A multiplex two-color real-time PCR method for quality-controlled molecular diagnostic testing of FFPE samples

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Yeo, Jiyoun ^a   Crawford, Erin L ^a   Blomquist, Thomas M ^a   Stanoszek, Lauren M ^a   Dannemiller, Rachel E ^a   Zyrek, Jill ^a   De Las Casas, Luis E ^a   Khuder, Sadik A ^a   Willey, James C ^a  

^a MEDICAL COLLEGE OF OHIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FORMALDEHYDE; PARAFFIN;

ARTICLE; CANCER DIAGNOSIS; CLINICAL ARTICLE; CONTROLLED STUDY; COPY NUMBER VARIATION; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DIAGNOSTIC VALUE; EMBEDDING; FALSE NEGATIVE RESULT; FLUORESCENCE; FLUOROMETRY; FORMALIN FIXED PARAFFIN EMBEDDED; GENE AMPLIFICATION; GENE EXPRESSION; GENE PROBE; GENETIC IDENTIFICATION; GENETIC VARIABILITY; HUMAN; HUMAN CELL; HUMAN TISSUE; HYDROLYSIS; LUNG CANCER; LUNG CANCER DIAGNOSTIC TEST; MOLECULAR DIAGNOSIS; NUCLEOTIDE SEQUENCE; ONCOGENE; ONCOGENE ACTB; ONCOGENE CDKN1A; ONCOGENE E2F1; ONCOGENE MYC; POISSON DISTRIBUTION; PROCESS OPTIMIZATION; QUALITY CONTROL; REAL TIME POLYMERASE CHAIN REACTION; SIGNAL TO ANALYTE RESPONSE; SIGNAL TRANSDUCTION; STANDARD; VALIDATION PROCESS; MULTIPLEX POLYMERASE CHAIN REACTION; PROCEDURES; TISSUE FIXATION;

HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; PARAFFIN EMBEDDING; REAL-TIME POLYMERASE CHAIN REACTION; TISSUE FIXATION;

EID: 84896781624     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0089395     Document Type: Article

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