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Volumn 9, Issue 3, 2014, Pages

Reconstruction of mouse testicular cellular microenvironments in long-term seminiferous tubule culture

Author keywords

[No Author keywords available]

Indexed keywords

CD9 ANTIGEN; CELL PROTEIN; FOLLITROPIN; G PROTEIN COUPLED RECEPTOR; G PROTEIN COUPLED RECEPTOR 125; GLIAL CELL LINE DERIVED NEUROTROPHIC FACTOR; LIN28 PROTEIN; MESSENGER RNA; PROMYELOCYTIC LEUKAEMIA ZINC FINGER PROTEIN; STEM CELL FACTOR RECEPTOR; TRANSCRIPTION FACTOR NANOG; UNCLASSIFIED DRUG; WT1 PROTEIN; BIOLOGICAL MARKER; CULTURE MEDIUM; TESTOSTERONE;

EID: 84897503893     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0090088     Document Type: Article
Times cited : (25)

References (61)
  • 4
    • 33646336640 scopus 로고    scopus 로고
    • Pluripotency of spermatogonial stem cells from adult mouse testis
    • Guan K, Nayernia K, Maier L, Wagner S, Dressel R, et al. (2006) Pluripotency of spermatogonial stem cells from adult mouse testis. Nature 440: 1199-1203.
    • (2006) Nature , vol.440 , pp. 1199-1203
    • Guan, K.1    Nayernia, K.2    Maier, L.3    Wagner, S.4    Dressel, R.5
  • 5
    • 59849129677 scopus 로고    scopus 로고
    • Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells
    • Kossack N, Meneses J, Shefi S, Nguyen HN, Chavez S, et al. (2009) Isolation and characterization of pluripotent human spermatogonial stem cell-derived cells. Stem Cells 27: 138-149.
    • (2009) Stem Cells , vol.27 , pp. 138-149
    • Kossack, N.1    Meneses, J.2    Shefi, S.3    Nguyen, H.N.4    Chavez, S.5
  • 8
    • 0347382618 scopus 로고    scopus 로고
    • Non-random distribution of spermatogonia in rats: Evidence of niches in the seminiferous tubules
    • Chiarini-Garcia H, Raymer AM, Russell LD (2003) Non-random distribution of spermatogonia in rats: evidence of niches in the seminiferous tubules. Reproduction 126: 669-680. (Pubitemid 37540628)
    • (2003) Reproduction , vol.126 , Issue.5 , pp. 669-680
    • Chiarini-Garcia, H.1    Raymer, A.M.2    Russell, L.D.3
  • 9
    • 0034811373 scopus 로고    scopus 로고
    • High-resolution light microscopic characterization of mouse spermatogonia
    • Chiarini-Garcia H, Russell LD (2001) High-resolution light microscopic characterization of mouse spermatogonia Biol Reprod 65: 1170-1178. (Pubitemid 32927584)
    • (2001) Biology of Reproduction , vol.65 , Issue.4 , pp. 1170-1178
    • Chiarini-Garcia, H.1    Russell, L.D.2
  • 10
    • 34648812826 scopus 로고    scopus 로고
    • A vasculature-associated niche for undifferentiated spermatogonia in the mouse testis
    • DOI 10.1126/science.1144885
    • Yoshida S, Sukeno M, Nabeshima Y (2007) A vasculature-associated niche for undifferentiated spermatogonia in the mouse testis. Science 317: 1722-1726. (Pubitemid 47461832)
    • (2007) Science , vol.317 , Issue.5845 , pp. 1722-1726
    • Yoshida, S.1    Sukeno, M.2    Nabeshima, Y.-I.3
  • 11
    • 84860508756 scopus 로고    scopus 로고
    • Identification and regulation of a stage-specific stem cell niche enriched by nanog positive spermatogonial stem cells in the mouse testis
    • Ventelä S, Mäkelä JA, Kulmala J, Westermarck J, Toppari J (2012) Identification and regulation of a stage-specific stem cell niche enriched by nanog positive spermatogonial stem cells in the mouse testis. Stem Cells 30: 1008-1020
    • (2012) Stem Cells , vol.30 , pp. 1008-1020
    • Ventelä, S.1    Mäkelä, J.A.2    Kulmala, J.3    Westermarck, J.4    Toppari, J.5
  • 12
    • 0022154759 scopus 로고
    • Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation
    • Toppari J, Eerola E, Parvinen M (1985) Flow cytometric DNA analysis of defined stages of rat seminiferous epithelial cycle during in vitro differentiation. J Androl 6: 325-333.
    • (1985) J Androl , vol.6 , pp. 325-333
    • Toppari, J.1    Eerola, E.2    Parvinen, M.3
  • 13
    • 0022158391 scopus 로고
    • In vitro differentiation of rat seminiferous tubular segments from defined stages of the epithelial cycle morphologic and immunolocalization analysis
    • Toppari J, Parvinen M (1985) In vitro differentiation of rat seminiferous tubular segments from defined stages of the epithelial cycle morphologic and immunolocalization analysis. J Androl 6: 334-343.
    • (1985) J Androl , vol.6 , pp. 334-343
    • Toppari, J.1    Parvinen, M.2
  • 14
    • 0022588214 scopus 로고
    • Regulation of stages VI and VIII of the rat seminiferous epithelial cycle in vitro
    • Toppari J, Vihko KK, Räsänen KG, Eerola E, Parvinen M (1985) Regulation of stages VI and VIII of the rat seminiferous epithelial cycle in vitro. J Endocrinol 108: 417-422. (Pubitemid 16160810)
    • (1986) Journal of Endocrinology , vol.108 , Issue.3 , pp. 417-422
    • Toppari, J.1    Vihko, K.K.2    Rasanen, K.G.E.3
  • 16
    • 79953067218 scopus 로고    scopus 로고
    • In vitro production of functional sperm in cultured neonatal mouse testes
    • Sato T, Katagiri K, Gohbara A, Inoue K, Ogonuki N, et al. (2011) In vitro production of functional sperm in cultured neonatal mouse testes. Nature 471: 504-507.
    • (2011) Nature , vol.471 , pp. 504-507
    • Sato, T.1    Katagiri, K.2    Gohbara, A.3    Inoue, K.4    Ogonuki, N.5
  • 17
    • 84887003444 scopus 로고    scopus 로고
    • In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method
    • Sato T, Katagiri K, Kubota Y, Ogawa T (2013) In vitro sperm production from mouse spermatogonial stem cell lines using an organ culture method. Nat Protoc 8: 2098-2104.
    • (2013) Nat Protoc , vol.8 , pp. 2098-2104
    • Sato, T.1    Katagiri, K.2    Kubota, Y.3    Ogawa, T.4
  • 18
    • 84889007624 scopus 로고    scopus 로고
    • In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation
    • Yokonishi T, Sato T, Katagiri K, Komeya M, Kubota Y, et al. (2013) In Vitro Reconstruction of Mouse Seminiferous Tubules Supporting Germ Cell Differentiation. Biol Reprod 89: 1-6.
    • (2013) Biol Reprod , vol.89 , pp. 1-6
    • Yokonishi, T.1    Sato, T.2    Katagiri, K.3    Komeya, M.4    Kubota, Y.5
  • 21
    • 68149156468 scopus 로고    scopus 로고
    • The pluripotency factor LIN28 marks undifferentiated spermatogonia in mouse
    • Zheng K, Wu X, Kaestner KH, Wang PJ (2009) The pluripotency factor LIN28 marks undifferentiated spermatogonia in mouse. BMC Dev Biol 9: 38.
    • (2009) BMC Dev Biol , vol.9 , pp. 38
    • Zheng, K.1    Wu, X.2    Kaestner, K.H.3    Wang, P.J.4
  • 22
    • 0027054125 scopus 로고
    • The expression of the wilms-tumor gene, wt1, in the developing mammalian embryo
    • Armstrong J, Pritchard-Jones K, Bickmore W, Hastie N, Bard J (1993) The expression of the wilms-tumor gene, wt1, in the developing mammalian embryo. Mech Dev 40: 85-97.
    • (1993) Mech Dev , vol.40 , pp. 85-97
    • Armstrong, J.1    Pritchard-Jones, K.2    Bickmore, W.3    Hastie, N.4    Bard, J.5
  • 23
    • 0025236549 scopus 로고
    • Characterization of rat testicular peritubular myoid cells in culture: alpha-smooth muscle isoactin is a specific differentiation marker
    • Tung P,Fritz I (1990) Characterization of rat testicular peritubular myoid cells in culture - alpha-smooth muscle isoactin is a specific differentiation marker. Biol Reprod 42: 351-365. (Pubitemid 20107260)
    • (1990) Biology of Reproduction , vol.42 , Issue.2 , pp. 351-365
    • Tung, P.S.1    Fritz, I.B.2
  • 24
    • 0342980374 scopus 로고    scopus 로고
    • Mage-b4, a novel melanoma antigen (MAGE) gene specifically expressed during germ cell differentiation
    • Österlund C, Töhönen V, Forslund KO, Nordqvist K (2000) Mage-b4, a novel melanoma antigen (MAGE) gene specifically expressed during germ cell differentiation. Cancer Res 15: 1054-1061. (Pubitemid 30129545)
    • (2000) Cancer Research , vol.60 , Issue.4 , pp. 1054-1061
    • Osterlund, C.1    Tohonen, V.2    Forslund, K.O.3    Nordqvist, K.4
  • 25
    • 0347355092 scopus 로고    scopus 로고
    • CD9 Is a Surface Marker on Mouse and Rat Male Germline Stem Cell
    • DOI 10.1095/biolreprod.103.020867
    • Kanatsu-Shinohara M, Toyokuni S, Shinohara T (2004) CD9 is a surface marker on mouse and rat male germline stem cells. Biol Reprod 70: 70-75. (Pubitemid 38063552)
    • (2004) Biology of Reproduction , vol.70 , Issue.1 , pp. 70-75
    • Kanatsu-Shinohara, M.1    Toyokuni, S.2    Shinohara, T.3
  • 26
    • 0026067317 scopus 로고
    • Expression of the c-kit proto-oncogene in the murine male germ cells
    • Sorrentino V, Giorgi M, Geremia R, Besmer P, Rossi P (1991) Expression of the C-Kit Protooncogene in the Murine Male Germ-Cells. Oncogene 6: 149-151. (Pubitemid 21923995)
    • (1991) Oncogene , vol.6 , Issue.1 , pp. 149-151
    • Sorrentino, V.1    Giorgi, M.2    Geremia, R.3    Besmer, P.4    Rossi, P.5
  • 28
    • 34548482497 scopus 로고    scopus 로고
    • Glial cell line-derived neurotrophic factor regulation of genes essential for self-renewal of mouse spermatogonial stem cells is dependent on Src family kinase signaling
    • DOI 10.1074/jbc.M703474200
    • Oatley JM, Avarbock MR, Brinster RL (2007) Glial cell line-derived neurotrophic factor regulation of genes essential for self-renewal of mouse spermatogonial stem cells is dependent on src family kinase signaling. J Biol Chem 282: 25842-25851. (Pubitemid 47372831)
    • (2007) Journal of Biological Chemistry , vol.282 , Issue.35 , pp. 25842-25851
    • Oatley, J.M.1    Avarbock, M.R.2    Brinster, R.L.3
  • 30
    • 0036668635 scopus 로고    scopus 로고
    • Murine spermatogonial stem cells: Targeted transgene expression and purification in an active state
    • DOI 10.1093/embo-reports/kvf149
    • Giuili G, Tomljenovic A, Labrecque N, Oulad-Abdelghani M, Rassoulzadegan M, et al. (2002) Murine spermatogonial stem cells: targeted transgene expression and purification in an active state. EMBO Rep 3: 753-759. (Pubitemid 34966014)
    • (2002) EMBO Reports , vol.3 , Issue.8 , pp. 753-759
    • Giuili, G.1    Tomljenovic, A.2    Labrecque, N.3    Oulad-Abdelghani, M.4    Rassoulzadegan, M.5    Cuzin, F.6
  • 31
    • 46449101976 scopus 로고    scopus 로고
    • Expression of stimulated by retinoic acid gene 8 (Stra8) in Spermatogenic Cells Induced by retinoic acid: An in vivo study in vitamin A-sufficient postnatal murine testes
    • DOI 10.1095/biolreprod.107.066795
    • Zhou Q, Nie R, Li Y, Friel P, Mitchell D, et al. (2008) Expression of stimulated by retinoic acid gene 8 (Stra8) in spermatogenic cells induced by retinoic acid: An in vivo study in vitamin A-sufficient postnatal murine testes. Biol Reprod 79: 35-42. (Pubitemid 351931040)
    • (2008) Biology of Reproduction , vol.79 , Issue.1 , pp. 35-42
    • Zhou, Q.1    Nie, R.2    Li, Y.3    Friel, P.4    Mitchell, D.5    Hess, R.A.6    Small, C.7    Griswold, M.D.8
  • 32
    • 70350448164 scopus 로고    scopus 로고
    • Isolation and short-term culture of mouse spermatocytes for analysis of meiosis
    • La Salle S, Sun F, Handel MA (2009) Isolation and short-term culture of mouse spermatocytes for analysis of meiosis. Methods Mol Biol 558: 279-297.
    • (2009) Methods Mol Biol , vol.558 , pp. 279-297
    • La Salle, S.1    Sun, F.2    Handel, M.A.3
  • 35
    • 0023475289 scopus 로고
    • Effects of busulfan on murine spermatogenesis: Cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations
    • Bucci LR, Meistrich ML (1987) Effects of busulfan on murine spermatogenesis: cytotoxicity, sterility, sperm abnormalities, and dominant lethal mutations. Mutat Res 176: 259-268.
    • (1987) Mutat Res , vol.176 , pp. 259-268
    • Bucci, L.R.1    Meistrich, M.L.2
  • 39
    • 84858858444 scopus 로고    scopus 로고
    • CIP2A promotes proliferation of spermatogonial progenitor cells and spermatogenesis in mice
    • Ventelä S, Côme C, Mäkelä JA, Hobbs RM, Mannermaa L et al. (2012) CIP2A promotes proliferation of spermatogonial progenitor cells and spermatogenesis in mice. PLoS One 7: e33209.
    • (2012) PLoS One , vol.7
    • Ventelä, S.1    Côme, C.2    Mäkelä, J.A.3    Hobbs, R.M.4    Mannermaa, L.5
  • 40
    • 0036325384 scopus 로고    scopus 로고
    • Angiotensin II stimulates contraction and growth of testicular peritubular myoid cells in vitro
    • DOI 10.1210/en.143.8.3096
    • Rossi F, Ferraresi A, Romagni P, Silvestroni L, Santiemma V (2002) Angiotensin II stimulates contraction and growth of testicular peritubular myoid cells in vitro. Endocrinology 143: 3096-3104. (Pubitemid 34809882)
    • (2002) Endocrinology , vol.143 , Issue.8 , pp. 3096-3104
    • Rossi, F.1    Ferraresi, A.2    Romagni, P.3    Silvestroni, L.4    Santiemma, V.5
  • 41
    • 0029091682 scopus 로고
    • Identification and characterization of a fibroblast marker: FSP1
    • Strutz F, Okada H, Lo CW, Danoff T, Carone RL, et al. (1995) Identification and characterization of a fibroblast marker: FSP1. J Cell Biol 130: 393-405.
    • (1995) J Cell Biol , vol.130 , pp. 393-405
    • Strutz, F.1    Okada, H.2    Lo, C.W.3    Danoff, T.4    Carone, R.L.5
  • 42
    • 0024522986 scopus 로고
    • Transforming growth factorb gene expression and action in the seminiferous tubule: Peritubular cell-sertoli cell interactions
    • Skinner MK, Moses HL (1989) Transforming growth factor b gene expression and action in the seminiferous tubule: Peritubular cell-Sertoli interactions. Mol Endocrinol 3: 625-634. (Pubitemid 19108940)
    • (1989) Molecular Endocrinology , vol.3 , Issue.4 , pp. 625-634
    • Skinner, M.K.1    Moses, H.L.2
  • 43
    • 0018941973 scopus 로고
    • Interactions of Sertoli cells with myoid cells in vitro
    • Tung PS, Fritz IB (1980) Interactions of sertoli cells with myoid cells in vitro. Biol Reprod 23:207-217. (Pubitemid 10015339)
    • (1980) Biology of Reproduction , vol.23 , Issue.1 , pp. 207-217
    • Tung, P.S.1    Fritz, I.B.2
  • 44
    • 0032835239 scopus 로고    scopus 로고
    • A role for cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells
    • Rahimi N, Kazlauskas A (1999) A role for Cadherin-5 in regulation of vascular endothelial growth factor receptor 2 activity in endothelial cells. Mol Biol Cell 10: 3401-3407. (Pubitemid 29489570)
    • (1999) Molecular Biology of the Cell , vol.10 , Issue.10 , pp. 3401-3407
    • Rahimi, N.1    Kazlauskas, A.2
  • 45
    • 0021929962 scopus 로고
    • Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta
    • DOI 10.1083/jcb.101.2.363
    • Maruyama I, Bell CE, Majerus PW (1985) Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta. J Cell Biol 101: 363-371. (Pubitemid 15239110)
    • (1985) Journal of Cell Biology , vol.101 , Issue.2 , pp. 363-371
    • Maruyama, I.1    Bell, C.E.2    Majerus, P.W.3
  • 46
    • 0032532336 scopus 로고    scopus 로고
    • Tissue distribution and regulation of murine von Willebrand factor gene expression in vivo
    • Yamamoto K, de Waard V, Fearns C, Loskutoff DJ (1998) Tissue distribution and regulation of murine von Willebrand factor gene expression in vivo. Blood 92: 2791-2801. (Pubitemid 28469093)
    • (1998) Blood , vol.92 , Issue.8 , pp. 2791-2801
    • Yamamoto, K.1    De Waard, V.2    Fearns, C.3    Loskutoff, D.J.4
  • 47
    • 0029952181 scopus 로고    scopus 로고
    • Immunohistochemical detection of immature Sertoli cell markers in testicular tissue of infertile adult men: A preliminary study
    • Steger K, Rey R, Kliesch S, Louis F, Schleicher G, et al. (1996) Immunohistochemical detection of immature Sertoli cell markers in testicular tissue of infertile adult men: a preliminary study. Int J Androl 19: 122-128. (Pubitemid 26158693)
    • (1996) International Journal of Andrology , vol.19 , Issue.2 , pp. 122-128
    • Steger, K.1    Rey, R.2    Kliesch, S.3    Louis, F.4    Schleicher, G.5    Bergmann, M.6
  • 48
    • 33750367920 scopus 로고    scopus 로고
    • Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours
    • Sonne SB, Herlihy AS, Hoei-Hansen CE, Nielsen JE, Almstrup K, et al. (2006) Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours. Virchows Arch 449: 200-206.
    • (2006) Virchows Arch , vol.449 , pp. 200-206
    • Sonne, S.B.1    Herlihy, A.S.2    Hoei-Hansen, C.E.3    Nielsen, J.E.4    Almstrup, K.5
  • 51
    • 0036126116 scopus 로고    scopus 로고
    • Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway
    • PII S0925477302000047
    • Tadokoro Y, Yomogida K, Ohta H, Tohda A, Nishimune Y (2002) Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway. Mech Dev 113: 29-39. (Pubitemid 34233722)
    • (2002) Mechanisms of Development , vol.113 , Issue.1 , pp. 29-39
    • Tadokoro, Y.1    Yomogida, K.2    Ohta, H.3    Tohda, A.4    Nishimune, Y.5
  • 52
  • 53
    • 0017691821 scopus 로고
    • Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization
    • Bellve A, Cavicchia J, Millette C, Obrien D, Bhatnagar Y, et al. (1977) Spermatogenic cells of prepuberal mouse - isolation and morphological characterization. J Cell Biol 74: 68-85. (Pubitemid 8141486)
    • (1977) Journal of Cell Biology , vol.74 , Issue.1 , pp. 68-85
    • Bellve, A.R.1    Cavicchia, J.C.2    Millette, C.F.3
  • 54
    • 0032130527 scopus 로고    scopus 로고
    • The central role of Sertoli cells in spermatogenesis
    • Griswold MD (1998) The central role of Sertoli cells in spermatogenesis. Semin Cell Dev Biol 9: 411-416.
    • (1998) Semin Cell Dev Biol , vol.9 , pp. 411-416
    • Griswold, M.D.1
  • 55
    • 0019767572 scopus 로고
    • The capacity of testicular cells of the postnatal rat to reorganize into histotypic structures
    • Zenzes MT, Engel W (1981) The capacity of testicular cells of the postnatal rat to reorganize into histotypic structures. Differentiation 20: 157-161. (Pubitemid 12141311)
    • (1981) Differentiation , vol.20 , Issue.2 , pp. 157-161
    • Zenzes, M.T.1    Engel, W.2
  • 56
    • 0025303122 scopus 로고
    • Laminin promotes formation of cord-like structures by Sertoli cells in vitro
    • DOI 10.1016/0012-1606(90)90082-T
    • Hadley MA, Weeks BS, Kleinman HK, Dym M (1990) Laminin promotes formation of cord-like structures by Sertoli cells in vitro. Dev Biol 140: 318-327. (Pubitemid 20222470)
    • (1990) Developmental Biology , vol.140 , Issue.2 , pp. 318-327
    • Hadley, M.A.1    Weeks, B.S.2    Kleinman, H.K.3    Dym, M.4
  • 57
    • 33746416626 scopus 로고    scopus 로고
    • De novo morphogenesis of seminiferous tubules from dissociated immature rat testicular cells in xenografts
    • DOI 10.2164/jandrol.05207
    • Gassei K, Schlatt S, Ehmcke J (2006) De novo morphogenesis of seminiferous tubules from dissociated immature rat testicular cells in xenografts. J Androl 27: 611-618. (Pubitemid 44126275)
    • (2006) Journal of Andrology , vol.27 , Issue.4 , pp. 611-618
    • Gassei, K.1    Schlatt, S.2    Ehmcke, J.3
  • 58
    • 84875550330 scopus 로고    scopus 로고
    • Effects of nanostructures and mouse embryonic stem cells on in vitro morphogenesis of rat testicular cords
    • Pan F, Chi L, Schlatt S (2013) Effects of nanostructures and mouse embryonic stem cells on in vitro morphogenesis of rat testicular cords. PLoS One 8: e60054.
    • (2013) PLoS One , vol.8
    • Pan, F.1    Chi, L.2    Schlatt, S.3
  • 59
    • 0017100451 scopus 로고
    • Cell associations and surface features in cultures of juvenile rat seminiferous tubules
    • Eddy E, Kahri A (1976) Cell associations and surface features in cultures of juvenile rat seminiferous tubules. Anat Rec 185: 333-358.
    • (1976) Anat Rec , vol.185 , pp. 333-358
    • Eddy, E.1    Kahri, A.2
  • 60
    • 0030904130 scopus 로고    scopus 로고
    • 'Green mice' as a source of ubiquitous green cells
    • DOI 10.1016/S0014-5793(97)00313-X, PII S001457939700313X
    • Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y (1997) 'Green mice' as a source of ubiquitous green cells. FEBS Lett 407: 313-319. (Pubitemid 27199450)
    • (1997) FEBS Letters , vol.407 , Issue.3 , pp. 313-319
    • Okabe, M.1    Ikawa, M.2    Kominami, K.3    Nakanishi, T.4    Nishimune, Y.5
  • 61
    • 0035710746 scopus 로고    scopus 로고
    • -DeltaDeltaCT method
    • DOI 10.1006/meth.2001.1262
    • Livak K,Schmittgen T (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2∧(2Delta Delta C(t)) method. Methods 25: 402-408. (Pubitemid 34164012)
    • (2001) Methods , vol.25 , Issue.4 , pp. 402-408
    • Livak, K.J.1    Schmittgen, T.D.2


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