Leaf tissue sampling and DNA extraction protocols

Methods in Molecular Biology

Volumn 1115, Issue , 2014, Pages 53-67

  Semagn, Kassa ^a  

^a INTERNATIONAL MAIZE AND WHEAT IMPROVEMENT CENTER CIMMYT   (Kenya)

Author keywords

Blotter paper; CTAB; Desiccant; DNA extraction; Dry ice; Ethanol; Leaf sampling; Silica gel

Indexed keywords

2 PROPANOL; ALCOHOL; CARBON DIOXIDE; DESICCANT; DNA; LIQUID NITROGEN; RESTRICTION ENDONUCLEASE; SILICA GEL;

ARTICLE; CLINICAL PROTOCOL; DNA EXTRACTION; FREEZING; GENOTYPE; LEAF TISSUE; NONHUMAN; PRIORITY JOURNAL; SPECIES;

CHEMICAL FRACTIONATION; DNA, PLANT; PLANT LEAVES; QUALITY CONTROL;

EID: 84898825582     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-767-9_3     Document Type: Article

References (32)

1. Klimyuk VI, Carroll BJ, Thomas CM, Jones JDG (1993) Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J 3:493-494
2. Clancy JA, Jitkov VA, Han F, Ullrich SE (1996) Barley tissue as direct template for PCR: A practical breeding tool. Mol Breed 2: 181-183
3. Langridge U, Schwall M, Langridge P (1991) Squashes of plant tissue as substrate for PCR. Nucleic Acids Res 19:6954
4. Ikeda N, Bautista NS, Yamada T, Kamijima O, Ishii T (2001) Ultra-simple DNA extraction method for marker-assisted selection using microsatellite markers in rice. Plant Mol Biol Report 19:27-32
5. Lin JJ, Fleming R, Kuo JMBF, Saunders JA (2000) Detection of plant genes using a rapid, nonorganic DNA purifi cation method. Biotechniques 28:346-350
6. Gao S, Martinez C, Skinner DJ, Krivanek AF, Crouch JH, Xu Y (2008) Development of a seed DNA-based genotyping system for marker-assisted selection in maize. Mol Breed 22:477-494
7. Marsal G, Baiges I, Canals JM, Zamora F, Fort F (2011) A fast, effi cient method for extracting DNA from leaves, stems, and seeds of Vitis vinifera L. Am J Enol Vitic 62:376-381
8. Praveen M, Nanna RS (2009) A simple and rapid method for DNA extraction from leaves of tomato, tobacco and rape seed. J Phytol 1:388-390
9. Salim K, Qureshi MI, Kamaluddin, Tanweer A, Abdin MZ (2007) Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion. Afr J Biotechnol 6:175-178
10. Lin J, Ritland K (1995) Flower petals allow simpler and better isolation of DNA for plant RAPD analyses. Plant Mol Biol Report 13: 210-213
11. Reddy GM, Coe E (1990) Isolation of nucleic acids from pollen grains. Maize Genet Coop News Lett 1990, 43
12. Chase MW, Hills HH (1991) Silica gel: An ideal material for fi eld preservation of leaf samples for DNA studies. Taxon 40:215-220
13. Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314-331
14. Elshire RJ, Glaubitz JC, Sun Q, Poland JA, Kawamoto K, Buckler ES et al (2011) A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species. PLoS ONE 6:e19379
15. Rogstad SH (1992) Saturated NaCl-CTAB solution as a means of fi eld preservation of leaves for DNA analyses. Taxon 41:701-708
16. Storchova H, Hrdlickova R, Chrtek J Jr, Tetera M, Fitze D, Fehrer J (2000) An improved method of DNA isolation from plants collected in the fi eld and conserved in saturated NaCl/CTAB solution. Taxon 49:79-84
17. Fukatsu T (1999) Acetone preservation: A practical technique for molecular analysis. Mol Ecol 8:1935-1945
18. Flournoy LE, Adams RP, Pandy RN (1996) Interim and archival preservation of plant specimens in alcohols for DNA studies. Biotechniques 20:657-660
19. Dubreuil P, Warburton M, Chastanet M, Hoisington D, Charcosset A (2006) More on the introduction of temperate maize into Europe: Large-scale bulk SSR genotyping and new historical elements. Maydica 51:281-291
20. Warburton ML, Setimela P, Franco J, Cordova H, Pixley K, Banziger M et al (2010) Toward a cost-effective fi ngerprinting methodology to distinguish maize open-pollinated varieties. Crop Sci 50:467-477
21. Etten JV, Lopez MRF, Monterroso LGM, Samayoa KMP (2008) Genetic diversity of maize (Zea mays L. Ssp. Mays) in communities of the western highlands of Guatemala: Geographical patterns and processes. Genet Resour Crop Evol 55:303-317
22. Eschholz TW, Peter R, Stamp P, Hund A (2008) Genetic diversity of Swiss maize (Zea mays L. Ssp. Mays) assessed with individuals and bulks on agarose gels. Genet Resour Crop Evol 55:971-983
23. Reif JC, Hamrit S, Heckenberger M, Schipprack W, Peter MH, Bohn M et al (2005) Genetic structure and diversity of European fl int maize populations determined with SSR analyses of individuals and bulks. Theor Appl Genet 111:906-913
24. Michelmore RW, Paran I, Kesseli RV (1991) Identifi cation of markers linked to diseaseresistance genes by bulked segregant analysis: A rapid method to detect markers in specifi c genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828-9832
25. Pacek P, Sajantila A, Syvanen AC (1993) Determination of allele frequencies at loci with length polymorphism by quantitative analysis of DNA amplifi ed from pooled samples. PCR Methods Appl 2:313-317
26. Fu YB (2003) Applications of bulking in molecular characterization of plant germplasm: A critical review. Plant Gen Res 1:161-167
27. Wang H, Qi MQ, Cutler AJ (1993) A simple method of preparing plant samples for PCR. Nucleic Acids Res 21:4153-4154
28. Williams CE, Ronald PC (1994) PCR template-DNA isolated quickly from monocot and dicot leaves without tissue homogenization. Nucleic Acids Res 22:1917-1918
29. Post RV, Post LV, Dayteg C, Nilsson M, Forster BP, Tuvesson S (2003) A highthroughput DNA extraction method for barley seed. Euphytica 130:255-260
30. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81:8014-8018
31. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321-4325
32. Paterson AH, Brubaker CL, Wendel JF (1993) A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol Biol Report 11:122-127