Construction of self-replicating subgenomic dengue virus 4 (DENV4) replicon

Methods in Molecular Biology

Volumn 1138, Issue , 2014, Pages 131-150

  Alcaraz Estrada, Sofia L ^a   Del Angel, Rosa ^a   Padmanabhan, Radhakrishnan ^a  

^a NONE

Author keywords

Antiviral small molecule screening; Bicistronic expression; Cap independent translation; Dengue virus serotype 4; Foot and mouth disease virus 2A; Internal ribosome entry site; Neomycin resistance selectable marker; Overlap extension PCR; Polyprotein precursor; Renilla luciferase reporter replicon

Indexed keywords

CIS ACTING ELEMENT; COMPLEMENTARY DNA; RENILLA LUCIFERIN 2 MONOOXYGENASE; TRANS ACTING FACTOR; VIRUS RNA;

5' UNTRANSLATED REGION; ARTICLE; BACTERIUM COLONY; DENGUE VIRUS 4; DNA REPLICATION; ENCEPHALOMYOCARDITIS VIRUS; GENETIC TRANSFECTION; INTERNAL RIBOSOME ENTRY SITE; LABORATORY TEST; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REPLICON; REPORTER GENE; TRANSIENT TRANSFECTION; VIRUS CAPSID; WEST NILE FLAVIVIRUS; YELLOW FEVER FLAVIVIRUS; ANIMAL; CELL LINE; DENGUE VIRUS; ELECTROPORATION; GENE VECTOR; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; GENETICS; HOMOLOGOUS RECOMBINATION; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; PHYSIOLOGY; PLASMID; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; VIRUS GENOME; VIRUS REPLICATION;

DENGUE VIRUS; ENCEPHALOMYOCARDITIS VIRUS; FLAVIVIRIDAE; FLAVIVIRUS; FOOT-AND-MOUTH DISEASE VIRUS; MIRIDAE; RENILLA LUCIFERASE; WEST NILE VIRUS; YELLOW FEVER VIRUS;

ANIMALS; CELL LINE; CLONING, MOLECULAR; DENGUE VIRUS; ELECTROPORATION; GENES, REPORTER; GENETIC VECTORS; GENOME, VIRAL; HOMOLOGOUS RECOMBINATION; HUMANS; LUCIFERASES, RENILLA; MOLECULAR BIOLOGY; PLASMIDS; POLYMERASE CHAIN REACTION; REPLICON; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION, GENETIC; TRANSFORMATION, GENETIC; VIRUS REPLICATION;

EID: 84899497069     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-0348-1_9     Document Type: Article

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