The C-terminal repeat domains of nsP3 from the old world alphaviruses bind directly to G3BP

Journal of Virology

Volumn 88, Issue 10, 2014, Pages 5888-5893

  Panas, Marc D ^a   Ahola, Tero ^b   McInerney, Gerald M ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; NONSTRUCTURAL PROTEIN 3; PROLINE; RASGAP SH3 DOMAIN BINDING PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ALPHA VIRUS; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHIKUNGUNYA ALPHAVIRUS; COMPLEX FORMATION; CONTROLLED STUDY; EMBRYO; FIBROBLAST; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; SEMLIKI FOREST ALPHAVIRUS;

CHIKUNGUNYA VIRUS; HOST-PATHOGEN INTERACTIONS; SEMLIKI FOREST VIRUS; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84899115565     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.00439-14     Document Type: Article

References (22)

1. Anderson P, Kedersha N. 2009. RNA granules: post-transcriptional and epigenetic modulators of gene expression. Nat. Rev. Mol. Cell Biol. 10: 430-436. http://dx.doi.org/10.1038/nrm2694.
2. Kedersha NL, Gupta M, Li W, Miller I, Anderson P. 1999. RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J. Cell Biol. 147:1431-1442. http://dx.doi.org/10.1083/jcb.147.7.1431.
3. Tourriere H, Chebli K, Zekri L, Courselaud B, Blanchard JM, Bertrand E, Tazi J. 2003. The RasGAP-associated endoribonuclease G3BP assembles stress granules. J. Cell Biol. 160:823-831. http://dx.doi.org/10.1083/jcb.200212128.
4. Beckham CJ, Parker R. 2008. P bodies, stress granules, and viral life cycles. Cell Host Microbe 3:206-212. http://dx.doi.org/10.1016/j.chom.2008.03.004.
5. Reineke LC, Lloyd RE. 2013. Diversion of stress granules and P-bodies during viral infection. Virology 436:255-267. http://dx.doi.org/10.1016/j.virol.2012.11.017.
6. McInerney GM, Kedersha NL, Kaufman RJ, Anderson P, Liljeström P. 2005. Importance of eIF2α phosphorylation and stress granule assembly in alphavirus translation regulation. Mol. Biol. Cell 16:3753-3763. http://dx.doi.org/10.1091/mbc.E05-02-0124.
7. Venticinque L, Meruelo D. 2010. Sindbis viral vector induced apoptosis requires translational inhibition and signaling through Mcl-1 and Bak. Mol. Cancer 9:37. http://dx.doi.org/10.1186/1476-4598-9-37.
8. Panas MD, Varjak M, Lulla A, Eng KE, Merits A, Karlsson Hedestam GB, McInerney GM. 2012. Sequestration of G3BP coupled with efficient translation inhibits stress granules in Semliki Forest virus infection. Mol. Biol. Cell 23:4701-4712. http://dx.doi.org/10.1091/mbc.E12-08-0619.
9. Fros JJ, Domeradzka NE, Baggen J, Geertsema C, Flipse J, Vlak JM, Pijlman GP. 2012. Chikungunya virus nsP3 blocks stress granule assembly by recruitment of G3BP into cytoplasmic foci. J. Virol. 86:10873- 10879. http://dx.doi.org/10.1128/JVI.01506-12.
10. Neuvonen M, Kazlauskas A, Martikainen M, Hinkkanen A, Ahola T, Saksela K. 2011. SH3 domain-mediated recruitment of host cell amphiphysins by alphavirus nsP3 promotes viral RNA replication. PLoS Pathog. 7:e1002383. http://dx.doi.org/10.1371/journal.ppat.1002383.
11. Varjak M, Zusinaite E, Merits A. 2010. Novel functions of the alphavirus nonstructural protein nsP3 C-terminal region. J. Virol. 84:2352-2364. http://dx.doi.org/10.1128/JVI.01540-09.
12. Galbraith SE, Sheahan BJ, Atkins GJ. 2006. Deletions in the hypervariable domain of the nsP3 gene attenuate Semliki Forest virus virulence. J. Gen. Virol. 87:937-947. http://dx.doi.org/10.1099/vir.0.81406-0.
13. Oberste MS, Parker MD, Smith JF. 1996. Complete sequence of Venezuelan equine encephalitis virus subtype IE reveals conserved and hypervariable domains within the C terminus of nsP3. Virology 219:314-320. http://dx.doi.org/10.1006/viro.1996.0254.
14. Strauss EG, Levinson R, Rice CM, Dalrymple J, Strauss JH. 1988. Nonstructural proteins nsP3 and nsP4 of Ross River and O'Nyong-nyong viruses: sequence and comparison with those of other alphaviruses. Virology 164:265-274. http://dx.doi.org/10.1016/0042-6822(88)90644-7.
15. Vihinen H, Ahola T, Tuittila M, Merits A, Kaariainen L. 2001. Elimination of phosphorylation sites of Semliki Forest virus replicase protein nsP3. J. Biol. Chem. 276:5745-5752. http://dx.doi.org/10.1074/jbc.M006077200.
16. Breakwell L, Dosenovic P, Karlsson Hedestam GB, D'Amato M, Liljeström P, Fazakerley J, McInerney GM. 2007. Semliki Forest virus nonstructural protein 2 is involved in suppression of the type I interferon response. J. Virol. 81:8677-8684. http://dx.doi.org/10.1128/JVI.02411-06.
17. Foy NJ, Akhrymuk M, Akhrymuk I, Atasheva S, Bopda-Waffo A, Frolov I, Frolova EI. 2013. Hypervariable domains of nsP3 proteins of New World and Old World alphaviruses mediate formation of distinct, virusspecific protein complexes. J. Virol. 87:1997-2010. http://dx.doi.org/10.1128/JVI.02853-12.
18. Foy NJ, Akhrymuk M, Shustov AV, Frolova EI, Frolov I. 2013. Hypervariable domain of nonstructural protein nsP3 of Venezuelan equine encephalitis virus determines cell-specific mode of virus replication. J. Virol. 87:7569-7584. http://dx.doi.org/10.1128/JVI.00720-13.
19. Cristea IM, Carroll JW, Rout MP, Rice CM, Chait BT, MacDonald MR. 2006. Tracking and elucidating alphavirus-host protein interactions. J. Biol. Chem. 281:30269-30278. http://dx.doi.org/10.1074/jbc.M603980200.
20. Frolova E, Gorchakov R, Garmashova N, Atasheva S, Vergara LA, Frolov I. 2006. Formation of nsP3-specific protein complexes during Sindbis virus replication. J. Virol. 80:4122-4134. http://dx.doi.org/10.1128/JVI.80.8.4122-4134.2006.
21. Gorchakov R, Garmashova N, Frolova E, Frolov I. 2008. Different types of nsP3-containing protein complexes in Sindbis virus-infected cells. J. Virol. 82:10088-10101. http://dx.doi.org/10.1128/JVI.01011-08.
22. Garmashova N, Gorchakov R, Volkova E, Paessler S, Frolova E, Frolov I. 2007. The Old World and New World alphaviruses use different virusspecific proteins for induction of transcriptional shutoff. J. Virol. 81: 2472-2484. http://dx.doi.org/10.1128/JVI.02073-06.