15N, 13C and 1H resonance assignments and secondary structure determination of a variable heavy domain of a heavy chain antibody

Biomolecular NMR Assignments

Volumn 8, Issue 1, 2014, Pages 113-116

  Prosser, Christine E ^a,b   Waters, Lorna C ^a   Muskett, Frederick W ^a   Veverka, Vaclav ^a   Addis, Philip W ^a   Griffin, Laura M ^b   Baker, Terry S ^b   Lawson, Alastair D G ^b   Wernery, Ulrich ^c   Kinne, Jorg ^c   Henry, Alistair J ^b   Taylor, Richard J ^b   Carr, Mark D ^a  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UCB 208 Bath Road ^*  (United Kingdom)

^c CENTRAL VETERINARY RESEARCH LABORATORY   (United Arab Emirates)

Author keywords

Heavy chain antibody; NMR resonance assignments; Secondary structure; VHH

Indexed keywords

ANTIBODY; CARBON; HYDROGEN; IMMUNOGLOBULIN HEAVY CHAIN; NITROGEN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CAMEL; CHEMISTRY; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR GENETICS; NUCLEAR MAGNETIC RESONANCE; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; CAMELS; CARBON ISOTOPES; HYDROGEN; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MOLECULAR SEQUENCE DATA; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY;

EID: 84897111575     PISSN: 18742718     EISSN: 1874270X     Source Type: Journal    
DOI: 10.1007/s12104-013-9464-8     Document Type: Article

References (10)

1. 1H NMR methods and chapter 7: Heteronuclear NMR experiments. In: Protein NMR spectroscopy: Principles and practice, 2nd edn. Elsevier Academic Press, Amsterdam, pp 405-678
2. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) J Biomol NMR 6:277-293
3. Hamers-Casterman C, Atarhouch T, Muyldermans S, Robinson G, Hamers C, Bajyana Songa E, Bendahman N, Hamers R (1993) Naturally occurring antibodies devoid of light chains. Nature 363:446-448
4. Muyldermans S, Atarhouch T, Saldanha J, Barbosa JARG, Hamers R (1994) Sequence and structure of VH domain from naturally occurring camel heavy chain immunoglobulins lacking light chains. Protein Eng 7:1129-1135
5. Renisio JG, Perez J, Czisch M, Guenneugues M, Bornet O, Frenken L, Cambillau C, Darbon H (2002) Solution structure and backbone dynamics of an antigen-free heavy chain variable domain (VHH) from llama. Proteins: Structure. Funct Genet 47:546-555
6. Sheriff S, Constantine KL (1996) Redefining the minimal antigen binding fragment. Nat Struct Biol 3:733-736
7. Ward S, Güssow D, Griffiths AD, Jones PT, Winter G (1989) Binding activities of a repertoire of single immunoglobulin variable domains secreted from Escherichia coli. Nature 341:544-546
8. Wilkinson IC, Hall CJ, Veverka V, Shi JY, Muskett F, Stephens PE, Taylor RJ, Henry AJ, Carr MD (2009) High resolution NMR-based model for the structure of a scFv-IL1β complex. J Biol Chem 284:31928-31935
9. Williamson RA, Natalia D, Gee CK, Murphy G, Carr MD, Freedman RB (1996) Chemically and conformationally authentic active domain of human tissue inhibitor of metalloproteinases-2 refolded from bacterial inclusion bodies. Euro J Biochem 241:476-483
10. 13C chemical-shift data. J Biomol NMR 4:171-180