A xeno-free culture method that enhances Wharton's jelly mesenchymal stromal cell culture efficiency over traditional animal serum-supplemented cultures

Cytotherapy

Volumn 16, Issue 5, 2014, Pages 683-691

  Julavijitphong, Suphakde ^a   Wichitwiengrat, Suparat ^a   Tirawanchai, Nednapis ^a   Ruangvutilert, Pornpimol ^a   Vantanasiri, Chanchai ^a   Phermthai, Tatsanee ^a  

^a MAHIDOL UNIVERSITY   (Thailand)

Author keywords

Animal free stem cell line; Cord blood serum; Mesenchymal stromal cells; Umbilical cord blood; Wharton's jelly stem cells; Xeno free stem cell

Indexed keywords

5' NUCLEOTIDASE; BETA1 INTEGRIN; CD34 ANTIGEN; CD45 ANTIGEN; ENDOGLIN; THY 1 ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; CELL AGING; CELL DIFFERENTIATION; CELL GROWTH; CELL LINEAGE; CELL PROLIFERATION; CELL STRAIN; COCULTURE; CONTROLLED STUDY; CRYOPRESERVATION; CULTURE MEDIUM; CULTURE TECHNIQUE; EMBRYO; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MESENCHYMAL STEM CELL; MOLECULAR STABILITY; PHENOTYPE; PRIORITY JOURNAL; STEM CELL CULTURE; UMBILICAL CORD BLOOD; WHARTON JELLY; XENO-FREE CULTURE; CELL LINE; CYTOLOGY; FETUS BLOOD; MESENCHYMAL STROMA CELL; PROCEDURES;

ANIMALIA; BOVINAE;

CELL DIFFERENTIATION; CELL LINE; COCULTURE TECHNIQUES; CULTURE TECHNIQUES; FETAL BLOOD; HUMANS; MESENCHYMAL STROMAL CELLS; WHARTON JELLY;

EID: 84897961079     PISSN: 14653249     EISSN: 14772566     Source Type: Journal    
DOI: 10.1016/j.jcyt.2013.07.012     Document Type: Article

References (23)

1. Mimeault M., Batra S.K. Concise review: recent advances on the significance of stem cells in tissue regeneration and cancer therapies. Stem Cells 2006, 24:2319-2345.
2. Pittenger M.F., Mackay A.M., Beck S.C., Jaiswal R.K., Douglas R., Mosca J.D., et al. Multilineage potential of adult human mesenchymal stem cells. Science 1999, 284:143-147.
3. Lakshmipathy U., Verfaillie C. Stem cell plasticity. Blood Rev 2005, 19:29-38.
4. Phermthai T., Odglun Y., Julavijitphong S., Titapant V., Chuenwattana P., Vantanasiri C., et al. Anovel method to derive amniotic fluid stem cells for therapeutic purposes. BMC Cell Biol 2010, 11:79.
5. Le Blanc K. Immunomodulatory effects of fetal and adult mesenchymal stem cells. Cytotherapy 2003, 5:485-489.
6. Caplan A.I., Dennis J.E. Mesenchymal stem cells as trophic mediators. JCell Biochem 2006, 98:1076-1084.
7. Bongso A., Fong C.Y., Gauthaman K. Taking stem cells to the clinic: major challenges. JCell Biochem 2008, 105:1352-1360.
8. Can A., Karahuseyinoglu S. Concise review: human umbilical cord stroma with regard to the source of fetus-derived stem cells. Stem Cells 2007, 25:2886-2895.
9. Yoo K.H., Jang I.K., Lee M.W., Kim H.E., Yang M.S., Eom Y., et al. Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues. Cell Immunol 2009, 259:150-156.
10. Weiss M.L., Anderson C., Medicetty S., Seshareddy K.B., Weiss R.J., VanderWerff I., et al. Immune properties of human umbilical cord Wharton's jelly-derived cells. Stem Cells 2008, 26:2865-2874.
11. Yang C.C., Shih Y.H., Ko M.H., Hsu S.Y., Cheng H., Fu Y.S. Transplantation of human umbilical mesenchymal stem cells from Wharton's jelly after complete transection of the rat spinal cord. PLoS One 2008, 3:e3336.
12. Koh S.H., Kim K.S., Choi M.R., Jung K.H., Park K.S., Chai Y.G., et al. Implantation of human umbilical cord-derived mesenchymal stem cells as a neuroprotective therapy for ischemic stroke in rats. Brain Res 2008, 1229:233-248.
13. Amit M., Margulets V., Segev H., Shariki K., Laevsky I., Coleman R., et al. Human feeder layers for human embryonic stem cells. Biol Reprod 2003, 68:2150-2156.
14. Venugopal P., Balasubramanian S., Majumdar A.S., Ta M. Isolation, characterization, and gene expression analysis of Wharton's jelly-derived mesenchymal stem cells under xeno-free culture conditions. Stem Cells Cloning: Advances Applications 2011, 4:39-50.
15. Hatlapatka T., Moretti P., Lavrentieva A., Hass R., Marquardt N., Jacobs R., et al. Optimization of culture conditions for the expansion of umbilical cord-derived mesenchymal stem or stromal cell-like cells using xeno-free culture conditions. Tissue Eng Part C Methods 2011, 17:485-493.
16. Bieback K., Hecker A., Schlechter T., Hofmann I., Brousos N., Redmer T., et al. Replicative aging and differentiation potential of human adipose tissue-derived mesenchymal stromal cells expanded in pooled human or fetal bovine serum. Cytotherapy 2012, 14:570-583.
17. Phermthai T., Odglun Y., Chuenwattana P., Julavijitphong S., Titapant V., Vantanasiri C. Successful derivation and characteristics of xeno-free mesenchymal stem cell lines from human amniotic fluid generated under allogenic cord blood serum supplementation. Tissue Eng Regen Med 2011, 8:216-223.
18. Broxmeyer H.E., Benninger L., Yip-Schneider M., Braun S.E. Commentary: a rapid proliferation assay for unknown co-stimulating factors in cord blood plasma possibly involved in enhancement of in vitro expansion and replating capacity of human hematopoietic stem/progenitor cells. Blood Cells 1994, 20:492-497.
19. Shetty P., Bharucha K., Tanavde V. Human umbilical cord blood serum can replace fetal bovine serum in the culture of mesenchymal stem cells. Cell Biol Int 2007, 31:293-298.
20. Phermthai T., Suksompong S., Tirawanchai N., Issaragrisil S., Julavijitphong S., Wichitwiengrat S., et al. Epigenetic analysis and suitability of amniotic fluid stem cells for research and therapeutic purposes. Stem Cells Dev 2013, 22:1-10.
21. Park Y.B., Kim Y.Y., Oh S.K., Chung S.G., Ku S.Y., Kim S.H., et al. Alterations of proliferative and differentiation potentials of human embryonic stem cells during long-term culture. Exp Mol Med 2008, 40:98-108.
22. Zaim M., Karaman S., Cetin G., Isik S. Donor age and long-term culture affect differentiation and proliferation of human bone marrow mesenchymal stem cells. Ann Hematol 2012, 91:1175-1186.
23. Safwani W.K., Makpol S., Sathapan S., Chua K.H. The impact of long-term in vitro expansion on the senescence-associated markers of human adipose-derived stem cells. Appl Biochem Biotechnol 2012, 166:2101-2113.