3′-end labeling of RNA with [5′-32P]cytidine 3′,5′-Bis(phosphate) and T4 RNA ligase 1

Cold Spring Harbor Protocols

Volumn 2014, Issue 4, 2014, Pages 444-446

  Nilsen, Timothy W ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CYTIDINE DERIVATIVE; POLYNUCLEOTIDE 5' HYDROXYL KINASE; RNA LIGASE; UNCLASSIFIED DRUG; [5 32P] CYTIDINE 3,5 BIS (PHOSPHATE); BACTERIOPHAGE T4 RNA LIGASE 1; CYTIDINE 3',5' DIPHOSPHATE; CYTIDINE 3',5'-DIPHOSPHATE; CYTIDINE DIPHOSPHATE; PHOSPHORUS; RNA; VIRUS PROTEIN;

ARTICLE; INCUBATION TEMPERATURE; ISOTOPE LABELING; LIGATION; RNA TRANSCRIPTION; BACTERIOPHAGE T4; ENZYMOLOGY; METABOLISM; METHODOLOGY; STAINING;

BACTERIOPHAGE T4; CYTIDINE DIPHOSPHATE; PHOSPHORUS RADIOISOTOPES; RNA; RNA LIGASE (ATP); STAINING AND LABELING; VIRAL PROTEINS;

EID: 84897403561     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot080713     Document Type: Article

References (5)

1. Caprara M. 2013. RNA structure determination using chemical methods. Cold Spring Harb Protoc doi: 10.1101/pdb.prot078485.
2. England T. E, Uhlenbeck OC. 1978. 3′-Terminal labelling of RNA with T4 RNA ligase. Nature 275: 560-561.
3. Nilsen T.W. 2013a. Analysis of pre-mRNA splicing using HeLa cell nuclear extracts. Cold Spring Harb Protoc doi: 10.1101/pdb.prot075184.
4. Nilsen T.W. 2013b. RNA structure determination using nuclease digestion. Cold Spring Harb Protoc doi: 10.1101/pdb.prot072330.
5. Rio D.C. 2014. Electrophoretic mobility shift assays for RNA-protein complexes. Cold Spring Harb Protoc doi: 10.1101/pdb.prot080721.