The improving effect of reduced glutathione on boar sperm cryotolerance is related with the intrinsic ejaculate freezability

Cryobiology

Volumn 68, Issue 2, 2014, Pages 251-261

  Yeste, Marc ^a   Estrada, Efrén ^a   Pinart, Elisabeth ^b   Bonet, Sergi ^b   Miró, Jordi ^a   Rodríguez Gil, Joan E ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^b UNIVERSITY OF GIRONA   (Spain)

Author keywords

Boar semen; Cryotolerance; Good and poor freezability ejaculates; Reduced glutathione; Sperm cryopreservation

Indexed keywords

GLUTATHIONE; NUCLEOPROTEIN; REACTIVE OXYGEN METABOLITE; CRYOPROTECTIVE AGENT; CYSTEINE; PEROXIDE; SUPEROXIDE;

ACROSOME; ACROSOME MEMBRANE INTEGRITY; ANIMAL CELL; ARTICLE; CELL VIABILITY; CELLULAR PARAMETERS; COLD TOLERANCE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CRYOPRESERVATION; DNA FRAGMENTATION; GOOD FREEZABILITY EJACULATE; MALE; NONHUMAN; POOR FREEZABILITY EJACULATE; PRIORITY JOURNAL; PROTEIN STRUCTURE; SPERM; SPERM CRYOTOLERANCE; SPERM FREEZABILITY; SPERM PRESERVATION; SPERM QUALITY; SPERMATOZOON MOTILITY; SWINE; ANIMAL; CELL SURVIVAL; CHEMISTRY; DRUG EFFECTS; PIG; PROCEDURES; VETERINARY; CELL FUNCTION; CELL INTEGRITY; CLUSTER ANALYSIS; CONCENTRATION RESPONSE; CRYOTOLERANCE; EJACULATE FREEZABILITY; FLOW CYTOMETRY; FREEZING; SUPPLEMENTATION; TEMPERATURE ACCLIMATIZATION; THAWING; THERAPY EFFECT;

ANIMALS; CELL SURVIVAL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; GLUTATHIONE; MALE; SEMEN PRESERVATION; SUS SCROFA;

EID: 84897068589     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2014.02.004     Document Type: Article

References (43)

1. Aitken R.J. Free radicals, lipid peroxidation and sperm function. Reprod. Fertil. Dev. 1995, 7:659-668.
2. Balhorn R. The protamine family of sperm nuclear proteins. Genome Biol. 2007, 8:227-238.
3. Ball B.A., Vo A.T., Baumber J. Generation of reactive oxygen species by equine spermatozoa. Am. J. Vet. Res. 2001, 62:508-515.
4. Baumber J., Ball B.A., Linfor J.J., Meyers S.A. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa. J. Androl. 2003, 24:621-628.
5. Bilodeau J.F., Chatterjee S., Sirard M.A., Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol. Reprod. Dev. 2000, 55:282-288.
6. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
7. Brewer L., Corzett M., Lau E.Y., Balhorn R. Dynamics of protamine-1 binding to single DNA molecules. J. Biol. Chem. 2003, 278:42403-42408.
8. Brocklehurst K., Stuchbury T., Malthouse J.P. Reactivities of neutral and cationic forms of 2,2=-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe. Biochem. J. 1979, 183:233-238.
9. Casas I., Sancho S., Briz M., Pinart E., Bussalleu E., Yeste M., Bonet S. Freezability prediction of boar ejaculates assessed by functional sperm parameters and sperm proteins. Theriogenology 2009, 72:930-948.
10. Casas I., Sancho S., Ballester J., Briz M., Pinart E., Bussalleu E., Yeste M., Fàbrega A., Rodríguez-Gil J.E., Bonet S. The HSP90AA1 sperm content and the prediction of the boar ejaculate freezability. Theriogenology 2010, 74:940-950.
11. Casas I., Flores E. Gene banking: the freezing strategy. Boar Reproduction: Fundamentals and New Biotechnological Trends 2013, 551-588. Springer, Berlin. S. Bonet, I. Casas, W.V. Holt, M. Yeste (Eds.).
12. Chapman J.C., Michael S.D. Proposed mechanism for sperm chromatin condensation/decondensation in the male rat. Reprod. Biol. Endocrinol. 2003, 1:20.
13. De Lamirande E., Gagnon C. Reactive oxygen species and human spermatozoa II: depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl. 1992, 13:379-386.
14. Estrada E., Rodríguez-Gil J.E., Rocha L.G., Balasch S., Bonet S., Yeste M. Supplementing cryopreservation media with reduced glutathione increases fertility and prolificacy of sows inseminated with frozen-thawed boar semen. Andrology 2013, 10.1111/j.2047-2927.2013.00144.x.
15. Flores E., Ramió-Lluch L., Bucci D., Fernández-Novell J.M., Peña A., Rodríguez-Gil J.E. Freezing-thawing induces alterations in histone H1-DNA binding and the breaking of protein-DNA disulphide bonds in boar sperm. Theriogenology 2011, 76:1450-1464.
16. Fraser L., Parda A., Filipowicz K., Strzeżek J. Comparison of post-thaw DNA integrity of boar spermatozoa assessed with the neutral comet assay and Sperm-Sus Halomax test kit. Reprod. Dom. Anim. 2010, 45:155-160.
17. Gadea J., García-Vázquez F.A., Matás C., Gardón J.C., Cánovas S., Gumbao D. Cooling and freezing of boar spermatozoa: supplementation of the freezing media with reduced glutathione preserves sperm function. J. Androl. 2005, 26:396-404.
18. Garner D.L., Johnson L.A. Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol. Reprod. 1995, 53:276-284.
19. Guthrie H.D., Welch G.R. Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated flow cytometry. J. Anim. Sci. 2006, 84:2089-2100.
20. Guthrie H.D., Welch G.R., Long J.A. Mitochondrial function and reactive oxygen species action in relation to boar motility. Theriogenology 2008, 70:1209-1215.
21. Holt W.V. Basic aspects of frozen storage of semen. Anim. Reprod. Sci. 2000, 62:3-22.
22. Holt W.V., Medrano A., Thurston L.M., Watson P.F. The significance of cooling rates and animal variability for boar sperm cryopreservation: insights from the cryomicroscope. Theriogenology 2005, 63:370-382.
23. Jacob C., Giles G.I., Giles N.M., Sies H. Sulfur and selenium: the role of oxidation state in protein structure and function. Angew. Chem. Int. Ed. 2003, 42:4742-4758.
24. Kim S.H., Yu D.H., Kim Y.J. Effects of cryopreservation on phosphatidylserine translocation, intracellular hydrogen peroxide, and DNA integrity in canine sperm. Theriogenology 2010, 73:282-292.
25. Kim S.H., Lee Y.J. Changes in sperm membrane and ROS following cryopreservation of liquid boar semen stored at 15°C. Anim. Reprod. Sci. 2011, 124:118-124.
26. Lee J.A., Spidlen J., Boyce K., Cai J., Crosbie N., Dalphin M., Furlong J., Gasparetto M., et al. MIFlowCyt: the minimum information about a flow cytometry experiment. Cytometry A 2008, 73:926-930.
27. Lopes S., Jurisicova A., Sun J.G., Casper R.F. Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa. Hum. Reprod. 1998, 13:896-900.
28. Nagy S., Jansen J., Topper E.K., Gadella B.M. A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol. Reprod. 2003, 68:1828-1835.
29. Nasr-Esfahani M.H., Razavi S., Mozdarani H., Mardani M., Azvagi H. Relationship between protamine deficiency with fertilization rate and incidence of sperm premature chromosomal condensation post-ICSI. Andrologia 2004, 36:95-100.
30. Park C.S., Yi Y.J. Comparison of semen characteristics, sperm freezability and testosterone concentration between Duroc and Yorkshire boars during seasons. Anim. Reprod. Sci. 2002, 73:53-61.
31. Peña F.J., Saravia F., Núñez-Martínez I., Johannisson A., Wallgren M., Rodríguez-Martínez H. Do different portions of the boar ejaculate vary in their ability to sustain cryopreservation?. Anim. Reprod. Sci. 2006, 93:101-113.
32. Petrunkina A.M., Waberski D., Bollwein H., Sieme H. Identifying non-sperm particles during flow cytometric physiological assessment: a simple approach. Theriogenology 2010, 73:995-1000.
33. Pursel V.G., Johnson L.A. Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 1975, 40:99-102.
34. Thurston L.M., Siggins K., Mileham A.J., Watson P.F., Holt W.V. Identification of amplified restriction fragment length polymorphism markers linked to genes controlling boar sperm viability following cryopreservation. Biol. Reprod. 2002, 66:545-554.
35. Vilagran I., Castillo J., Bonet S., Sancho S., Yeste M., Estanyol J.M., Oliva R. Acrosin-binding protein (ACRBP) and Triosephosphate isomerase (TPI) are good markers to predict boar sperm freezing capacity. Theriogenology 2013, 80:443-450.
36. Ward M.A., Ward W.S. A model for the function of sperm DNA degradation. Reprod. Fertil. Dev. 2004, 16:547-554.
37. Waterhouse K.E., Hofmo P.O., Tverdal A., Miller R.R. Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm. Reproduction 2006, 131:887-894.
38. Watson P.F. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 2000, 60:481-492.
39. Westendorf P., Richter L., Treu H., von Ebersperma Zur Tiefgefrierung Labor und Besamungsergebnisse mit dem Hülsenberger Pailletten Verfahren [Deep freezing of boar sperm. Laboratory and insemination results using the Hülsenberger paillete method]. Dtsch. Tierärztl. Wschr. 1975, 82:261-267.
40. α to the short-term extender over cooling time. Anim. Reprod. Sci. 2008, 108:180-195.
41. Yeste M., Flores E., Estrada E., Bonet S., Rigau T., Rodríguez-Gil J.E. Reduced glutathione and procaine hydrochloride protect the nucleoprotein structure of boar spermatozoa during freeze-thawing through stabilisation of disulphide bonds. Reprod. Fertil. Dev. 2013, 25:1036-1050.
42. Yeste M., Estrada E., Casas I., Bonet S., Rodríguez-Gil J.E. Good and bad freezability boar ejaculates differ in the integrity of nucleoprotein structure after freeze-thawing but not in ROS levels. Theriogenology 2013, 79:929-939.
43. Zhang W., Yi K., Chen C., Hou X., Zhou X. Application of antioxidants and centrifugation for cryopreservation of boar spermatozoa. Anim. Reprod. Sci. 2012, 132:123-128.