Novel assay for the detection and monitoring of levamisole resistance in Haemonchus contortus

International Journal for Parasitology

Volumn 44, Issue 3-4, 2014, Pages 235-241

  Barrère, Virginie ^a   Beech, Robin N ^a,b   Charvet, Claude L ^b,c   Prichard, Roger K ^a  

^a MCGILL UNIVERSITY   (Canada)

^b UR1282   (France)

^c UNIVERSITÉ DE TOURS   (France)

Author keywords

Genetic marker; Haemonchus contortus; Hco acr 8; Hco acr 8b; Levamisole resistance

Indexed keywords

CHOLINERGIC RECEPTOR; GENOMIC DNA; LEVAMISOLE; ANTINEMATODAL AGENT; GENETIC MARKER; HELMINTH DNA; HELMINTH PROTEIN; RNA SPLICING;

DNA; DRUG RESISTANCE; GENE EXPRESSION; GENETIC MARKER; GENOME; MONITORING; NEMATODE; PROTEIN;

ANTHELMINTHIC RESISTANCE; ANTHELMINTHIC SENSITIVITY; ARTICLE; BINDING SITE; CONTROLLED STUDY; CORRELATION ANALYSIS; DNA DETERMINATION; DNA MARKER; DNA SEQUENCE; DRUG RESISTANCE; DRUG SENSITIVITY; EXON; GENE DELETION; GENE INSERTION; HAEMONCHUS CONTORTUS; INTRON; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; AMINO ACID SEQUENCE; ANIMAL; DRUG EFFECTS; GENE; GENETIC MARKER; GENETICS; HAEMONCHUS; INDEL MUTATION; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; RNA SPLICING;

ANIMALIA; HAEMONCHUS CONTORTUS; NEMATODA;

AMINO ACID SEQUENCE; ANIMALS; ANTINEMATODAL AGENTS; BASE SEQUENCE; DNA, HELMINTH; DRUG RESISTANCE; GENES, HELMINTH; GENETIC MARKERS; HAEMONCHUS; HELMINTH PROTEINS; INDEL MUTATION; LEVAMISOLE; MALE; MOLECULAR SEQUENCE DATA; RECEPTORS, CHOLINERGIC; RNA SPLICE SITES;

EID: 84896725922     PISSN: 00207519     EISSN: 18790135     Source Type: Journal    
DOI: 10.1016/j.ijpara.2013.12.004     Document Type: Article

References (16)

1. Boulin T., Fauvin A., Charvet C.L., Cortet J., Cabaret J., Bessereau J.L., Neveu C. Functional reconstitution of Haemonchus contortus acetylcholine receptors in Xenopus oocytes provides mechanistic insights into levamisole resistance. Br. J. Pharmacol. 2011, 164:1421-1432.
2. De Klein A., Riegman P.H.J., Bijlsma E.K., Heldoorn A., Muijtjens M., den Bakker M.A., Avezaat C.J.J., Zwarthoff E.C. A G→A transition creates a branch point sequence and activation of a cryptic exon, resulting in the hereditary disorder neurofibromatosis 2. Hum. Mol. Genet. 1997, 7:393-398.
3. Fauvin A., Charvet C., Issouf M., Cortet J., Cabaret J., Neveu C. CDNA-AFLP analysis in levamisole-resistant Haemonchus contortus reveals alternative splicing in a nicotinic acetylcholine receptor subunit. Mol. Biochem. Parasitol. 2010, 170:105-107.
4. Hernando G., Berge I., Rayes D., Bouzat C. Contribution of subunits to Caenorhabditis elegans levamisole-sensitive nicotinic receptor function. Mol. Pharmacol. 2012, 82:550-560.
5. Hoekstra R., Borgsteede F.H., Boersema J.H., Roos M.H. Selection for high levamisole resistance in Haemonchus contortus monitored with an egg-hatch assay. Int. J. Parasitol. 1997, 27:1395-1400.
6. Kopp S.R., Coleman G.T., Traub R.J., McCarthy J.S., Kotze A.C. Acetylcholine receptor subunit genes from Ancylostoma caninum: altered transcription patterns associated with pyrantel resistance. Int. J. Parasitol. 2009, 39:435-441.
7. Laing R., Kikuchi T., Martinelli A., Tsai I.J., Beech R.N., Redman E., Holroyd N., Bartley D.J., Beasley H., Britton C., Curran D., Devaney E., Gilabert A., Hunt M., Jackson F., Johnston S.L., Kryukov I., Li K., Morrison A.A., Reid A.J., Sargison N., Saunders G.I., Wasmuth J.D., Wolstenholme A., Berriman M., Gilleard J.S., Cotton J.A. The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery. Genome Biol. 2013, 14:R88.
8. Neveu C., Charvet C., Fauvin A., Cortet J., Beech R.N., Cabaret J. Genetic diversity of levamisole receptor subunits in parasitic nematode species and abbreviated transcripts associated with resistance. Pharmacol. Genom. 2010, 20:414-425.
9. Otsen M., Hoekstra R., Plas M.E., Buntjer J.B., Lenstra J.A., Roos M.H. Amplified fragment length polymorphism analysis of genetic diversity of Haemonchus contortus during selection for drug resistance. Int. J. Parasitol. 2001, 31:1138-1143.
10. Ranjan S., Wang G., Hirschlein C., Simkins K. Selection for resistance to macrocyclic lactones by Haemonchus contortus in sheep. Vet. Parasitol. 2002, 103:109-117.
11. Roos M.H., Otsen M., Hoekstra R., Veenstra J.G., Lenstra J.A. Genetic analysis of inbreeding of two strains of the parasitic nematode Haemonchus contortus. Int. J. Parasitol. 2004, 34:109-115.
12. Rufener L., Mäser P., Roditi I., Kaminsky R. Haemonchus contortus acetylcholine receptors of the DEG-3 subfamily and their role in sensitivity to monepantel. PLoS Pathog. 2009, 5:e1000380.
13. Sarai R.S., Kopp S.R., Coleman G.T., Kotze A.C. Acetylcholine receptor subunit and P-glycoprotein transcription patterns in levamisole-susceptible and -resistant Haemonchus contortus. Int. J. Parasitol.: Drug Drug Res. 2013, 3:51-58.
14. Schwarz E.M., Korhonen P.K., Campbell B.E., Young N.D., Jex A.R., Jabbar A., Hall R.S., Mondal A., Howe A.C., Pell J., Hofmann A., Boag P.R., Zhu X.Q., Gregory T.R., Loukas A., Williams B.A., Antoshechkin I., Brown C.T., Sternberg P.W., Gasser R.B. The genome and developmental transcriptome of the strongylid nematode Haemonchus contortus. Genome Biol. 2013, 14:R89.
15. van Wyk J.A., Malan F.S., Randles J.L. How long before resistance makes it impossible to control some field strains of Haemonchus contortus in South Africa with any of the modern anthelmintics?. Vet. Parasitol. 1997, 70:111-122.
16. Williamson S.M., Storey B., Howell S., Harper K.M., Kaplan R.M., Wolstenholme A.J. Candidate anthelmintic resistance-associated gene expression and sequence polymorphisms in a triple-resistant field isolate of Haemonchus contortus. Mol. Biochem. Parasitol. 2011, 180:99-105.