Tandem dyes: Stability in cocktails and compensation considerations

Cytometry Part B - Clinical Cytometry

Volumn 86, Issue 3, 2014, Pages 164-174

  Johansson, Ulrika ^a   MacEy, Marion ^b  

^a BRISTOL ROYAL INFIRMARY   (United Kingdom)

^b Immunophenotyping Pathology and Pharmacy Building 80   (United Kingdom)

Author keywords

compensation matrix; monoclonal antibody cocktails; spectral spillover; tandem conjugates

Indexed keywords

ANTIBODY CONJUGATE; ANTISERUM; DYE; FLUOROCHROME; MONOCLONAL ANTIBODY; CARBOCYANINE; FLUORESCENT DYE; INDOTRICARBOCYANINE; PHYCOERYTHRIN;

ARTICLE; BLOOD; BONE MARROW CELL; CELL POPULATION; DATA ANALYSIS; FLOW CYTOMETRY; FLUORESCENCE; FREEZE DRYING; MONOCYTE; PRIORITY JOURNAL; CHEMISTRY; COLOR; CRYOPRESERVATION; CYTOLOGY; DIAGNOSTIC USE; HUMAN; SIGNAL NOISE RATIO; STANDARDS;

ANTIBODIES, MONOCLONAL; BONE MARROW CELLS; CARBOCYANINES; COLOR; CRYOPRESERVATION; FLOW CYTOMETRY; FLUORESCENT DYES; FREEZE DRYING; HUMANS; IMMUNOCONJUGATES; PHYCOERYTHRIN; SIGNAL-TO-NOISE RATIO;

EID: 84898548094     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.21154     Document Type: Article

References (16)

1. Biancotto A, Fuchs JC, Williams A, Dagur PK, McCoy JP Jr,. High dimensional flow cytometry for comprehensive leukocyte immunophenotyping (CLIP) in translational research. J Immunol Methods 2011; 363: 245-261.
2. Rawstron AC, Bottcher S, Letestu R, Villamor N, Fazi C, Kartsios H, de Tute RM, Shingles J, Ritgen M, Moreno C, et al. Improving efficiency and sensitivity: European Research Initiative in CLL (ERIC) update on the international harmonised approach for flow cytometric residual disease monitoring in CLL. Leukemia 2013; 27: 142-149.
3. Forman MA, Gupta RK,. Tandem dyes for flow cytometry: Can we overcome quality concerns? MLO Med Lab Obs 2007; 39: 24, 26.
4. Stelzer GT, Marti G, Hurley A, McCoy P Jr, Lovett EJ, Schwartz A,. U.S.-Canadian Consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: Standardization and validation of laboratory procedures. Cytometry 1997; 30: 214-230.
5. Stewart CC, Stewart SJ,. Four color compensation. Cytometry 1999; 38: 161-175.
6. Baumgarth N, Roederer M,. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000; 243: 77-97.
7. Hulspas R, Dombkowski D, Preffer F, Douglas D, Kildew-Shah B, Gilbert J,. Flow cytometry and the stability of phycoerythrin-tandem dye conjugates. Cytometry A 2009; 75A: 966-972.
8. Wood B,. 9-color and 10-color flow cytometry in the clinical laboratory. Arch Pathol Lab Med 2006; 130: 680-690.
9. Matarraz S, Lopez A, Barrena S, Fernandez C, Jensen E, Flores-Montero J, Rasillo A, Sayagues JM, Sanchez ML, Barcena P, et al. Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease: A pilot study on a series of 56 patients. Cytometry B Clin Cytom 2010; 78B: 154-168.
10. van Lochem EG, van der Velden VH, Wind HK, te Marvelde JG, Westerdaal NA, van Dongen JJ,. Immunophenotypic differentiation patterns of normal hematopoiesis in human bone marrow: Reference patterns for age-related changes and disease-induced shifts. Cytometry B Clin Cytom 2004; 60B: 1-13.
11. Wood BL,. Flow cytometric diagnosis of myelodysplasia and myeloproliferative disorders. J Biol Regul Homeost Agents 2004; 18: 141-145.
12. Wells DA, Benesch M, Loken MR, Vallejo C, Myerson D, Leisenring WM, Deeg HJ,. Myeloid and monocytic dyspoiesis as determined by flow cytometric scoring in myelodysplastic syndrome correlates with the IPSS and with outcome after hematopoietic stem cell transplantation. Blood 2003; 102: 394-403.
13. Arnoulet C, Bene MC, Durrieu F, Feuillard J, Fossat C, Husson B, Jouault H, Maynadie M, Lacombe F,. Four- and five-color flow cytometry analysis of leukocyte differentiation pathways in normal bone marrow: A reference document based on a systematic approach by the GTLLF and GEIL. Cytometry B Clin Cytom 2010; 78B: 4-10.
14. Le Roy C, Varin-Blank N, Ajchenbaum-Cymbalista F, Letestu R,. Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism. Cytometry A 2009; 75A: 882-890.
15. Chattopadhyay PK, Gaylord B, Palmer A, Jiang N, Raven MA, Lewis G, Reuter MA, Nur-ur Rahman AK, Price DA, Betts MR, et al. Brilliant violet fluorophores: A new class of ultrabright fluorescent compounds for immunofluorescence experiments. Cytometry A 2012; 81A: 456-466.
16. Westers TM, Ireland R, Kern W, Alhan C, Balleisen JS, Bettelheim P, Burbury K, Cullen M, Cutler JA, Della Porta MG, et al. Standardization of flow cytometry in myelodysplastic syndromes: A report from an international consortium and the European LeukemiaNet Working Group. Leukemia 2012; 26: 1730-1741.