New safety and rapid method for extraction of genomic DNA from bacteria and yeast strains suitable for PCR amplifications

Journal of Pure and Applied Microbiology

Volumn 8, Issue 1, 2014, Pages 383-388

  Hesham, Abd El Latif ^a,b  

^a Assiut University   (Egypt)

^b KING KHALID UNIVERSITY   (Saudi Arabia)

Author keywords

Bacteria; Freeze sudden thawing; PCR applications; Safety and rapid DNA extraction; Yeasts

Indexed keywords

BACTERIAL DNA; DNA 16S; DNA 26S; FUNGAL DNA; GENOMIC DNA; TOXIC SUBSTANCE;

ARTICLE; DNA EXTRACTION; DNA FINGERPRINTING; DNA SEQUENCE; FREEZE THAWING; GENE AMPLIFICATION; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; PROKARYOTE; RANDOM AMPLIFIED POLYMORPHIC DNA; RNA SEQUENCE; SACCHAROMYCES; SAFETY; YEAST CELL;

EID: 84898765834     PISSN: 09737510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Ausbel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K. Current Protocols in Molecular Biology. John Wiley and Sons, 1995; 2: 4.1.
2. Paterson, A.H., Brubaker, C.L., Wendel, J.F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis. Plant Mol. Biol. Rep., 1993; 11(2): 122-127.
3. Cheng, H.R., Jiang, N. Extremely rapid extraction of DNA from bacteria and yeasts. Biotechnol. Lett., 2006; 28(1): 55-59. (Pubitemid 41821493)
4. Melo, S.C.O., Pungartnik, C., Cascardo, J.C.M., Brendel, M. Rapid and efficient protocol for DNA extraction and molecular identification of the basidiomycete Crinipellis perniciosa. Genet. Mol. Res., 2006; 5(4): 851-855.
5. Borman, A.M., Linton, C.J., Miles, S.J., Campbell, C.K., Johnson, E.M. Ultrarapid preparation of total genomic DNA from isolates of yeast and mould using Whatman FTA filter paper technology - a reusable DNA archiving system. Med. Mycol., 2006; 44(5): 389-398.
6. Liguori, G., Lucariello, A., Colella, G., Luca, A.D., Marinelli, P. Rapid identification of Candida species in oral rinse solutions by PCR. J. Clin. Pathol., 2007; 60(9): 1035-1039. (Pubitemid 47441938)
7. Martorell, M., Fernandez-Espinar, M.T., Querol, A. Molecular monitoring of spoilage yeasts during the production of candied fruit nougats to determine food contamination sources. Int. J. food Microbiol., 2005; 101: 293-302. (Pubitemid 40733499)
8. Lane, D.J. 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. Wiley, New York, 1991; 115-175.
9. Hesham, A., Mohammed, N.H., Ismail, M.A., Shoreit, A. A. 16S rRNA gene sequences analysis of Ficus elastica rubber latex degrading thermophilic Bacillus strain ASU7 isolated from Egypt. Biodegradation, 2012; 23: 717-724.
10. Kurtzman, C.P., Robnett, C.J. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek, 1998; 73: 331-371. (Pubitemid 28528723)
11. Hesham, A., Khan, S., Liu, X., Zhang, Y., Wang, Z., Yang, M. Application of PCR-DGGE to analyse the yeast population dynamics in slurry reactors during degradation of polycyclic aromatic hydrocarbons in weathered oil. Yeast, 2006; 23: 879-887. (Pubitemid 44649531)
12. Hesham, A., Mohamed, H. Molecular genetic identification of yeast strains isolated from Egyptian soils for solubilization of inorganic phosphates and growth promotion of corn plants. J. Microbiol. Biotechnol., 2011; 21: 55-61.
13. Galán, A., Veses, V., Murgui, A., Casanova, M., Martínez, J.P. Rapid PCR-based test for identifying Candida albicans by using primers derived from the pH regulated KER1 gene. FEMS Yeast Res., 2006; 6(7): 1094-1100. (Pubitemid 44568866)