3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy

Nature Protocols

Volumn 9, Issue 5, 2014, Pages 1083-1101

  Gao, Liang ^a,b   Shao, Lin ^a   Chen, Bi Chang ^a   Betzig, Eric ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ARTIFACT; BACTERIAL CELL; BESSEL BEAM PLANE ILLUMINATION MICROSCOPY; BESSEL BEAM STRUCTURED PLANE ILLUMINATION; BLEACHING; CELLULAR DYNAMICS; CONVENTIONAL 3D LIVE IMAGING TECHNIQUE; DIFFRACTIVE OPTICAL ELEMENT; DYNAMICS; ELECTRON BEAM; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; ILLUMINATION; IMAGE QUALITY; IMAGING SYSTEM; LIGHT RELATED PHENOMENA; MOLECULAR IMAGING; NONHUMAN; OPTICAL INSTRUMENTATION; OPTICAL SECTIONING; OPTICAL TRANSFER FUNCTION; PHOTON; PHOTOTOXICITY; PRIORITY JOURNAL; SELECTIVE PLANE ILLUMINATION MICROSCOPY; SPATIAL RESOLUTION; STRUCTURED ILLUMINATION MICROSCOPY; THREE DIMENSIONAL IMAGING; THREE DIMENSIONAL LIVE FLUORESCENCE IMAGING; TP BESSEL BEAM PLANE ILLUMINATION; CELLS; DEVICES; METABOLISM; PROCEDURES; SINGLE CELL ANALYSIS; THEORETICAL MODEL;

CELLS; IMAGING, THREE-DIMENSIONAL; LIGHTING; MICROSCOPY, FLUORESCENCE; MODELS, THEORETICAL; PHOTOBLEACHING; SINGLE-CELL ANALYSIS;

EID: 84899538596     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2014.087     Document Type: Article

References (26)

1. Siedentopf, H. & Zsigmondy, R. Über Sichtbarmachung und Groessenbestimmung ultramikroskopischer Teilchen, mit besonderer Anwendung auf Goldrubinglaesern. Ann. d. Physik 10, 1-39 (1903).
2. Huisken, J., Swoger, J., Del Bene, F., Wittbrodt, J. & Stelzer, E.H.K. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305, 1007-1009 (2004).
3. Greger, K., Swoger, J. & Stelzer, E.H.K. Basic building units and properties of a fluorescence single plane illumination microscope. Rev. Sci. Instrum. 78, 023705 (2007).
4. Keller, P.J., Schmidt, A.D., Wittbrodt, J. & Stelzer, E.H.K. Reconstruction of zebrafish early embryonic development by scanned light-sheet microscopy. Science 322, 1065-1069 (2008).
5. Tomer, R., Khairy, K., Amat, F. & Keller, P.J. Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy. Nat. Methods 9, 755-763 (2012).
6. Krzic, U., Gunther, S., Saunders, T.E., Streichan, S.J. & Hufnagel, L. Multiview light-sheet microscope for rapid in toto imaging. Nat. Methods 9, 730-733 (2012).
7. Ahrens, M.B., Orger, M.B., Robson, D.N., Li, J.M. & Keller, P.J. Whole-brain functional imaging at cellular resolution using light-sheet microscopy. Nat. Methods 10, 413-420 (2013).
8. Keller, P.J. Imaging morphogenesis: Technological advances and biological insights. Science 340, 1234168 (2013).
9. Keller, P.J. et al. Fast, high-contrast imaging of animal development with scanned light-sheet-based structured-illumination microscopy. Nat. Methods 7, 637-642 (2010).
10. Planchon, T.A. et al. Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination. Nat. Methods 8, 417-423 (2011).
11. Durnin, J., Miceli, J.J. & Eberly, J.H. Comparison of Bessel and Gaussian beams. Opt. Lett. 13, 79-80 (1988).
12. Truong, T.V., Supatto, W., Koos, D.S., Choi, J.M. & Fraser, S.E. Deep and fast live imaging with two-photon scanned light-sheet microscopy. Nat. Methods 8, 757-760 (2011).
13. Neil, M.A.A., Juškaitis, R. & Wilson, T. Method of obtaining optical sectioning by using structured light in a conventional microscope. Opt. Lett. 22, 1905-1907 (1997).
14. Gustafsson, M.G. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J. Microsc. 198, 82-87 (2000).
15. Gustafsson, M.G.L. et al. Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination. Biophys. J. 94, 4957-4970 (2008).
16. Shao, L., Kner, P., Rego, E.H. & Gustafsson, M.G.L. Super-resolution 3D microscopy of live whole cells using structured illumination. Nat. Methods 8, 1044-1046 (2011).
17. Gao, L. et al. Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens. Cell 151, 1370-1385 (2012).
18. Roh-Johnson, M. et al. Triggering a cell shape change by exploiting preexisting actomyosin contractions. Science 335, 1232-1235 (2012).
19. Habib, S.J. et al. A localized Wnt signal orients asymmetric stem cell division in vitro. Science 339, 1445-1448 (2013).
20. Majoul, I.V. et al. Fast structural responses of gap junction membrane domains to AB5 toxins. Proc. Natl. Acad. Sci. USA 110, E4125-4133 (2013).
21. Schermelleh, L., Heintzmann, R. & Leonhardt, H. A guide to super-resolution fluorescence microscopy. J. Cell Biol. 190, 165-175 (2010).
22. Cella Zanacchi, F. et al. Live-cell 3D super-resolution imaging in thick biological samples. Nat. Methods 8, 1047-1049 (2011).
23. Cella Zanacchi, F., Lavagnino, Z., Faretta, M., Furia, L. & Diaspro, A. Light-sheet confined super-resolution using two-photon photoactivation. PLoS ONE 8, e67667 (2013).
24. Friedrich, M., Gan, Q., Ermolayev, V. & Harms, G.S. STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution. Biophys. J. 100, L43-L45 (2011).
25. Fahrbach, F.O., Simon, P. & Rohrbach, A. Microscopy with self-reconstruction beams. Nat. Photon. 4, 780-785 (2010).
26. Bélanger, P.A. & Rioux, M. Ring pattern of a lens-Axicon doublet illuminated by a Gaussian beam. Appl. Opt. 17, 1080-1088 (1978).