Quantitative absorption cytometry for measuring red blood cell hemoglobin mass and volume

Cytometry Part A

Volumn 85, Issue 4, 2014, Pages 332-338

  Schonbrun, Ethan ^a   Malka, Roy ^b,c   Di Caprio, Giuseppe ^a   Schaak, Diane ^a   Higgins, John M ^b,c  

^a HARVARD UNIVERSITY   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cell volume; Hemoglobin mass; Microfluidics; Quantitative absorption cytometry; Red blood cells

Indexed keywords

HEMOGLOBIN;

CELL SIZE; CYTOLOGY; ERYTHROCYTE; FLOW CYTOMETRY; HUMAN; MEAN CORPUSCULAR VOLUME; MICROFLUIDIC ANALYSIS; PROCEDURES;

CELL SIZE; ERYTHROCYTE INDICES; ERYTHROCYTES; FLOW CYTOMETRY; HEMOGLOBINS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 84897113255     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22450     Document Type: Article

References (26)

1. Wintrobe MM, Greer JP. Clinical Hematology. Philadelphia: Lea and Febiger; 1981.
2. Higgins JM, Mahadevan L. Physiological and pathological population dynamics of circulating human red blood cells. Proc Natl Acad Sci USA 2010;107:20587-20592.
3. Tycko DH, Metz MH, Epstein EA, Grinbaum A. Flow-cytometric light scattering measurement of red blood cell volume and hemoglobin concentration. App Opt 1985;24:1355-1365.
4. Popescu G. Quantitative Phase Imaging of Cells and Tissues. New York: McGraw-Hill Biophotonics; 2011.
5. Rappaz B, Barbul A, Emery Y, Korenstein R, Depeursinge C, Magistretti PF, Marquet P. Comparative study of human erythrocytes by digital holographic microscopy, confocal microscopy, and impedence volume analyzer. Cytometry A 2008;73A:895-903.
6. Kim YR, Ornstein L. Isovolumetric sphering of erythrocytes for more accurate and precise cell volume measurement by flow cytometry. Cytometry 1983;3:419-427.
7. Steinberg MH, Forget BG, Higgs DR, Nagel RL, Franklin Bunn H. Disorders of Hemoglobin: Genetics, Pathophysiology, and Clinical Management. Cambridge, UK: Cambridge University Press; 2000.
8. Park Y, Yamauchi T, Choi W, Dasari R, Feld MS. Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. Opt Lett 2009;34:3668-3670.
9. Mir M, Tangella K, Popescu G. Blood testing at the single cell level using quantitative phase and amplitude microscopy. Opt Exp 2011;2:3259-3266.
10. Gorthi SS, Schonbrun E. Phase imaging flow cytometry using a focus-stack collecting microscope. Opt Lett 2012;37:707-709.
11. Gray ML, Hoffman RA, Hansen WP. A new method for cell volume measurement based on volume exclusion of a fluorescence dye. Cytometry 1983;3:428-434.
12. Model MA, Khitrin AK, Blank JL. Measurement of the absorption of concentrated dyes and their use for quantitative imaging of surface topography. J Micro 2008;231:156-167.
13. Schonbrun E, DiCaprio G, Schaak D. Dye exclusion microfluidic microscopy. Opt Exp 2013;21:8793-8798.
14. Prahl S. Tabulated molar extinction coefficient for hemoglobin in water. Available at: http://omlc.ogi.edu/spectra/hemoglobin/summary.html, 1998.
15. Daimon M, Masumura A. Measurement of the refractive index of distilled water from the near-infrared region to the ultraviolet region. App Opt 2007;46:3811-3820.
16. McFarlane AS. An ultracentrifugal investigation of the serum proteins. Biochem J 1935;29:407-429.
17. Friebel M, Meinke M. Model function to calculate the refractive index of native hemoglobin in the wavelength range of 250-1100 nm dependent on concentration. App Opt 2006;12:2838-2842.
18. Barer R, Tkaczyk S. Refractive index of concentrated protein solutions. Nature 1954;173:821-822.
19. Gregg JL, McGuire KM, Focht DC, Model MA. Measurement of the thickness and volume of adherent cells using transmission-through-dye microscopy. Pflugers Arch 2010;460:1097-1104.
20. Hardie DC, Gregory TR, Hebert PDN. Pixels to pictograms: A beginners' guide to genome quantification by Feulgen image analysis densitometry. Histochem Cytochem 2002;50:735-749.
21. Cheung MC, Evans JG, McKenna B, Ehrlich DJ. Deep ultraviolet mapping of intracellular protein and nucleic acid in femtograms per pixel. Cytometry A 2011;79A:920-932.
22. Evans E, Fung YC. Improved measurement of the erythrocyte geometry. Microvasc Res 1972;4:335-347.
23. Skalak R, Branemark PI. Deformation of red blood cells in capillaries. Science 1969;164:717-719.
24. Basiji DA, Ortyn WE, Liang L, Venkatachalam V, Morrissey P. Cellular image analysis and imaging by flow cytometry. Clin Lab Med 2007;27:653-670.
25. Schonbrun E, Gorthi SS, Schaak D. Microfabricated multiple field of view imaging flow cytometry. Lab Chip 2012;12:268-273.
26. Mohandes N, Gallagher PG. Red cell membrane: Past, present, and future. Blood 2008;15:3939-3948.