745-060-7-14 (2009).
29. Rheinwald, J. G., & Green, H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature. 265, 421-424 (1977).
30. Brewer, G. J., Torricelli, J. R., Evege, E. K., & Price, P. J. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination. J Neurosci Res. 35, 567-576, doi: 10. 1002/jnr. 490350513 (1993).
31. Lesuisse, C., & Martin, L. J. Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death. J Neurobiol. 51, 9-23, doi: 10. 1002/neu. 10037 [pii] (2002).
32. Felszeghy, S., Suomalainen, M., & Thesleff, I. Notch signalling is required for the survival of epithelial stem cells in the continuously growing mouse incisor. Differentiation. 80, 241-248, doi: S0301-4681(10)00067-8 [pii] 10. 1016/j. diff. 2010. 06. 004 (2010).
33. Fujimori, S. et al. Wnt/beta-catenin signaling in the dental mesenchyme regulates incisor development by regulating Bmp4. Dev Biol. 348, 97-106, doi: S0012-1606(10)01073-0 [pii] 10. 1016/j. ydbio. 2010. 09. 009 (2010).
34. Klein, O. D. et al. An FGF signaling loop sustains the generation of differentiated progeny from stem cells in mouse incisors. Development. 135, 377-385, doi: dev. 015081 [pii] 10. 1242/dev. 015081 (2008).