A transitional hydrolase to glycosynthase mutant by Glu to Asp substitution at the catalytic nucleophile in a retaining glycosidase

Carbohydrate Research

Volumn 389, Issue 1, 2014, Pages 85-92

  Aragunde, Hugo ^a   Castilla, Estela ^a   Biarnés, Xevi ^a   Faijes, Magda ^a   Planas, Antoni ^a  

^a INSTITUT QUÍMIC DE SARRIÀ   (Spain)

Author keywords

Bacillus licheniformis; Glycosynthase; Hydrolase; 1,3 1,4 Glucans; Glucanase

Indexed keywords

BACTERIOLOGY; CARBOXYLATION; HYDROGEN BONDS; NUCLEOPHILES;

BACILLUS LICHENIFORMIS; CATALYTIC NUCLEOPHILES; GENERAL BASE CATALYSIS; GLUCANASE; GLYCOSYNTHASE; HYDROGEN BONDING INTERACTIONS; HYDROLASE; HYDROLASE ACTIVITIES;

HYDROLASES;

ASPARTIC ACID; CARBOXYLIC ACID; ENDO 1,3(4) BETA GLUCANASE; GLUTAMIC ACID; GLYCOCONJUGATE; GLYCOSIDASE; GLYCOSYNTHASE; HYDROLASE; NUCLEOPHILE; OLIGOSACCHARIDE; POLYSACCHARIDE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS LICHENIFORMIS; CATALYSIS; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; HYDROGEN BOND; HYDROLYSIS; IONIC STRENGTH; MOLECULAR LIBRARY; MOLECULAR WEIGHT; NONHUMAN; PH; PKA; POLYMERIZATION; PRIORITY JOURNAL; STATIC ELECTRICITY; BACILLUS; BIOCATALYSIS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MUTATION; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE;

BACILLUS LICHENIFORMIS;

AMINO ACID SUBSTITUTION; BACILLUS; BIOCATALYSIS; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY;

EID: 84899633626     PISSN: 00086215     EISSN: 1873426X     Source Type: Journal    
DOI: 10.1016/j.carres.2014.02.003     Document Type: Article

References (42)

1. D.L. Zechel, and S.G. Withers Acc. Chem. Res. 33 2000 11 18
2. Q. Wang, and S.G. Withers J. Am. Chem. Soc. 117 1995 10137 10138
3. I. Tews, A.C. Vanscheltinga, A. Perrakis, K.S. Wilson, and B.W. Dijkstra J. Am. Chem. Soc. 119 1997 7954 7959
4. L. Wang, and W. Huang Curr. Opin. Chem. Biol. 13 2009 592 600
5. L.F. Mackenzie, Q. Wang, R.A.J. Warren, and S.G. Withers J. Am. Chem. Soc 120 1998 5583 5584
6. C. Malet, and A. Planas FEBS Lett. 440 1998 208 212
7. P.M. Coutinho, and B. Henrissat H.J. Gilbert, G.J. Davies, B. Henrissat, B. Svensson, Recent Advances in Carbohydrate Bioengineering 1999 The Royal Society of Chemistry Cambridge 3 12
8. G. Perugino, A. Trincone, M. Rossi, and M. Moracci Trends Biotechnol. 22 2004 31 37
9. M. Faijes, and A. Planas Carbohydr. Res. 342 2007 1581 1594
10. B. Cobucci-Ponzano, G. Perugino, A. Strazzulli, M. Rossi, and M. Moracci Methods Enzymol. 510 2012 273 300
11. Z. Armstrong, and S.G. Withers Biopolymers 99 2013 666 674
12. A. Planas Biochim. Biophys. Acta 1543 2000 361 382
13. C. Malet, and A. Planas Biochemistry 36 1997 13838 13848
14. A. Planas, M. Faijes, and M. Abel J. Appl. Glycosci. 50 2003 245 251
15. M. Faijes, J.K. Fairweather, H. Driguez, and A. Planas Chem. Eur. J. 7 2001 4651 4655
16. J.K. Fairweather, M. Faijes, H. Driguez, and A. Planas Chem. Biochem. 3 2002 866 873
17. M. Faijes, X. Pérez, O. Pérez, and A. Planas Biochemistry 42 2003 13304 13318
18. R. Faure, M. Saura-Valls, H. Brumer III, A. Planas, S. Cottaz, and H. Driguez J. Org. Chem. 71 2006 5151 5161
19. X. Perez, M. Faijes, and A. Planas Biomacromolecules 12 2011 494 501
20. H. Aragunde, E. Castilla, M. Faijes, and A. Planas Biochem. J. 458 2014 355 363
21. C.G. Cupples, J.H. Miller, and R.E. Huber J. Biol. Chem. 265 1990 5512 5518
22. S.G. Withers, K. Rupitz, D.E. Trimbur, and R.A.J. Warren Biochemistry 31 1992 9979 9985
23. W.W. Wakarchuk, and R.L. Campbell Protein Sci. 3 1994 467 475
24. T. Addington, B. Calisto, M. Alfonso-Prieto, C. Rovira, I. Fita, and A. Planas Proteins 79 2011 365 375
25. M. Faijes, X. Perez, O. Perez, and A. Planas Biochemistry 42 2003 13304 13318
26. J.L. Viladot, E. de Ramon, O. Durany, and A. Planas Biochemistry 37 1998 11332 11342
27. A. Planas, O. Millet, J. Palasí, C. Pallarés, M. Abel, and J.L. Viladot Carbohydr. Res. 310 1998 53 64
28. C.E. Carraher Seymour/Carraher's Polymer Chemistry 2007 CRC Press
29. A. Sali, and T.L. Blundell J. Mol. Biol. 234 1993 779 815
30. X. Biarnes, J. Nieto, A. Planas, and C. Rovira J. Biol. Chem. 281 2006 1432 1441
31. A. Planas, J.L. Viladot, and M. Faijes H.J. Gilbert, G.J. Davies, B. Henrissat, B. Svensson, Recent Advances in Carbohydrate Bioengineering 1999 The Royal Society of Chemistry Cambridge 292 301
32. J.L. Viladot, F. Canals, X. Batllori, and A. Planas Biochem. J. 355 2001 79 86
33. M. Faijes, T. Imai, V. Bulone, and A. Planas Biochem J. 380 2004 635 641
34. X. Biarnes, A. Ardevol, J. Iglesias-Fernandez, A. Planas, and C. Rovira J. Am. Chem. Soc. 133 2011 20301 20309
35. G.J. Davies, A. Planas, and C. Rovira Acc. Chem. Res. 45 2012 308 316
36. J. Wang, S. Pengthaisong, J.R. Cairns, and Y. Liu Biochim. Biophys. Acta 1834 2013 536 545
37. R. Wilcken, M.O. Zimmermann, A. Lange, A.C. Joerger, and F.M. Boeckler J. Med. Chem. 56 2013 1363 1388
38. L.P. McIntosh, G. Hand, P.E. Johnson, M.D. Joshi, M. Korner, L.A. Plesniak, L. Ziser, W.W. Wakarchuk, and S.G. Withers Biochemistry 35 1996 9958 9966
39. T. Keitel, O. Simon, R. Borriss, and U. Heinemann Proc. Natl. Acad. Sci. U.S.A. 90 1993 5287 5291
40. M. Hahn, J. Pons, A. Planas, E. Querol, and U. Heinemann FEBS Lett. 374 1995 221 224
41. O. Gaiser, K. Piotukh, M.N. Ponnuswamy, A. Planas, R. Borriss, and U. Heinemann J. Mol. Biol. 357 2006 1211 1225
42. U. Heinemann, J. Aÿ, O. Gaiser, J.J. Müller, and M.N. Ponnuswamy Biol. Chem. 377 1996 447 454