Robust and artifact-free mounting of tissue samples for atomic force microscopy

BioTechniques

Volumn 56, Issue 1, 2014, Pages 40-42

  Morgan, Joshua T ^a   Raghunathan, Vijay Krishna ^a   Thomasy, Sara M ^a   Murphy, Christopher J ^a,b   Russell, Paul ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Atomic force microscopy; Cornea; Sample preparation; Tissue mechanics; Trabecular meshwork

Indexed keywords

SILICONE; TISSUE ADHESIVE;

ARTICLE; ARTIFACT REDUCTION; ATOMIC FORCE MICROSCOPE; ATOMIC FORCE MICROSCOPY; CORNEA; GLUE IMMOBILIZATION METHOD; HUMAN; HYDRATION; MICROSCOPE; NONHUMAN; PUNCH BIOPSY; SOFT CLAMPING IMMOBILIZING RETAINER OF TISSUE; TISSUE FIXATION; TISSUE INJURY; TRABECULAR MESHWORK;

ATOMIC FORCE MICROSCOPY; CORNEA; SAMPLE PREPARATION; TISSUE MECHANICS; TRABECULAR MESHWORK;

ANIMALS; ARTIFACTS; HUMANS; MICROSCOPY, ATOMIC FORCE; RABBITS; SPECIMEN HANDLING;

EID: 84896732075     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000114126     Document Type: Article

References (14)

1. Discher, D.E., P. Janmey, and Y.L. Wang. 2005. Tissue cells feel and respond to the stiffness of their substrate. Science 310:1139-1143.
2. Yeung, T., P.C. Georges, L.A. Flanagan, B. Marg, M. Ortiz, M. Funaki, N. Zahir, W. Ming, et al. 2005. Effects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion. Cell Motil. Cytoskeleton 60:24-34.
3. Thomasy, S.M., J.A. Wood, P.H. Kass, C.J. Murphy, and P. Russell. 2012. Substratum stiffness and latrunculin B regulate matrix gene and protein expression in human trabecular meshwork cells. Invest. Ophthalmol. Vis. Sci. 53:952-958.
4. McKee, C.T., J.A. Last, P. Russell, and C.J. Murphy. 2011. Indentation versus tensile measurements of Young's modulus for soft biological tissues. Tissue Eng. Part B Rev. 17:155-164.
5. Ebenstein, D.M. and L.A. Pruitt. 2006. Nanoindentation of biological materials. Nano Today 1:26-33.
6. Oyen, M.L. 2011. Nanoindentation of Biological and Biomimetic Materials. Exp. Techniques 37:73-87.
7. Haut, T.L. and R.C. Haut. 1997. The state of tissue hydration determines the strain-rate-sensitive stiffness of human patellar tendon. J. Biomech. 30:79-81.
8. Constantinides, G., Z.I. Kalcioglu, M. McFarland, J.F. Smith, and K.J. Van Vliet. 2008. Probing mechanical properties of fully hydrated gels and biological tissues. J. Biomech. 41:3285-3289.
9. Radmacher, M. 1997. Measuring the elastic properties of biological samples with the AFM. IEEE Eng. Med. Biol. Mag. 16:47-57.
10. Chaurasia, S.S., R. Champakalakshmi, A. Li, R. Poh, X.W. Tan, R. Lakshminarayanan, C.T. Lim, D.T. Tan, and J.S. Mehta. 2012. Effect of fibrin glue on the biomechanical properties of human Descemet's membrane. PLoS ONE 7:e37456.
11. Last, J.A., T. Pan, Y. Ding, C.M. Reilly, K. Keller, T.S. Acott, M.P. Fautsch, C.J. Murphy, and P. Russell. 2011. Elastic modulus determination of normal and glaucomatous human trabecular meshwork. Invest. Ophthalmol. Vis. Sci. 52:2147-2152.
12. Thomasy, S.M., V.K. Raghunathan, M. Winkler, C.M. Reilly, A.R. Sadeli, P. Russell, J.V. Jester, and C.J. Murphy. Elastic modulus and collagen organization of the rabbit cornea: epithelium to endothelium. Acta Biomater. (In press).
13. Last, J.A., S.J. Liliensiek, P.F. Nealey, and C.J. Murphy. 2009. Determining the mechanical properties of human corneal basement membranes with atomic force microscopy. J. Struct. Biol. 167:19-24.
14. Last, J.A., S.M. Thomasy, C.R. Croasdale, P. Russell, and C.J. Murphy. 2012. Compliance profile of the human cornea as measured by atomic force microscopy. Micron 43:1293-1298.