Development and characterization of novel agar and gelatin injectable hydrogel as filler for peripheral nerve guidance channels

Journal of Tissue Engineering and Regenerative Medicine

Volumn 11, Issue 1, 2017, Pages 197-208

  Tonda Turo, C ^a   Gnavi, S ^b   Ruini, F ^a   Gambarotta, G ^b   Gioffredi, E ^a   Chiono, V ^a   Perroteau, I ^b   Ciardelli, G ^a,c  

^a POLITECNICO DI TORINO   (Italy)

^b UNIVERSITY OF TURIN   (Italy)

^c CNR   (Italy)

Author keywords

agar; gelatin; glial like cells; hydrogel; injectable; peripheral nerve regeneration

Indexed keywords

ALGAE; BIOMECHANICS; CELL ADHESION; COPYRIGHTS; HYDROGELS; NEURONS; POLYSACCHARIDES; SCAFFOLDS (BIOLOGY); SHEAR FLOW; SHEAR THINNING; SURGERY; TISSUE; TISSUE REGENERATION;

GELATIN; GLIAL-LIKE CELL; IN-VITRO; INJECTABLE HYDROGELS; INJECTABLES; NERVE GUIDANCE CHANNEL; PERIPHERAL NERVE REGENERATION; PERIPHERAL NERVES; PROPERTY; TISSUES ENGINEERINGS;

CELLS;

AGAR; FILLER; GELATIN; GENIPIN; MOLECULAR SCAFFOLD; ALGINIC ACID; IRIDOID;

3T3 CELL LINE; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL MIGRATION; CELL PROLIFERATION; COMPRESSION; CONTROLLED STUDY; DEGRADATION KINETICS; DISSOLUTION; FLOW KINETICS; HYDROGEL; IN VITRO STUDY; INFRARED SPECTROSCOPY; MOUSE; NERVE GUIDANCE CHANNEL; NEUROLOGICAL EQUIPMENT; NONHUMAN; OLFACTORY ENSHEATHING CELL; PRIORITY JOURNAL; RAT; SCHWANN CELL; ANIMAL; APOPTOSIS; CELL MOTION; CELL SURVIVAL; CHEMISTRY; COMPRESSIVE STRENGTH; CYTOLOGY; MATERIALS TESTING; MECHANICAL STRESS; NERVE CELL; NERVE REGENERATION; NIH 3T3 CELL LINE; PH; PROCEDURES; REGENERATION; TISSUE ENGINEERING; TISSUE SCAFFOLD;

AGAR; ALGINATES; ANIMALS; APOPTOSIS; CELL ADHESION; CELL MOVEMENT; CELL PROLIFERATION; CELL SURVIVAL; COMPRESSIVE STRENGTH; GELATIN; HYDROGELS; HYDROGEN-ION CONCENTRATION; IRIDOIDS; MATERIALS TESTING; MICE; NERVE REGENERATION; NEURONS; NIH 3T3 CELLS; RATS; REGENERATION; RHEOLOGY; SCHWANN CELLS; STRESS, MECHANICAL; TISSUE ENGINEERING; TISSUE SCAFFOLDS;

EID: 84899043044     PISSN: 19326254     EISSN: 19327005     Source Type: Journal    
DOI: 10.1002/term.1902     Document Type: Article

References (33)

1. Alzari V, Nuvoli D, Scognamillo S et al. 2011; Graphene-containing nanocomposite hydrogels of poly(Nisopropylacrylamide) prepared by frontal polymerization. J Mater Chem 21: 8727–8733.
2. Balgude AP, Yu X, Szymanski A et al. 2001; Agar gel stiffness determines rate of DRG neurite extension in 3D cultures. Biomaterials 2: 1077–1084.
3. Chiono V, Pulieri E, Vozzi G et al. 2008; Genipin-crosslinked chitosan/gelatin blends for biomedical applications. J Mater Sci Mater Med 19: 889–898.
4. Chiono V, Tonda-Turo C, Ciardelli G. 2009; Artificial scaffolds for peripheral nerve reconstruction. Int Rev Neurobiol 87: 173–199.
5. Cordeiro PG, Seckel BR, Lipton SA et al. 1989; Acidic fibroblast growth factor enhances peripheral nerve regeneration in vivo. Plast Reconstr Surg 83: 1013–1019.
6. Crompton KE, Goud JD, Bellamkonda RV et al. 2007; Polylysine-functionalised thermoresponsive chitosan hydrogel for neural tissue engineering. Biomaterials 28: 441–449.
7. Deligkaris K, Tadele ST, Olthuis W et al. 2010; Hydrogel-based devices for biomedical applications. Sensors Actuat B 147: 765–774.
8. Freile­Pelegrın Y, Murano E. 2005; Agars from three species of Gracilaria (Rhodophyta) from Yucatan Peninsula. Bioresour Technol 96: 295–302.
9. Goodman MN, Silver J, Jacobberger JW. 1993; Establishment and neurite outgrowth properties of neonatal and adult rat olfactory bulb glial cell lines. Brain Res 619: 199–213.
10. Guérout N, Paviot A, Bon-Mardion N et al. 2011; Co-transplantation of olfactory ensheathing cells from mucosa and bulb origin enhances functional recovery after peripheral nerve lesion. PLoS ONE 6: e22816.
11. Guvendiren M, Lu HD, Burdick JA. 2012; Shear-thinning hydrogels for biomedical applications. Soft Matter 8: 260–272.
12. Lyons JG, Geever LM, Nugent MJD et al. 2009; Development and characterisation of an agar–polyvinyl alcohol blend hydrogel. J Mech Behav Biomed 2: 485–493.
13. Macosko C. 1994; Rheology: Principles, Measurements and Application. Wiley/VCH: Poughkeepsie, NY.
14. Madison R, Da Silva CF, Dikkes P et al. 1985; Increased rate of peripheral nerve regeneration using bioresorbable nerve guides and a laminin-containing gel. Exp Neurol 88: 767–772.
15. Mahoney MJ, Anseth KS. 2006; Three-dimensional growth and function of neural tissue in degradable polyethylene glycol hydrogels. Biomaterials 27: 2265–2274.
16. Marten PJ, Bryant SJ, Anseth KS. 2003; Tailoring the degradation of hydrogels formed from multivinyl poly(ethylene glycol) and poly(vinyl alcohol) macromers for cartilage tissue engineering. Biomacromolecules 4: 283–292.
17. Meena R, Prasad K, Siddhanta AK. 2007; Preparation of genipin-fixed agar hydrogel. J Appl Pol Sci 104: 290–296.
18. Muyonga JH, Cole CGB, Duodu KG. 2004; Fourier transform infrared (FTIR) spectroscopic study of acid soluble collagen and gelatin from skins and bones of young and adult Nile perch (Lates niloticus). Food Chem 86: 325–332.
19. Parrinello S, Napoli I, Ribeiro S et al. 2010; EphB signaling directs peripheral nerve regeneration through Sox2-dependent Schwann cell sorting. Cell 143: 145–155.
20. Praiboon J, Chirapart A, Akakabe Y et al. 2006; Physical and chemical characterization of agar polysaccharides extracted from the Thai and Japanese species of Gracilaria. Sci Asia 32: 11–17.
21. Quaglia F. 2008; Bioinspired tissue engineering: the great promise of protein delivery technologies. Int J Pharm 364: 281–297.
22. Radtke C, Kocsis JD, Vogt PM. 2009; Transplantation of olfactory ensheathing cells for peripheral nerve regeneration. Int Rev Neurobiol 87: 405–415.
23. Sakiyama SE, Schense JC, Hubbell JA. 1999; Incorporation of heparin-binding peptides into fibrin gels enhances neurite extension: an example of designer matrices in tissue engineering. FASEB J 13: 2214–2224.
24. Seckel BR, Jones D, Hekimian KJ et al. 1995; Hyaluronic acid through a new injectable nerve guide delivery system enhances peripheral nerve regeneration in the rat. J Neurosci Res 40: 318–324.
25. Slaughter BV, Khurshid SS, Fisher OZ et al. 2009; Hydrogels in regenerative medicine. Adv Mater 21: 3307–3329.
26. Sorrell JM, Caplan AI. 2009, Fibroblasts – a diverse population at the center of it all. Int Rev Cell Mol Biol 276: 161–214.
27. Steinert AF, Weber M, Kunz M et al. 2008; In situ IGF-1 gene delivery to cells emerging from the injured anterior cruciate ligament. Biomaterials 29: 904–916.
28. Tonda-Turo C, Audisio C, Gnavi S et al. 2011a; Porous poly(ε-caprolactone) nerve guide filled with porous gelatin matrix for nerve tissue engineering. Adv Eng Mater 13: B151–B164.
29. Tonda-Turo C, Gentile P, Saracino S et al. 2011b; Comparative analysis of gelatin scaffolds crosslinked by genipin and silane coupling agent. Int J Biol Macromol 49: 700–706.
30. Tonda-Turo C, Cipriani E, Gnavi S et al. 2013; Crosslinked gelatin nanofibres: preparation, characterization and in vitro studies using glial-like cells. Mater Sci Eng C 33: 2723–2735.
31. Wells MR, Kraus K, Batter DK et al. 1997; Gel matrix vehicles for growth factor application in nerve gap injuries repaired with tubes: a comparison of biomatrix, collagen, and methylcellulose. Exp Neurol 146: 395–402.
32. Willert SM, Sakiyama-Elbert SE. 2007; Approaches to neural tissue engineering using scaffolds for drug delivery. Adv Drug Deliv Rev 59: 325–338.
33. Williams LR, Danielsen N, Muller H et al. 1987; Exogenous matrix precursors promote functional nerve regeneration across a 15 mm gap within a silicone chamber in the rat. J Comp Neurol 264: 284–290.