Bacillus thuringiensis subsp. Israelensis and Its dipteran-specific toxins

Toxins

Volumn 6, Issue 4, 2014, Pages 1222-1243

  Ben Dov, Eitan ^a  

^a ACHVA ACADEMIC COLLEGE   (Israel)

Author keywords

Biological control; Larvicidal crystal proteins; Mosquito borne diseases

Indexed keywords

BACILLUS THURINGIENSIS TOXIN; BACTERIAL TOXIN; CRY TOXIN; CRY11AA TOXIN; CRY4AA TOXIN; CRY4BA TOXIN; CYT2BA TOXIN; LARVICIDAL AGENT; UNCLASSIFIED DRUG; ANTIINFECTIVE AGENT; ANTINEOPLASTIC AGENT; BACTERIAL PROTEIN; BIOLOGICAL CONTROL AGENT; ENDOTOXIN; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; INSECTICIDE;

ANTIBACTERIAL ACTIVITY; ANTINEOPLASTIC ACTIVITY; BACILLUS THURINGIENSIS; BINDING AFFINITY; BIOLOGICAL PEST CONTROL; CULEX PIPIENS; DIPTERA; MOSQUITO; NONHUMAN; PROTEIN INTERACTION; REVIEW; SIMULIIDAE; TETRAHYMENA PYRIFORMIS; TOXIN STRUCTURE; ANIMAL; BIOLOGICAL CONTROL AGENT; DRUG EFFECTS; EMBRYOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSECTICIDE RESISTANCE; LARVA; METABOLISM; PROCEDURES;

ANIMALS; ANTI-BACTERIAL AGENTS; ANTINEOPLASTIC AGENTS; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BIOLOGICAL CONTROL AGENTS; CULICIDAE; ENDOTOXINS; HEMOLYSIN PROTEINS; INSECTICIDE RESISTANCE; INSECTICIDES; LARVA; MOSQUITO CONTROL; SIMULIIDAE;

EID: 84897551966     PISSN: 20726651     EISSN: None     Source Type: Journal    
DOI: 10.3390/toxins6041222     Document Type: Review

References (183)

1. Service, M.W. Medical Entomology for Students, 3rd ed.; Cambridge University Press: Cambridge, UK, 2004.
2. Sinka, M.E.; Bangs, M.J.; Manguin, S.; Rubio-Palis, Y.; Chareonviriyaphap, T.; Coetzee, M.; Mbogo, C.M.; Hemingway, J.; Patil, A.; Temperley, W.H. A global map of dominant malaria vectors. Parasit Vectors 2012, 5, doi:10.1186/1756-3305-5-69.
3. Gyapong, J.O.; Twum-Danso, N.A.Y. Editorial: Global elimination of lymphatic filariasis: Fact or fantasy? Trop. Med. Int. Health 2006, 11, 125-128.
4. Reisen, W.K. Landscape epidemiology of vector-borne diseases. Annu. Rev. Entomol. 2010, 55, 461-483.
5. World Health Organization. World Malaria Report 2012. WHO: Geneva, Switzland, 2012.
6. Weill, M.; Lutfalla, G.; Mogensen, K.; Chandre, F.; Berthomieu, A.; Berticat, C.; Pasteur, N.; Philips, A.; Fort, P.; Raymond, M. Insecticide resistance in mosquito vectors. Nature 2003, 423, 136-137.
7. Federici, B.A.; Park, H.W.; Bideshi, D.K. Overview of the basic biology of Bacillus thuringiensis with emphasis on genetic engineering of bacterial larvicides for mosquito control. Open Toxinol. J. 2010, 3, 83-100.
8. Fillinger, U.; Lindsay, S.W. Suppression of exposure to malaria vectors by an order of magnitude using microbial larvicides in rural Kenya. Trop. Med. Int. Health 2006, 11, 1629-1642.
9. Margalith, Y.; Ben-Dov, E. Biological control by Bacillus thuringiensis subsp. israelensis. In Insect Pest Management: Techniques for Environmental Protection; Rechcigl, J.E., Rechcigl, N.A., Eds.; CRC Press: Boca Raton, FL, USA, 2000; pp. 243-301.
10. Becker, N.; Ludwig, M. Investigations on possible resistance in Aedes vexans field populations after a 10-years application of Bacillus thuringiensis israelensis. J. Am. Mosq. Control Assoc. 1993, 9, 221-224.
11. Kamgang, B.; Marcombe, S.; Chandre, F.; Nchoutpouen, E.; Nwane, P.; Etang, J.; Corbel, V.; Paupy, C. Insecticide susceptibility of Aedes aegypti and Aedes albopictus in Central Africa. Parasit Vectors 2011, 4, doi:10.1186/1756-3305-4-79.
12. Loke, S.R.; Andy-Tan, W.A.; Benjamin, S.; Lee, H.L.; Sofian-Azirun, M. Susceptibility of field-collected Aedes aegypti (L.) (Diptera: Culicidae) to Bacillus thuringiensis israelensis and temephos. Trop. Biomed. 2010, 27, 493-503.
13. Tetraeu, G.; Stalinski, R.; David, J.P.; Despres, L. Monitoring resistance to Bacillus thuringiensis israelensis (Bti) in the field by performing bioassays with each Cry toxin superlatively. Mem. Inst. Oswaldo Cruz 2013, 108, 894-900.
14. Vasquez, M.I.; Violaris, M.; Hadjivassilis, A.; Wirth, M.C. Susceptibility of Culex pipiens (Diptera: Culicidae) field populations in Cyprus to conventional organic insecticides, Bacillus thuringiensis subsp. israelensis and methoprene. J. Med. Entomol. 2009, 46, 881-887.
15. Wirth, M.C. Mosquito resistance to bacterial larvicidal toxins. Open Toxinol. J. 2010, 3, 126-140.
16. Boyer, S.; Paris, M.; Jego, S.; Lemperiere, G.; Ravanel, P. Influence of insecticide Bacillus thuringiensis subsp. israelensis treatments on resistance and enzyme activities in Aedes rusticus larvae (Diptera: Culicidae). Biol. Control 2012, 62, 75-81.
17. Paul, A.; Harrington, L.C.; Zhang, L.; Scott, J.G. Insecticide resistance in Culex pipiens from New York. J. Am. Mosq. Control Assoc. 2005, 21, 305-309.
18. Zhang, H.Y.; Yang, C.J.; Huang, J.Y.; Lu, L. Susceptibility of field populations of Anopheles sinensis (Diptera: Culicidae) to Bacillus thuringiensis subsp. israelensis. Biocontrol Sci. Technol. 2004, 14, 321-325.
19. Otieno-Ayayo, Z.N.; Zaritsky, A.; Wirth, M.C.; Manasherob, R.; Khasdan, V.; Cahan, R.; Ben-Dov, E. Variations in the mosquito larvicidal activities of toxins from Bacillus thuringiensis ssp. israelensis. Environ. Microbial. 2008, 10, 2191-2199.
20. Crickmore, N.; Bone, E.J.; Williams, J.A.; Ellar, D.J. Contribution of the individual components of the δ-endotoxin crystal to the mosquitocidal activity of Bacillus thuringiensis subsp. israelensis. FEMS Microbiol. Lett. 1995, 131, 249-254.
21. Poncet, S.; Delecluse, A.; Klier, A.; Rapoport, G. Evaluation of synergistic interactions among the CryIVA, CryIVB, and CryIVD toxic components of B. thuringiensis subsp. israelensis crystals. J. Invertebr. Pathol. 1995, 66, 131-135.
22. Wirth, M.C.; Park, H.-W.; Walton, W.E.; Federici, B.A. Cyt1A of Bacillus thuringiensis delays evolution of resistance to Cry11A in the mosquito Culex quinquefasciatus. Appl. Environ. Microbial. 2005, 71, 185-189.
23. Pereira, E.; Teles, B.; Martins, E.; Pral L.; Santos, A.; Ramos, F.; Berry, C.; Monnerat, R. Comparative toxicity of Bacillus thuringiensis berliner strains to larvae of simuliidae (Insecta: Diptera). Bt Res. 2013, 4, 8-13.
24. Ali, A. A concise review of chironomid midges (Diptera: Chironomidae) as pests and their management. J. Vector Ecol. 1996, 21, 105-121.
25. Kondo, S.; Ohba, M.; Ishii, T. Comparative susceptibility of chironomid larvae (Dipt., Chironomidae) to Bacillus thuringiensis serovar israelensis with special reference to altered susceptibility due to food difference. J. Appl. Entomol. 1995, 119, 123-125.
26. Saraswathi, A.; Ranganathan, L.S. Larvicidal effect of Bacillus thuringiensis var. israelensis on Tabanus triceps (Thunberg) (Diptera: Tabanidae). Indian J. Exp. Biol. 1996, 34, 1155-1157.
27. Robacker, D.C.; Martinez, A.J.; Garcia, J.A.; Diaz, M.; Romero, C. Toxicity of Bacillus thuringiensis to Mexican fruit fly (Diptera: Tephritidae). J. Econ. Entomol. 1996, 89, 104-110.
28. Vidal-Quist, J.C.; Castañera, P.; González-Cabrera, J. Cyt1Aa protein from Bacillus thuringiensis (Berliner) serovar israelensis is active against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann). Pest Manag. Sci. 2010, 66, 949-955.
29. Oestergaard, J.; Belau, C.; Strauch, O.; Ester, A.; van Rozen, K.; Ehlers, R.U. Biological control of Tipula paludosa (Diptera: Nematocera) using entomopathogenic nematodes (Steinernema spp.) and Bacillus thuringiensis subsp. israelensis. Biol. Control 2006, 39, 525-531.
30. Harris, M.A.; Oetting, R.D.; Gardner, W.A. Use of entomopathogenic nematodes and a new monitoring technique for control of fungus gnats, Bradysia coprophila (Diptera: Sciaridae), in floriculture. Biol. Control 1995, 5, 412-418.
31. Taylor, M.D.; Willey, R.D.; Noblet, R. A 24-h potato-based toxicity test for evaluating Bacillus thuringiensis var. israelensis (H-14) against darkwinged fungus gnat Bradysia impatiens Johannsen (Diptera: Sciaridae) larvae. Int. J. Pest Manag. 2007, 53, 77-81.
32. Nambiar, P.T.C.; Ma, S.W.; Iyer, V.N. Limiting an insect infestation of nitrogen-fixing root nodules of the pigeon pea (Cajanus cajan) by engineering the expression of an entomocidal gene in its root nodules. Appl. Environ. Microbiol. 1990, 56, 2866-2869.
33. Porcar, M.; Grenier, A.M.; Federici, B.; Rahbé, Y. Effects of Bacillus thuringiensis δ-endotoxins on the Pea aphid (Acyrthosiphon pisum). Appl. Environ. Microbiol. 2009, 75, 4897-4900.
34. Walters, F.S.; English, L.H. Toxicity of Bacillus thuringiensis δ-endotoxins toward the potato aphid in an artificial diet bioassay. Entomol. Exp. Appl. 1995, 77, 211-216.
35. Monnerat, R.; Martins, E.; Praça, L.; Dumas, V.; Berry, C. Activity of a Brazilian strain of Bacillus thuringiensis israelensis against the cotton boll weevil Anthonomus grandis Boheman (Coleoptera: Tenebrionidae). Neotrop. Entomol. 2012, 41, 62-67.
36. Federici, B.A.; Bauer, L.S. Cyt1Aa protein of Bacillus thuringiensis is toxic to the cottonwood leaf beetle, Chrysomela scripta, and suppresses high levels of resistance to Cry3Aa. Appl. Environ. Microbiol. 1998, 64, 4368-4371.
37. De Souza, J.D.A., Jr.; Jain, S.; de Oliveira, C.M.F.; Ayres, C.F.; Lucena, W.A. Toxicity of a Bacillus thuringiensis israelensis-like strain against Spodoptera frugiperda. BioControl 2009, 54, 467-473.
38. Sayyed, A.H.; Crickmore, N.; Wright, D.J. Cyt1Aa from Bacillus thuringiensis subsp. israelensis is toxic to the diamondback moth, Plutella xylostella, and synergizes the activity of Cry1Ac towards a resistant strain. Appl. Environ. Microbiol. 2001, 67, 5859-5861.
39. Sharma, R.D. Bacillus thuringiensis: A biocontrol agent of Meloidogyne incognita on barley. Nematol. Brasileira 1994, 18, 79-84.
40. Horak, P.; Weiser, J.; Mikes, L.; Kolarova, L. The effect of Bacillus thuringiensis M-exotoxin on trematode cercariae. J. Invertebr. Pathol. 1996, 68, 41-49.
41. Goldberg, L.J.; Margalit, J. A bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univittatus, Aedes aegypti and Culex pipiens. Mosq. News 1977, 37, 355-358.
42. Boisvert, M.; Boisvert, J. Persistence of toxic activity and recycling of Bacillus thuringiensis var. israelensis in cold water: Field experiments using diffusion chambers in a pond. Biocontrol Sci. Technol. 1999, 9, 507-522.
43. Tilquin, M.; Paris, M.; Reynaud, S.; Despres, L.; Ravanel, P.; Geremia, R.A.; Gury, J. Long lasting persistence of Bacillus thuringiensis subsp. israelensis (Bti) in mosquito natural habitats. PLoS ONE 2008, 3, e3432.
44. Tetreau, G.; Alessi, M.; Veyrenc, S.; Périgon, S.; David, J.P.; Reynaud, S.; Després, L. Fate of Bacillus thuringiensis subsp. israelensis in the field: Evidence for spore recycling and differential persistence of toxins in leaf litter. Appl. Environ. Microbiol. 2012, 78, 8362-8367.
45. Aly, C.; Mulla, M.S.; Federici, B.A. Sporulation and toxin production by Bacillus thuringiensis var. israelensis in cadavers of mosquito larvae (Diptera: Culicidae). J. Invertebr. Pathol. 1985, 46, 251-258.
46. Zaritsky, A.; Khawaled, K. Toxicity in carcasses of Bacillus thuringiensis var. israelensis-killed Aedes aegypti larvae against scavenging larvae: Implications to bioassay. J. Am. Mosq. Control Assoc. 1986, 2, 555-559.
47. Khawaled, K.; Barak, Z.; Zaritsky, A. Feeding behavior of Aedes aegypti larvae and toxicity of dispersed and of naturally encapsulated Bacillus thuringiensis var. israelensis. J. Invertebr. Pathol. 1988, 52, 419-426
48. Khawaled, K.; Ben-Dov, E.; Zaritsky, A.; Barak, Z. The fate of Bacillus thuringiensis var. israelensis in B. thuringiensis var. israelensis-killed pupae. J. Invertebr. Pathol. 1990, 56, 312-316.
49. Manasherob, R.; Ben-Dov, E.; Zaritsky, A.; Barak, Z. Germination, growth, and sporulation of Bacillus thuringiensis subsp. israelensis in excreted food vacuoles of the protozoan Tetrahymena pyriformis. Appl. Environ. Microbiol. 1998, 64, 1750-1758.
50. Ben-Dov, E.; Zalkinder, V.; Shagan, T.; Barak, Z.; Zaritsky, A. Spores of Bacillus thuringiensis var. israelensis as tracers for ingestion rates by Tetrahymena pyriformis. J. Invertebr. Pathol. 1994, 63, 220-222.
51. Carlton, B.C.; Gonzalez, J.M., Jr. Plasmids and delta-endotoxin production in different subspecies of Bacillus thuringiensis. In Molecular Biology of Microbial Differentiation; Hoch, J.A., Setlow, P., Eds.; American Society for Microbiology: Washington, DC, USA, 1985; pp. 246-252.
52. Sekar, V. Genetics of Bacillus thuringiensis israelensis. In Bacterial Control of Mosquitoes and Black Flies; de Barjac, H., Sutherland, D.J., Eds.; Rutgers University Press: New Brunswick, NJ, USA, 1990; pp. 66-77.
53. Ben-Dov, E.; Einav, M.; Peleg, N.; Boussiba, S.; Zaritsky, A. Restriction map of the 125-kilobase of Bacillus thuringiensis subsp. israelensis carrying the genes that encode delta-endotoxins active against mosquito larvae. Appl. Environ. Microbiol. 1996, 62, 3140-3145.
54. Ben-Dov, E.; Nissan, G.; Peleg, N.; Manasherob, R.; Boussiba, S.; Zaritsky, A. Refined, circular restriction map of the Bacillus thuringiensis subsp. israelensis plasmid carrying the mosquito larvicidal genes. Plasmid 1999, 42, 186-191.
55. Berry, C.; O'Neil, S.; Ben-Dov, E.; Jones, A.F.; Murphy, L.; Quail, M.A.; Holden, M.T.G.; Harris, D.; Zaritsky, A.; Parkhill, J. Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 2002, 68, 5082-5095.
56. Guerchicoff, A.; Ugalde, R.A.; Rubinstein, C.P. Identification and characterization of a previously undescribed cyt gene in Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 1997, 63, 2716-2721.
57. Lee, S.G.; Eckblad, W.; Bulla, A., Jr. Diversity of protein inclusion bodies and identification of mosquitocidal protein in Bacillus thuringiensis subsp. israelensis. Biochem. Biophys. Res. Commun. 1985, 126, 953-960.
58. Thorne, L.; Garguno, F.; Thompson, T.; Decker, D.; Zounes, M.; Wild, M.; Walfieldand, A.M.; Pollock, T.J. Structural similarity between the Lepidoptera-and Diptera-specific isecticidal endotoxins genes of Bacillus thuringiensis subsp. kurstaki and israelensis. J. Bacteriol. 1986, 166, 801-811.
59. Bietlot, H.P.L.; Vishnubhatla, I.; Carey, P.R.; Pozsgay, M.; Kaplan, H. Characterization of the cysteine residues and disulfide linkages in the protein crystal of Bacillus thuringiensis. Biochem. J. 1990, 267, 309-315.
60. Couche, G.A.; Pfannenstiel, M.A.; Nickerson, K.W. Structural disulfide bonds in the Bacillus thuringiensis subsp. israelensis protein crystal. J. Bacteriol. 1987, 169, 3281-3288.
61. Adams, L.F.; Visick, J.E.; Whiteley. H.R. A 20-kilodalton protein is required for efficient production of the Bacillus thuringiensis subsp. israelensis 27-kilodalton crystal protein in Escherichia coli. J. Bacteriol. 1989, 171, 521-530.
62. Visick, J.E.; Whiteley, H.R. Effect of a 20-kilodalton protein from Bacillus thuringiensis subsp. israelensis on production of the CytA protein by Escherichia coli. J. Bacteriol. 1991, 173, 1748-1756.
63. Xu, Y.; Nagai, M.; Bagdasarian, M.; Smith, T.W.; Walker, E.D. Expression of the p20 gene from Bacillus thuringiensis H-14 increases Cry11A toxin production and enhances mosquito-larvicidal activity in recombinant gram-negative bacteria. Appl. Environ. Microbiol. 2001, 67, 3010-3015.
64. Agaisse, H.; Lereclus, D. How does Bacillus thuringiensis produce so much insecticidal crystal protein? J. Bacteriol. 1995, 177, 6027-6032.
65. Dervyn, E.; Poncet, S.; Klier, A.; Rapoport, G. Transcriptional regulation of the cryIVD gene operon from Bacillus thuringiensis subsp. israelensis. J. Bacteriol. 1995, 177, 2283-2291.
66. Ben-Dov, E.; Boussiba, S.; Zaritsky, A. Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis. J. Bacteriol. 1995, 177, 2851-2857.
67. McLean, K.M.; Whiteley, H.R. Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis. J. Bacteriol. 1987, 169, 1017-1023.
68. Wu, D.; Federici, B.A. A 20-kilodalton protein preserves cell viability and promotes CytA crystal formation during sporulation in Bacillus thuringiensis. J. Bacteriol. 1993, 175, 5276-5280.
69. Wu, D.; Federici, B.A. Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein. Appl. Microbiol. Biotechnol. 1995, 45, 697-702.
70. Abdullah, M.A.F.; Alzate, O.; Mohammad, M.; McNall, R.J.; Adang, M.J.; Dean, D.H. Introduction of Culex toxicity into Bacillus thuringiensis Cry4Ba by protein engineering. Appl. Environ. Microbiol. 2003, 69, 5343-5353.
71. Chen, J.; Likitvivatanavong, S.; Aimanova, K.G.; Gill, S.S. A 104 kDa Aedes aegypti aminopeptidase N is a putative receptor for the Cry11Aa toxin from Bacillus thuringiensis subsp. israelensis. Insect Biochem. Molec. Biol. 2013, 43, 1201-1208.
72. Feldmann, F.; Dullemans, A.; Waalwijk, C. Binding of the CryIVD toxin of Bacillus thuringiensis subsp. israelensis to larval dipteran midgut proteins. Appl. Environ. Microbiol. 1995, 61, 2601-2605.
73. Fernandez, L.E.; Aimanova, K.G.; Gill, S.S.; Bravo, A.; Soberón, M. A GPI-anchored alkaline phosphatase is a functional midgut receptor of Cry11Aa toxin in Aedes aegypti larvae. Biochem. J. 2006, 394, 77-84.
74. Pigott, C.R.; Ellar, D.J. Role of receptors in Bacillus thuringiensis crystal toxin activity. Microbiol. Molec. Biol. Rev. 2007, 71, 255-281.
75. Yamagiwa, M.; Kamauchi, S.; Okegawa, T.; Esaki, M.; Otake, K.; Amachi, T.; Komano, T.; Sakai, H. Binding properties of Bacillus thuringiensis Cry4A toxin to the apical microvilli of larval midgut of Culex pipiens. Biosci. Biotechnol. Biochem. 2001, 11, 2419-2427.
76. Thomas, W.E.; Ellar, D.J. Mechanism of action of Bacillus thuringiensis var. israelensis insecticidal δ-endotoxin. FEBS Lett. 1983, 154, 362-368.
77. Cantón, P.E.; López-Díaz, J.A.; Gill, S.S.; Bravo, A.; Soberón, M. Membrane binding and oligomer membrane insertion are necessary but insufficient for Bacillus thuringiensis Cyt1Aa toxicity. Peptides 2013, in press.
78. Angsuthanasombat, C.; Crickmore, N.; Ellar, D.J. Comparison of Bacillus thuringiensis subsp. israelensis CryIVA and CryIVB cloned toxins reveals synergism in vivo. FEMS Microbiol. Lett. 1992, 94, 63-68.
79. Beltrão, H.B.M.; Silva-Filha, M.H.N.L. Interaction of Bacillus thuringiensis svar. israelensis Cry toxins with binding sites from Aedes aegypti (Diptera: Culicidae) larvae midgut. FEMS Microbiol. Lett. 2007, 266, 163-169.
80. Sen, K.; Honda, G.; Koyama, N.; Nishida, M.; Neki, A.; Sakai, H.; Himeno, M.; Komano, T. Cloning and nucleotide sequences of the two 130 kDa insecticidal protein genes of Bacillus thuringiensis var. israelensis. Agric. Biol. Chem. 1988, 52, 873-878.
81. Angsuthanasombat, C.; Uawithya, P.; Leetachewa, S.; Pornwiroon, W.; Ounjai, P.; Kerdcharoen, T.; Katzenmeier, G. Panyim, S. Bacillus thuringiensis Cry4A and Cry4B mosquito-larvicidal proteins: Homology-based 3D model and implications for toxin activity. J. Biochem. Molec. Biol. 2004, 37, 304-313.
82. Revina, L.P.; Kostina, L.I.; Ganushkina, L.A.; Mikhailova, A.L.; Zalunin, I.A.; Chestukhina, G.G. Reconstruction of Bacillus thuringiensis ssp. israelensis Cry11A endotoxin from fragments corresponding to its N-and C moieties restores its original biological activity. Biochemistry 2004, 69, 181-187.
83. Thiery, I.; Delecluse, A.; Tamayo, M.C.; Orduz, S. Identification of a gene for Cyt1A-like hemolysin from Bacillus thuringiensis subsp. medellin and expression in a crystal-negative B. thuringiensis strain. Appl. Environ. Microbiol. 1997, 63, 468-473.
84. Bourgouin, C.; Klier, A.; Rapoport, G. Characterization of the genes encoding the haemolytic toxin and mosquitocidal δ-endotoxin of Bacillus thuringiensis var. israelensis. Mol. Gen. Genet. 1986, 205, 390-397.
85. Thomas, W.E.; Ellar, D.J. Bacillus thuringiensis var. israelensis crystal δ-endotoxin: Effects on insect and mammalian cells in vitro and in vivo. J. Cell Sci. 1983, 60, 181-197.
86. Cheong, H.; Gill, S.S. Cloning and characterization of a cytolytic and mosquitocidal δ-endotoxin from Bacillus thuringiensis subsp. jegathesan. Appl. Environ. Microbiol. 1997, 63, 3254-3260.
87. Crickmore, N.; Zeigler, D.R.; Feitelson, J.; Schnepf, E.; Van Rie, J.; Lereclus, D.; Baum, J.; Dean, D.H. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 1998, 62, 807-813.
88. Georghiou, G.P.; Wirth, M.C. Influence of exposure to single versus multiple toxins of Bacillus thuringiensis subsp. israelensis on development of resistance in the mosquito Culex quinquefasciatus (Diptera: Culicidae). Appl. Environ. Microbiol. 1997, 63, 1095-1101.
89. Wirth, M.C.; Georghiou, G.P. Cross-resistance among CryIV toxins of Bacillus thuringiensis subsp. israelensis in Culex quinquefasciatus (Diptera: Culicidae). J. Econ. Entomol. 1997, 90, 1471-1477.
90. Wirth, M.C.; Georghiou, G.P.; Federici, B.A. CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus. Proc. Natl. Acad. Sci. USA 1997, 94, 10536-10540.
91. Garguno, F.; Thorne, L.; Walfield, A.M.; Pollock, T.J. Structural relatedness between mosquitocidal endotoxins of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 1988, 54, 277-279.
92. Hernández-Soto, A.; Del Rincón-Castro, M.C.; Espinoza, A.M.; Ibarra, J.E. Parasporal body formation via overexpression of the Cry10Aa toxin of Bacillus thuringiensis subsp. israelensis, and Cry10Aa-Cyt1Aa synergism. Appl. Environ. Microbiol. 2009, 75, 4661-4667.
93. Delecluse, A.; Bourgouin, C.; Klier, A.; Rapoport, G. Specificity of action on mosquito larvae of Bacillus thuringiensis var. israelensis toxins encoded by two different genes. Mol. Gen. Genet. 1988, 214, 42-47.
94. Juárez-Pérez, V.; Guerchicoff, A.; Rubinstein, C.; Delécluse, A. Characterization of Cyt2Bc toxin from Bacillus thuringiensis subsp. medellin. Appl. Environ. Microbiol. 2002, 68, 1228-1231.
95. Nisnevitch, M.; Cohen, S.; Ben-Dov, E.; Zaritsky, A.; Sofer, Y.; Cahan, R. Cyt2Ba of Bacillus thuringiensis israelensis: Activation by putative endogenous protease Biochem. Biophys. Res. Commun. 2006, 344, 99-105.
96. Wirth, M.C.; Delécluse, A.; Walton, W.E. Cyt1Ab1 and Cyt2Ba1 from Bacillus thuringiensis subsp. medellin and B. thuringiensis subsp. israelensis synergize Bacillus sphaericus against Aedes aegypti and resistant Culex quinquefasciatus (Diptera: Culicidae). Appl. Environ. Microbiol. 2001, 67, 3280-3284.
97. Purcell, M.; Ellar, D.J. The identification and characterization of novel proteinacious components of the Bacillus thuringiensis subsp. israelensis parasporal inclusion, In Proceedings of the 30th Annual Meeting of the Society for Invertebrate Pathology, Banff, Canada, 24-29 August 1997; p. 53.
98. Manasherob, R.; Itsko, M.; Sela-Baranes, N.; Ben-Dov, E.; Berry, C.; Cohen, S.; Zaritsky, A. Cyt1Ca from Bacillus thuringiensis subsp. israelensis: production in Escherichia coli and comparison of its biological activities with those of other Cyt-like proteins. Microbiology 2006, 152, 2651-2659.
99. Itsko, M.; Zaritsky, A. Exposing cryptic antibacterial activity in Cyt1Ca from Bacillus thuringiensis israelensis by genetic manipulations. FEBS Lett. 2007, 581, 1775-1782.
100. Stein, C.; Jones, G.W.; Chalmers, T.; Berry, C. Transcriptional analysis of the toxin-coding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 2006, 72, 1771-1776.
101. Cohen, S.; Albeck, S.; Ben-Dov, E.; Cahan, R.; Firer, M.; Zaritsky, A.; Dym, O. Cyt1Aa toxin: Crystal structure reveals implications for its membrane-perforating function. J. Mol. Biol. 2011, 413, 804-814.
102. Angsuthanasombat, C. Structural basis of pore formation by mosquito-larvicidal proteins from Bacillus thuringiensis. Open Toxinol. J. 2010, 3, 119-125.
103. Boonserm, P.; Angsuthanasombat, C.; Lescar, J. Crystallization and preliminary crystallographic study of the functional form of the Bacillus thuringiensis mosquito-larvicidal Cry4Aa mutant toxin. Acta Crystallogr. Sect. D 2004. 60, 1315-1318.
104. Boonserm, P.; Davis, P.; Ellar, D.J.; Li, J. Crystal structure of the mosquito-larvicidal toxin Cry4Ba and its biological implications. J. Mol. Biol. 2005, 348, 363-382.
105. Boonserm, P.; Mo, M.; Angsuthanasombat, C.; Lescar, J. Structure of the functional form of the mosquito larvicidal Cry4Aa toxin from Bacillus thuringiensis at a 2.8-angstrom resolution. J. Bacteriol. 2006, 188, 3391-3401.
106. Grochulski, P.; Masson, L.; Borisova, S.; Puztai-Carey, M.; Schwartz, J.-L.; Brousseau, R.; Cygler, M. Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. J. Mol. Biol. 1995, 254, 447-464.
107. Li, J.; Carroll, J.; Ellar, D.J. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5 Å resolution. Nature 1991, 353, 815-821.
108. Morse, R.J.; Yamamoto, T.; Stroud, R.M. Structure of Cry2Aa suggests an unexpected receptor binding epitope. Structure 2001, 9, 409-417.
109. Komano, T.; Yamigawa, M.; Nishimoto, T.; Yoshisue, H.; Tanabe, K.; Sen, K.; Sakai, H. Activation process of the insecticidal proteins CryIVA and CryIVB produced by Bacillus thuringiensis subsp. israelensis. Isr. J. Entomol. 1998, 32, 185-198.
110. Yamagiwa, M.; Esaki, M.; Otake, K.; Inagaki, M.; Komano, T.; Amachi, T.; Sakai, H. Activation process of dipteran-specific insecticidal protein produced by Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 1999, 65, 3464-3469.
111. Dai, S.-M.; Gill. S.S. In vitro and in vivo proteolysis of the Bacillus thuringiensis subsp. israelensis CryIVD protein by Culex quinquefasciatus larval midgut proteases. Insect Biochem. Molec. Biol. 1993, 23, 273-283.
112. Yamagiwa, M.; Ogawa, R.; Yasuda, K.; Natsuyama, H.; Sen, K.; Sakai, H. Active form of dipteran-specific insecticidal protein Cry11A produced by Bacillus thuringiensis subsp. israelensis. Biosci. Biotech. Biochem. 2002, 66, 516-522.
113. Yamagiwa, M.; Sakagawa, K.; Sakai, H. Functional analysis of two processed fragments of Bacillus thuringiensis Cry11A toxin. Biosci. Biotech. Biochem. 2004, 68, 523-528.
114. Bravo, A.; Gill, S.S. Soberon, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon 2007, 49, 423-435.
115. Fernandez, L.E.; Perez, C.; Segovia, L.; Rodriguez, M.H.; Gill, S.S.; Bravo, A.; Soberon, M. Cry11Aa toxin from Bacillus thuringiensis binds its receptor in Aedes aegypti mosquito larvae through loop α-8 of domain II. FEBS Lett. 2005, 579, 3508-3514.
116. Chayaratanasin, P.; Moonsom, S.; Sakdee, S.; Chaisri, U.; Katzenmeier, G.; Angsuthanasombat, C. High level of soluble expression in Escherichia coli and characterisation of the cloned Bacillus thuringiensis Cry4Ba Domain III fragment. J. Biochem. Mol. Biol. 2007, 40, 58-64.
117. Knowles, B.H. Mechanism of action of Bacillus thuringiensis insecticidal δ-endotoxins. Adv. Insect Physiol. 1994, 24, 275-308.
118. Aronson, A.I.; Shai, Y. Why Bacillus thuringiensis insecticidal toxins are so effective: Unique features of their mode of action. FEMS Microbiol. Lett. 2001, 195, 1-8.
119. Knowles, B.H.; Ellar, D.J. Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis δ-endotoxins with different insect specificities. Biochim. Biophys. Acta 1987, 924, 509-518.
120. Ravoahangimalala, O.; Charles, J.-F. In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae (Diptera: Culicidae). FEBS Lett. 1995, 362, 111-115.
121. Howlader, M.T.H.; Kagawa, Y.; Sakai, H.; Hayakawa, T. Biological properties of loop-replaced mutants of Bacillus thuringiensis mosquitocidal Cry4Aa. J. Biosci. Bioeng. 2009, 108, 179-183.
122. Buzdin, A.A.; Revina, L.P.; Kostina, L.I.; Zalunin, I.A.; Chestukhina, G.G. Interaction of 65-and 62-kD proteins from the apical membranes of the Aedes aegypti larvae midgut epithelium with Cry4B and Cry11A endotoxins of Bacillus thuringiensis. Biochemistry 2002, 67, 540-546.
123. Saengwiman, S.; Aroonkesorn, A.; Dedvisitsakul, P.; Sakdee, S.; Leetachewa, S.; Angsuthanasombat, C.; Pootanakit, K. In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae. Biochem. Biophys. Res. Commun. 2011, 407, 708-713.
124. Thammasittirong, A.; Dechklar, M.; Leetachewa, S.; Pootanakit, K.; Angsuthanasombat, C. Aedes aegypti membrane-bound alkaline phosphatase expressed in Escherichia coli retains high-affinity binding for Bacillus thuringiensis Cry4Ba toxin. Appl. Environ. Microbiol. 2011, 77, 6836-6840.
125. Fernandez-Luna, M.T.; Lanz-Mendoza, H.; Gill, S.S.; Bravo, A.; Soberon, M.; Miranda-Rios, J. An α-amylase is a novel receptor for Bacillus thuringiensis ssp. israelensis Cry4Ba and Cry11Aa toxins in the malaria vector mosquito Anopheles albimanus (Diptera: Culicidae). Environ. Microbiol. 2010, 12, 746-757.
126. Howlader, M.T.H.; Kagawa, Y.; Miyakawa, A.; Yamamoto, A.; Taniguchi, T.; Hayakawa, T.; Sakai, H. Alanine scanning analyses of the three major loops in domain II of Bacillus thuringiensis mosquitocidal toxin Cry4Aa. Appl. Environ. Microbiol. 2010, 76, 860-865.
127. Likitvivatanavong, S.; Katzenmeier, G.; Angsuthanasombat, C. Asn183 in α5 is essential for oligomerisation and toxicity of the Bacillus thuringiensis Cry4Ba toxin. Arch. Biochem. Biophys. 2006, 445, 46-55.
128. Taveecharoenkool, T.; Angsuthanasombat, C.; Kantchanawarin, C. Combined molecular dynamics and continuum solvent studies of the pre-pore Cry4Aa trimer suggest its stability in solution and how it may form a pore. PMC Biophys. 2010, 3, 1-16.
129. Rodríguez-Almazán, C.; Reyes, E.Z.; Zúñiga-Navarrete, F.; Muñoz-Garay, C.; Gómez, I.; Evans, A.M.; Likitvivatanavong, S.; Bravo, A.; Gill, S.S.; Soberón, M. Cadherin binding is not a limiting step for Bacillus thuringiensis subsp. israelensis Cry4Ba toxicity to Aedes aegypti larvae. Biochem. J. 2012, 443, 711-717.
130. Chen, J.; Aimanova, K.G.; Fernandez, L.E.; Bravo, A.; Soberon, M.; Gill, S.S. Aedes aegypti cadherin serves as a putative receptor of the Cry11Aa toxin from Bacillus thuringiensis subsp. israelensis. Biochem. J. 2009, 424, 191-200.
131. Chen, J.; Aimanova, K.G.; Pan, S.; Gill, S.S. Identification and characterization of Aedes aegypti aminopeptidase N as a putative receptor of Bacillus thuringiensis Cry11A toxin. Insect Biochem. Mol. Biol. 2009, 39, 688-699.
132. Cohen, S.; Dym, O.; Albeck, S.; Ben-Dov, E.; Cahan, R.; Firer, M.; Zaritsky, A. High-resolution crystal structure of activated Cyt2Ba monomer from Bacillus thuringiensis subsp. israelensis. J. Mol. Biol. 2008, 380, 820-827.
133. Li, J.; Koni, P.A.; Ellar, D.J. Structure of the mosquitocidal δ-endotoxin CytB from Bacillus thuringiensis sp. kyushuensis and implications for membrane pore formation. J. Mol. Biol. 1996, 257, 129-152.
134. Lin, S.C.; Lo, Y.C.; Lin, J.Y.; Liaw, Y.C. Crystal structures and electron micrographs of fungal volvatoxin A2. J. Mol. Biol. 2004, 343, 477-491.
135. Gill, S.S.; Singh, G.J.P.; Hornung, J.M. Cell membrane interaction Bacillus thuringiensis subsp. israelensis cytolytic toxins. Infec. Immunol. 1987, 55, 1300-1308.
136. Al-yahyaee, S.A.S.; Ellar, D.J. Maximal toxicity of cloned CytA δ-endotoxin from Bacillus thuringiensis subsp. israelensis requires proteolytic processing from both the N-and C-termini. Microbiology 1995, 141, 3141-3148.
137. Cahan, R.; Friman, H.; Nitzan, Y. Antibacterial activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis. Microbiology 2008, 154, 3529-3536.
138. Butko, P.; Huang, F.; Pusztai-Carey, M.; Surewicz, W.K. Membrane permeabilization induced by cytolytic δ-endotoxin CytA from Bacillus thuringiensis var. israelensis. Biochemistry 1996, 35, 11355-11360.
139. Butko, P.; Huang, F.; Pusztai-Carey, M.; Surewicz, W.K. Interaction of the δ-endotoxin CytA from Bacillus thuringiensis var. israelensis with lipid membranes. Biochemistry 1997, 36, 12862-12868.
140. Koni, P.A.; Ellar, D.J. Cloning and characterization of novel Bacillus thuringiensis cytolitic delta-endotoxin. J. Mol. Biol. 1993, 229, 319-327.
141. Promdonkoy, B.; Ellar, D.J. Membrane pore architecture of a cytolytic toxin from Bacillus thuringiensis. Biochem. J. 2000, 350, 275-282.
142. Promdonkoy, B.; Ellar, D.J. Investigation of the poreforming mechanism of a cytolytic δ-endotoxin from Bacillus thuringiensis. Biochem. J. 2003, 374, 255-259.
143. Manceva, S.D.; Pusztai-Carey, M.; Russo, P.S.; Butko, P. A detergent-like mechanism of action of the cytolytic toxin Cyt1A from Bacillus thuringiensis var. israelensis. Biochemistry 2005, 44, 589-597.
144. Rodriguez-Almazan, C.; Ruiz de Escudero, I.; Canton, P.E.; Munoz-Garay, C.; Perez, C.; Gill, S.S.; Soberón, M.; Bravo, A. The amino-and carboxyl-terminal fragments of the Bacillus thuringensis Cyt1Aa toxin have differential roles in toxin oligomerization and pore formation. Biochemistry 2010, 50, 388-396.
145. Li, J.; Derbyshire, D.J.; Promdonkoy, B.; Ellar, D.J. Structural implications for the transformation of the Bacillus thuringiensis delta-endotoxins from water-soluble to membrane-inserted forms. Biochem. Soc. Trans. 2001, 29, 571-577.
146. Knowles, B.H.; Blatt, M.R.; Tester, M.; Horsnell, J.M.; Carroll, J.; Menestrina, G.; Ellar, D.J. A cytolytic δ-endotoxin from Bacillus thuringiensis var. israelensis forms cation-selective channels in planar lipid bilayers. FEBS Lett. 1989, 244, 259-262.
147. Butko, P. Cytolytic toxin Cyt1A and its mechanism of membrane damage: Data and hypotheses. Appl. Environ. Microbiol. 2003, 69, 2415-2422.
148. Van Frankenhuyzen, K. Cross-order and cross-phylum activity of Bacillus thuringiensis pesticidal proteins. J. Invertebr. Pathol. 2013, 114, 76-85.
149. Delecluse, A.; Poncet, S.; Klier, A.; Rapoport, G. Expression of cryIVA and cryIVB genes, independently or in combination, in a crystal-negative strain of Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 1993, 59, 3922-3927.
150. Khasdan, V.; Ben-Dov, E.; Manasherob, R.; Boussiba, S.; Zaritsky, A. Toxicity and synergism in transgenic Escherichia coli expressing four genes from Bacillus thuringiensis subsp. israelensis. Environ. Microbiol. 2001, 3, 798-806.
151. Khasdan, V.; Ben-Dov, E.; Manasherob, R.; Boussiba, S.; Zaritsky, A. Mosquito larvicidal activity of transgenic Anabaena PCC 7120 expressing toxin genes from Bacillus thuringiensis ssp. israelensis. FEMS Microbiol. Lett. 2003, 227, 189-195.
152. Wirth, M.C.; Zaritsky, A.; Ben-Dov, E.; Manasherob, R.; Khasdan, V.; Boussiba, S.; Walton, W.E. Cross-resistance spectra of Culex quinquefasciatus resistant to mosquitocidal toxins of Bacillus thuringiensis towards recombinant Escherichia coli expressing genes from B. thuringiensis ssp. israelensis. Environ. Microbial. 2007, 9, 1393-1401.
153. Wu, D.; Johnson, J.J.; Federici, B.A. Synergism of mosquitocidal toxicity between CytA and CryIVD proteins using inclusions produced from cloned genes of Bacillus thuringiensis. Mol. Microbiol. 1994, 13, 965-972.
154. Pérez, C.; Fernandez, L.E.; Sun, J.; Folch, J.L.; Gill, S.S.; Soberón, M.; Bravo, A. Bacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor. Proc. Natl. Acad. Sci. USA 2005, 102, 18303-18308.
155. Lailak, C.; Khaokhiew, T.; Promptas, C.; Promdonkoy, B.; Pootanakit, K.; Angsuthanasombat, C. Bacillus thuringiensis Cry4Ba toxin employs two receptor-binding loops for synergistic interactions with Cyt2Aa2. Biochem. Biophys. Res. Commun. 2013, 435, 216-221.
156. Promdonkoy, B.; Promdonkoy, P.; Panyim, S. Co-expression of Bacillus thuringiensis Cry4Ba and Cyt2Aa2 in Escherichia coli revealed high synergism against Aedes aegypti and Culex quinquefasciatus larvae. FEMS Microbiol. Lett. 2005, 252, 121-126.
157. Chang, C.; Yu, Y.-M.; Dai, S.-M.; Law, S.K.; Gill, S.S. High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes. Appl. Environ. Microbiol. 1993, 59, 815-821.
158. Pérez, C.; Muñoz-Garay, C.; Portugal, L.C.; Sánchez, J.; Gill, S.S.; Soberón, M.; Bravo, A. Bacillus thuringiensis ssp. israelensis Cyt1Aa enhances activity of Cry11Aa toxin by facilitating the formation of a pre-pore oligomeric structure. Cell. Microbiol. 2007, 9, 2931-2937.
159. Cantón, P.E.; Reyes, E.Z.; De Escudero, I.R.; Bravo, A.; Soberón, M. Binding of Bacillus thuringiensis subsp. israelensis Cry4Ba to Cyt1Aa has an important role in synergism. Peptides 2011, 32, 595-600.
160. López-Diaz, J.A.; Cantón, P.E.; Gill, S.S.; Soberón, M.; Bravo, A. Oligomerization is a key step in Cyt1Aa membrane insertion and toxicity but not necessary to synergize Cry11Aa toxicity in Aedes aegypti larvae. Environ. Microbiol. 2013, 15, 3030-3039.
161. Wirth, M.C.; Walton, W.E.; Federici, B.A. Inheritance, stability, and dominance of Cry resistance in Culex quinquefasciatus (Diptera: Culicidae) selected with the three Cry toxins of Bacillus thuringiensis subsp. israelensis. J. Med. Entomol. 2012, 49, 886-894.
162. Douek, J.; Einav, M.; Zaritsky, A. Sensitivity to plating of Escherichia coli cells expressing the cytA gene from Bacillus thuringiensis var. israelensis. Mol. Gen. Genet. 1992, 232, 162-165.
163. Manasherob, R.; Zaritsky, A.; Metzler, Y.; Ben-Dov, E.; Itsko, M.; Fishov, I. Compaction of the Escherichia coli nucleoid caused by Cyt1Aa. Microbiology 2003, 149, 3553-3564.
164. Sazhenskiy, V.; Zaritsky, A.; Itsko, M. Expression in Escherichia coli of the native cyt1Aa from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 2010, 76, 3409-3411.
165. Manasherob, R.; Zaritsky, A.; Ben-Dov, E.; Saxena, D.; Barak, Z.; Einav, M. Effect of accessory proteins P19 and P20 on cytolytic activity of Cyt1Aa from Bacillus thuringiensis subsp. israelensis in Escherichia coli. Curr. Microbiol. 2001, 43, 355-364.
166. Revina, L.P.; Kostina, L.I.; Dronina, M.A.; Zalunin, I.A.; Chestukhina, G.G.; Yudina, T.G.; Konukhova, A.V.; Izumrudova, A.V. Novel antibacterial proteins from entomocidal crystals of Bacillus thuringiensis ssp. israelensis. Can. J. Microbiol. 2005, 51, 141-148.
167. Yudina, T.G.; Konukhova, A.V.; Revina, L.P.; Kostina, L.I.; Zalunin, I.A.; Chestukhina, G.G. Antibacterial activity of Cry-and Cyt-proteins from Bacillus thuringiensis ssp. israelensis. Can. J. Microbiol. 2003, 49, 37-44.
168. Yudina, T.G.; Brioukhanov, A.L.; Zalunin, I.A.; Revina, L.P.; Shestakov, A.I.; Voyushina, N.E.; Chestukhina, G.G.; Netrusov, A.I. Antimicrobial activity of different proteins and their fragments from Bacillus thuringiensis parasporal crystals against clostridia and archaea. Anaerobe 2007, 13, 6-13.
169. Cohen, S.; Cahan, R.; Ben-Dov, E.; Nisnevitch, M.; Zaritsky, A.; Firer, M.A. Specific targeting to murine myeloma cells of Cyt1Aa toxin from Bacillus thuringiensis subspecies israelensis. J. Biol. Chem. 2007, 282, 28301-28308.
170. Corrêa, R.F.T.; Ardisson-Araújo, D.M.P.; Monnerat, R.G.; Ribeiro, B.M. Cytotoxicity analysis of three Bacillus thuringiensis subsp. israelensis δ-endotoxins towards insect and mammalian cells. PLoS ONE 2012, 7, e46121.
171. Al-yahyaee, S.A.S.; Ellar, D.J. Cell targeting of a pore-forming toxin, CytA δ-endotoxin from Bacillus thuringiensis subspecies israelensis, by conjugating CytA with anti-Thy 1 monoclonal antibodies and insulin. Bioconjug. Chem. 1996, 7, 451-460.
172. Park, H.-W.; Bideshi, D.K.; Wirth, M.C.; Johnson, J.J.; Walton, W.E.; Federici, B.A. Recombinant larvicidal bacteria with markedly improved efficacy against Culex vectors of West Nile virus. Am. J. Trop. Med. Hyg. 2005, 72, 732-738.
173. Park, H.-W.; Bideshi, D.K.; Federici, B.A. Recombinant strain of Bacillus thuringiensis producing Cyt1A, Cry11B, and the Bacillus sphaericus binary toxin. Appl. Environ. Microbiol. 2003, 69, 1331-1334.
174. Boussiba, S.; Wu, X.-Q.; Ben-Dov, E.; Zarka, A.; Zaritsky, A. Nitrogen-fixing cyanobacteria as gene delivery system for expressing mosquitocidal toxins of Bacillus thuringiensis subsp. israelensis. J. Appl. Phycol. 2000, 12, 461-467.
175. Lluisma, A.O.; Karmacharya, N.; Zarka, A.; Ben-Dov, E.; Zaritsky, A.; Boussiba, S. Suitability of Anabaena PCC 7120 expressing mosquitocidal toxin genes from Bacillus thuringiensis subsp. israelensis for biotechnological application. Appl. Microbiol. Biotechnol. 2001, 57, 161-166.
176. Wu, X.; Vennison, S.J.; Liu, H.; Ben-Dov, E.; Zaritsky, A.; Boussiba, S. Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis. Appl. Environ. Microbiol. 1997, 63, 4971-4974.
177. Manasherob, R.; Ben-Dov, E.; Wu, X.; Boussiba, S.; Zaritsky, A. Protection from UV-B damage of mosquito larvicidal toxins from Bacillus thuringiensis subsp. israelensis expressed in Anabaena PCC 7120. Curr. Microbiol. 2002, 45, 217-220.
178. Ketseoglou, I.; Bouwer, G. The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes. Parasites Vectors 2012, 5, doi:10.1186/1756-3305-5-220.
179. Ketseoglou, I.; Bouwer, G. Optimization of photobioreactor growth conditions for a cyanobacterium expressing mosquitocidal Bacillus thuringiensis Cry proteins. J. Biotechnol. 2013, 167, 64-71.
180. Sun, Y.; Zhao, Q.; Zheng, D.; Ding, X.; Wang, J.; Hu, Q.; Yuan, Z.; Park, H.-W.; Xia, L. Construction and characterization of the interdomain chimeras using Cry11Aa and Cry11Ba from Bacillus thuringiensis and identification of a possible novel toxic chimera. Biotechnol. Lett. 2014, 36, 105-111.
181. Sirichotpakorn, N.; Rongnoparut, P.; Choosang, K.; Panbangred, W. Coexpression of chitinase and the cry11Aa1 toxin genes in Bacillus thuringiensis serovar israelensis. J. Invertebr. Pathol. 2001, 78, 160-169.
182. Borovsky, D.; Khasdan, V.; Nauwelaers, S.; Theunis, C.; Bertier, L.; Ben-Dov, E.; Zaritsky, A. Synergy between Aedes aegypti trypsin modulating oostatic factor and δ-endotoxins. Open Toxinol. J. 2010, 3, 116-125.
183. Borovsky, D.; Nauwelaers, S.; Van Mileghem, A.; Meyvis, Y.; Laeremans, A.; Theunis, C.; Bertier, L.; Boons, E. Control of mosquito larvae with TMOF and 60 kDa Cry4Aa expressed in Pichia pastoris. Pestycydy 2011, 1, 5-15.