Analysis of pre-mRNA splicing using HeLa cell nuclear extracts

Cold Spring Harbor Protocols

Volumn 8, Issue 6, 2013, Pages 584-587

  Nilsen, Timothy W ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; PHOSPHORUS; RNA PRECURSOR;

ARTICLE; ELECTROPHORESIS; ELECTROPHORETIC MOBILITY; HELA CELL; PRECIPITATION; RNA SPLICING; SOLUBILIZATION; CELL NUCLEUS; CYTOLOGY; DENATURING GRADIENT GEL ELECTROPHORESIS; HUMAN; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METABOLISM; METHODOLOGY; HELA CELL LINE; PROCEDURES;

CELL EXTRACTS; CELL NUCLEUS; CYTOLOGICAL TECHNIQUES; DENATURING GRADIENT GEL ELECTROPHORESIS; HELA CELLS; HUMANS; ISOTOPE LABELING; PHOSPHORUS RADIOISOTOPES; RNA PRECURSORS; RNA SPLICING;

EID: 84878893128     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot075184     Document Type: Article

References (2)

1. Nilsen TW. 2013. Preparation of nuclear extracts from HeLa cells. Cold Spring Harb Protoc doi: 10.1101/pdb.prot075176.
2. Yu Y-T, Maroney P, Nilsen TW. 1993. Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5′ exon. Cell 75: 1049-1059.