Puerarin protects endothelial cells from oxidized low density lipoprotein induced injuries via the suppression of LOX-1 and induction of eNOS

Canadian Journal of Physiology and Pharmacology

Volumn 92, Issue 4, 2014, Pages 299-306

  Bao, Mei hua ^a,b   Zhang, Yi wen ^a   Lou, Xiao ya ^a   Xiao, Yan ^c   Cheng, Yu ^a   Zhou, Hong hao ^a  

^a The First People s Hospital of Changde City)   (China)

^b CHANGSHA MEDICAL UNIVERSITY   (China)

^c Qingdao Science and Standard Chemicals Analysing and Testing Co Ltd   (China)

Author keywords

Atherosclerosis; Endothelial cells; eNOS; Lectin like low density lipoprotein receptor 1; Puerarin

Indexed keywords

2',7' DICHLOROFLUORESCEIN DIACETATE; CYCLOOXYGENASE 2; ENDOTHELIAL NITRIC OXIDE SYNTHASE; I KAPPA B; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 8; LACTATE DEHYDROGENASE; MITOGEN ACTIVATED PROTEIN KINASE P38; NITRIC OXIDE; OXIDIZED LOW DENSITY LIPOPROTEIN; OXIDIZED LOW DENSITY LIPOPROTEIN RECEPTOR 1; PROTEIN KINASE B; PUERARIN; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ARTICLE; CELL DAMAGE; CELL PROTECTION; CELL VIABILITY; CYTOKINE RELEASE; DEPHOSPHORYLATION; DRUG MECHANISM; ENDOTHELIUM CELL; ENZYME PHOSPHORYLATION; ENZYME RELEASE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

CELL SURVIVAL; DOWN-REGULATION; ENZYME INDUCTION; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; ISOFLAVONES; LIPOPROTEINS, LDL; NF-KAPPA B; NITRIC OXIDE SYNTHASE TYPE III; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; PROTO-ONCOGENE PROTEINS C-AKT; REACTIVE OXYGEN SPECIES; SCAVENGER RECEPTORS, CLASS E; SIGNAL TRANSDUCTION; UP-REGULATION;

EID: 84898736941     PISSN: 00084212     EISSN: 12057541     Source Type: Journal    
DOI: 10.1139/cjpp-2013-0322     Document Type: Article

References (43)

1. Bao, M., and Lou, Y. 2006. Isorhamnetin inhibits endothelial cell injuries induced by oxidized LDL thro gh activation of p38MAPK. Eur. J. Pharmacol. 547: 22-30. doi:10.1016/j.ejphar.2006.07.021. PMID:16963021.
2. Bao, M.H., Zhang, Y.W., and Zhou, H.H. 2013. Paeonol suppresses oxidized low- density lipoprotein induced endothelial cell apoptosis via activation of LOX- 1/p38MAPK/NF-kB pathway. J. Ethnopharmacol. 146(2): 543-551. doi:10.1016/ j.jep.2013.01.019. PMID:23357312.
3. Celesrmajer, D.S. 1997. Endothelial dysfunction: does it matter? Is it reversible? J. Am. Coll. Cardiol. 30: 325-333. doi:10.1016/S0735-1097(97)00189-7. PMID: 9247501.
4. Chen, Y.Y., Chen, J., Zhou, X.M., Meng, X.H., Jiang, J.P., and Shen, Y.L. 2012. Puerarin protects human umbilical vein endothelial cells against high glucose- induced apoptosis by upregulating heme oxygenase-1 and inhibiting calpain activation. Fundam. Clin. Pharmacol. 26(3): 322-331. doi:10.1111/j.1472-8206. 2011.00926.x. PMID:21352351.
5. Cominacini, L., Pasini, A.F., Garbin, U., Davoli, A., Tosetti, M.L., Campagnola, M., et al. 2000. Oxidized low density lipoprotein (oxLDL) binding to oxLDL receptor-1 in endothelial cells induces the activation of NF-kappaB through an increased production of intracellular reactive oxygen species. J. Biol. Chem. 275(17): 12633-12638. doi:10.1074/jbc.275.17.12633. PMID:10777555.
6. Cominacini, L., Rigoni, A., Pasini, A.F., Garbin, U., Davoli, A., Campagnola, M., et al. 2001. The binding of oxidized low-density lipoprotein (oxLDL) to oxLDL receptor-1 reduces the intracellular concentration of nitric oxide in endothelial cells through an increase production of superoxide. J. Biol. Chem. 276: 13750-13755. PMID:11278710.
7. De Castellarnau, C., Sánchez-Quesada, J.L., Benítez, S., Rosa, R., Caveda, L., Vila, L., and Ordóñez-Llanos, J. 2000. Electronegative LDL from normolipemic subjects induces IL-8 and monocyte chemotactic protein secretion by human endothelial cells. Arterioscler. Thromb. Vasc. Biol. 20: 2281-2287. doi:10.1161/01.ATV.20.10.2281. PMID:11031216.
8. Hirase, T., and Node, K. 2012. Endothelial dysfunction as a cellular mechanism for vascular failure. Am. J. Physiol. Heart Circ. Physiol. 302: H499-H505. doi:10.1152/ajpheart.00325.2011. PMID:22081698.
9. Huang, B., Yang, X.D., Lamb, A., and Chen, L.F. 2010. Posttranslational modifications of NF-kappaB: another layer of regulation for NF-kappaB signaling pathway. Cell. Signal. 22: 1282-1290. doi:10.1016/j.cellsig.2010.03.017. PMID:20363318.
10. Hwang, Y.P., Kim, H.G., Hien, T.T., Jeong, M.H., Jeong, T.C., and Jeong, H.G. 2011. Puerarin activates endothelial nitric oxide synthase through estrogen receptor-dependent PI3-kinase and calcium-dependent AMP-activated protein kinase. Toxicol. Appl. Pharmacol. 257: 48-58. doi:10.1016/j.taap.2011.08. 017. PMID:21884717.
11. Jin, S.E., Son, Y.K., Min, B.S., Jung, H.A., and Choi, J.S. 2012. Anti-inflammatory and antioxidant activities of constituents isolated from Pueraria lobata roots. Arch. Pharm. Res. 35(5): 823-837. doi:10.1007/s12272-012-0508-x. PMID:22644850.
12. Kataoka, H., Kume, N., Miyamoto, S., Minami, M., Moriwaki, H., Murase, T., et al. 1999. Expression of lectinlike oxidized low-density lipoprotein receptor-1 in human atherosclerotic lesions. Circulation, 99(24): 3110-3117. doi:10.1161/01. CIR.99.24.3110. PMID:10377073.
13. Kataoka, H., Kume, N., Miyamoto, S., Minami, M., Morimoto, M., and Hayashida, K. 2001. Oxidized LDL modulates Bax/Bcl-2 through the lectin-like oxLDL receptor-1 in vascular smooth muscle cells. Arterioscler. Thromb. Vasc. Biol. 21: 955-960. doi:10.1161/01.ATV.21.6.955. PMID:11397703.
14. Keaney, J.F., Guo, Y., Cunninghan, D., Shwaery, G.T., Xu, A., and Vita, J.A. 1996. Vascular incorporation of alpha-tocopherol prevents endothelial dysfunction due to oxidized LDL by inhibiting protein kinase C stimulation. J. Clin. Invest. 98: 386-394. doi:10.1172/JCI118804. PMID:8755649.
15. Knowles, J.W., Reddick, R.L., Jennette, J.C., Shesely, E.G., Smithies, O., and Maeda, N. 2000. Enhanced atherosclerosis and kidney dysfunction in eNOS(-/-) Apoe(-/-) mice are ameliorated by enalapril treatment. J. Clin. Invest. 105(4): 451-458. doi:10.1172/JCI8376. PMID:10683374.
16. Kota, V., Ramana, A.B., and Satish, K.S. 2004. Aldose reductase regulates TNF- induced cell signaling and apoptosis in vascular endothelial cells. FEBS Lett. 570: 189-194. doi:10.1016/j.febslet.2004.06.046. PMID:15251463.
17. Kusuhara, M., Chait, A., Cader, A., and Berk, B.C. 1997. Oxidized LDL stimulates mitogen-activated protein kinases in smooth muscle cells and macrophages. Arterioscler. Thromb. Vasc. Biol. 17: 141-148. doi:10.1161/01.ATV.17.1.141. PMID: 9012649.
18. Lee, W.J., Ou, H.C., Hsu, W.C., Chou, M.M., Tseng, J.J., Hsu, S.L., et al. 2010. Ellagic acid inhibits oxidized LDL-mediated LOX-1 expression, ROS generation, and inflammation in human endothelial cells. J. Vasc. Surg. 52(5): 1290-1300. doi:10.1016/j.jvs.2010.04.085. PMID:20692795.
19. Li, D.Y., and Mehta, J.L. 2000. Upregulation of endothelial receptor for oxidized LDL (LOX-1) by oxidized LDL and implications in apoptosis of human coronary artery endothelial cells: evidence from use of antisense LOX-1 mRNA and chemical inhibitors. Arterioscler. Thromb. Vasc. Biol. 20: 1116-1122. doi:10. 1161/01.ATV.20.4.1116. PMID:10764682.
20. Li, D.Y., Yang, B.C., and Mehta, J.L. 1999. Ox-LDL induces apoptosis in human coronary artery endothelial cells: role of PKC, PTK, bcl-2, and Fas. Am. J. Physiol. 275: H568-H576. PMID:9683446.
21. Li, D.Y., Chen, H.J., and Mehta, J.L. 2001. Statins inhibit oxidized-LDL-mediated LOX-1 expression, uptake of oxidized-LDL and reduction in PKB phosphorylation. Cardiovasc. Res. 52: 130-135. doi:10.1016/S0008-6363(01)00371-6. PMID:11557241.
22. Li, D., Chen, H., Romeo, F., Sawamura, T., Saldeen, T., and Mehta, J.L. 2002. Statins modulate oxidized low-density lipoprotein-mediated adhesion molecule expression in human coronary artery endothelial cells: role of LOX-1. J. Pharmacol. Exp. Ther. 302: 601-605. doi:10.1124/jpet.102.034959. PMID:12130721.
23. Livak, K.J., and Schmittgen, T.D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(- C(T)) method. Methods, 25: 402-408. doi:10.1006/meth.2001.1262. PMID:11846609.
24. Mehta, J.L., and Li, D.Y. 2002. Identification, regulation and function of LOX-1, a novel receptor for ox-LDL. J. Am. Coll. Cardiol. 39: 1429-1435. doi:10.1016/S0735-1097(02)01803-X. PMID:11985903.
25. Mehta, J.L., Sanada, N., Hu, C.P., Chen, J., Dandapat, A., Sugawara, F., et al. 2007. Deletion of LOX-1 reduces atherogenesis in LDLR knockout mice fed high cholesterol diet. Circ. Res. 100: 1634-1642. doi:10.1161/CIRCRESAHA.107. 149724. PMID:17478727.
26. Meng, X.H., Ni, C., Zhu, L., and Shen, Y.L. 2009. Puerarin protects against high glucose-induced acute vascular dysfunction: Role of heme oxygenase-1 in rat thoracic aorta. Vascul. Pharmacol. 50: 110-115. doi:10.1016/j.vph.2008.11.003. PMID:19073281.
27. Oemar, B.S., Tschudi, M.R., Godoy, N., Brovkovich, V., Malinski, T., and Luscher, T.F. 1998. Reduced endothelial nitric oxide synthase expression and production in human atherosclerosis. Circulation, 97: 2494-2498. doi:10.1161/01.CIR.97.25.2494. PMID:9657467.
28. Ou, H.C., Song, T.Y., Yeh, Y.C., Huang, C.Y., Yang, S.F., Chiu, T.H., et al. 2010. EGCG protects against oxidized LDL-induced endothelial dysfunction by inhibiting LOX-1-mediated signaling. J. Appl. Phys. 108(6): 1745-1756. doi:10. 1152/japplphysiol.00879.2009. PMID:20203069.
29. Robbesyn, F., Salvayre, R., and Negre-Salvayre, A. 2004. Dual role of oxidized LDL on the NF-kappaB signaling pathway. Free Radic. Res. 38(6): 541-551. doi:10. 1080/10715760410001665244. PMID:15346645.
30. Romano, S., Mallardo, M., and Romano, M.F. 2011. FKBP51 and NF-κB regulatory pathway in cancer. Curr. Opin. Pharmacol. 11: 288-293. doi:10.1016/j.coph.2011.04.011. PMID:21565553.
31. Rubanye, G.M., and Ho, H.E. 1991. Cytoprotective function of nitric oxide: inactivation of superoxide radicals produced by human leukocytes. Biochem. Biophys. Res. Commun. 181: 1392-1397. doi:10.1016/0006-291X(91)92093-Y. PMID:1662497.
32. Rus, H.G., Vlaicu, R., and Niculescu, F. 1996. Interleukin-6 and interleukin-8 protein and gene expression in human arterial atherosclerotic wall. Atherosclerosis, 127: 263-271. doi:10.1016/S0021-9150(96)05968-0. PMID:9125317.
33. Sawamura, T., Kume, N., Aoyama, T., Moriwaki, H., Hoshikawa, H., and Aiba, Y. 1997. An endothelial receptor for oxidized low-density lipoprotein. Nature, 386: 73-77. doi:10.1038/386073a0. PMID:9052782.
34. Sevil, K., Sivakkanan, L., Beatrice, M., Tamas, R., Eniko, B., Kristof, H., et al. 2004. Upregulation of lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) in endothelial cells by nitric oxide deficiency. Am. J. Physiol. Renal. Physiol. 287: F25-F32. doi:10.1152/ajprenal.00449.2003. PMID:15010359.
35. Smirnova, I.V., Kajstura, M., Sawamura, T., and Goligorsky, M.S. 2004. Asymmetric dimethylarginine upregulates LOX-1 in activated macrophages: role in foam cell formation. Am. J. Physiol. Heart Circ. Physiol. 287(2): H782-H790. doi:10.1152/ajpheart.00822.2003. PMID:15016631.
36. Urban, M.B., Schreck, R., and Baeuerle, P.A. 1991. NF-kappaB contacts DNA by a heterodimer of the p50 and p65 subunit. EMBO J. 10: 1817-1825. PMID: 2050119.
37. Vermeulen, L., De Wilde, G., Notebaert, S., Vanden Berghe, W., and Haegeman, G. 2002. Regulation of the transcriptional activity of the nuclear factor-kappaB p65 subunit. Biochem. Pharmacol. 64: 963-970. doi:10.1016/S0006-2952(02)01161-9. PMID:12213593.
38. Widlansy, M.E., Gokce, N., Keaney, J.F., and Vita, J.A. 2003. The clinical implications of endothelial dysfunction. J. Am. Coll. Cardiol. 42: 1149-1160. doi:10. 1016/S0735-1097(03)00994-X. PMID:14522472.
39. Witztum, J.L., and Steinberg, D. 1991. Role of oxidized low-density lipoprotein in atherogenesis. J. Clin. Invest. 88: 1785-1792. doi:10.1172/JCI115499. PMID: 1752940.
40. Yan, L.P., Chan, S.W., Chan, S.C.C., and Chen, S.L. 2006. Puerarin decreases serum total cholesterol and enhances thoracic aorta endothelial nitric oxide synthase expression in diet-induced hypercholesterolemic rats. Life Sci. 79: 324-330. doi:10.1016/j.lfs.2006.01.016. PMID:16472823.
41. Yang, X., Hu, W., Zhang, Q., Wang, Y., and Sun, L. 2010. Puerarin inhibits C-reactive protein expression via suppression of nuclear factor κB activation in lipopolysaccharide-induced peripheral blood mononuclear cells of patients with stable angina pectoris. Basic Clin. Pharmacol. 107: 637-642. doi: 10.1111/j.1742-7843.2010.00548.x.
42. Yoshida, H., Kondratenko, N., Green, S., Steinberg, D., and Quehenberger, O. 1998. Identification of the lectin-like receptor for oxidized lowdensity lipoprotein in human macrophages and its potential role as a scavenger receptor. Biochem. J. 334: 9-13. PMID:9693095.
43. Zhao, J., Qi, R., Li, R., Wu, W., Gao, X., Bao, L., and Lu, S. 2008. Protective effects of aspirin against oxidized LDL-induced inflammatory protein expression in human endothelial cells. J. Cardiovasc. Pharmacol. 51: 32-37. doi:10.1097/FJC. 0b013e318159ebaf. PMID:18209566.