Identification of UshA as a major enzyme for NAD degradation in Escherichia coli

Enzyme and Microbial Technology

Volumn 58-59, Issue , 2014, Pages 75-79

  Wang, Lei ^a,b   Zhou, Yongjin J ^a   Ji, Debin ^a   Lin, Xinping ^a,b   Liu, Yuxue ^a,b   Zhang, Yixin ^a,b   Liu, Wujun ^a   Zhao, Zongbao K ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

5' Nucleotidase; NAD degradation; Nicotinamide adenine dinucleotide; Pyrophosphatase; UshA; Whole cell biocatalysis

Indexed keywords

AMIDES; BIOCHEMISTRY; CATALYST ACTIVITY; ESCHERICHIA COLI;

5'-NUCLEOTIDASE; NICOTINAMIDE ADENINE DINUCLEOTIDES; PYROPHOSPHATASES; USHA; WHOLE-CELL BIOCATALYSIS;

ENZYMES;

ADENOSINE PHOSPHATE; BACTERIAL ENZYME; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE NUCLEOTIDE; NICOTINAMIDE RIBOSIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; UNCLASSIFIED DRUG; USHA ENZYME; 5' NUCLEOTIDASE; ESCHERICHIA COLI PROTEIN; INORGANIC PYROPHOSPHATASE; NAD PYROPHOSPHATASE; PHOSPHODIESTERASE; RECOMBINANT PROTEIN; USHA PROTEIN, E COLI;

ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BIOCATALYSIS; BIOCATALYST; CELL GROWTH; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME METABOLISM; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENE DELETION; HALF LIFE TIME; HYDROLYSIS; NONHUMAN; CELL MEMBRANE PERMEABILITY; CYTOPLASM; ENZYMOLOGY; ESCHERICHIA COLI K 12; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM;

5'-NUCLEOTIDASE; BIOCATALYSIS; CELL MEMBRANE PERMEABILITY; ESCHERICHIA COLI K12; ESCHERICHIA COLI PROTEINS; GENE DELETION; HALF-LIFE; HYDROLYSIS; NAD; NICOTINAMIDE MONONUCLEOTIDE; PERIPLASM; PHOSPHORIC DIESTER HYDROLASES; PYROPHOSPHATASES; RECOMBINANT PROTEINS;

EID: 84897089791     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2014.03.003     Document Type: Article

References (19)

1. Holm A.K., Blank L.M., Oldiges M., Schmid A., Solem C., Jensen P.R., et al. Metabolic and transcriptional response to cofactor perturbations in Escherichia coli. J Biol Chem 2010, 285:17498-17506.
2. San K.Y., Bennett G.N., Berrios-Rivera S.J., Vadali R.V., Yang Y.T., Horton E., et al. Metabolic engineering through cofactor manipulation and its effects on metabolic flux redistribution in Escherichia coli. Metab Eng 2002, 4:182-192.
3. Berrios-Rivera S.J., Bennett G.N., San K.Y. The effect of increasing NADH availability on the redistribution of metabolic fluxes in Escherichia coli chemostat cultures. Metab Eng 2002, 4:230-237.
4. Zhang J., Witholt B., Li Z. Coupling of permeabilized microorganisms for efficient enantioselective reduction of ketone with cofactor recycling. Chem Commun 2006, 39:8-400.
5. Zhang W., O'Connor K., Wang D.I.C., Li Z. Bioreduction with efficient recycling of NADPH by coupled permeabilized microorganisms. Appl Environ Microbiol 2009, 75:687-694.
6. +-auxotrophic mutant. Appl Environ Microbiol 2011, 77:6133-6140.
7. + availability for Escherichia coli whole-cell biocatalysis: a case study for dihydroxyacetone production. Microb Cell Fact 2013, 12:103.
8. Frick D.N., Bessman M.J. Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes. J Biol Chem 1995, 270:1529-1534.
9. Alves-Pereira I., Canales J., Cabezas A., Cordero P.M., Costas M.J., Cameselle J.C. CDP-alcohol hydrolase, a very efficient activity of the 5'-nucleotidase/UDP-sugar hydrolase encoded by the ushA gene of Yersinia intermedia and Escherichia coli. J Bacteriol 2008, 190:6153-6161.
10. Ji D., Wang L., Hou S., Liu W., Wang J., Wang Q., et al. Creation of bioorthogonal redox systems depending on nicotinamide flucytosine dinucleotide. J Am Chem Soc 2011, 133:20857-20862.
11. Baba T., Ara T., Hasegawa M., Takai Y., Okumura Y., Baba M., et al. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2006, 2. 2006.0008.
12. van den Ent F., Lowe J. RF cloning: a restriction-free method for inserting target genes into plasmids. J Biochem Biophys Methods 2006, 67:67-74.
13. Kemmer G., Reilly T.J., Schmidt-Brauns J., Zlotnik G.W., Green B.A., Fiske M.J., et al. NadN and e (P4) are essential for utilization of NAD and nicotinamide mononucleotide but not nicotinamide riboside in Haemophilus influenzae. J Bacteriol 2001, 183:3974-3981.
14. Lee K.S., Song S.B., Kim K.E., Kim Y.H., Kim S.K., Kho B.H., et al. Cloning and characterization of the UDP-sugar hydrolase gene (ushA) of Enterobacter aerogenes IFO 12010. Biochem Biophys Res Commun 2000, 269:526-531.
15. Baykov A.A., Evtushenko O.A., Avaeva S.M. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay. Anal Biochem 1988, 171:266-270.
16. Marchler-Bauer A., Lu S., Anderson J.B., Chitsaz F., Derbyshire M.K., DeWeese-Scott C., et al. CDD: a conserved domain database for the functional annotation of proteins. Nucleic Acids Res 2011, 39:D225-D229.
17. Schomburg I., Chang A., Placzek S., Sohngen C., Rother M., Lang M., et al. BRENDA in 2013: integrated reactions, kinetic data, enzyme function data, improved disease classification: new options and contents in BRENDA. Nucleic Acids Res 2013, 41:D764-D772.
18. Wagner R., Feth F., Wagner K.G. The pyridine-nucleotide cycle in tobacco - enzyme-activities for the recycling of NAD. Planta 1986, 167:226-232.
19. Innes D., Beacham I.R., Burns D.M. The role of the intracellular inhibitor of periplasmic UDP-sugar hydrolase (5'-nucleotidase) in Escherichia coli: cytoplasmic localisation of 5'-nucleotidase is conditionally lethal. J Basic Microbiol 2001, 41:329-337.