Luminescence enhancement of the catalytic 19 kDa protein (KAZ) of Oplophorus luciferase by three amino acid substitutions

Biochemical and Biophysical Research Communications

Volumn 445, Issue 1, 2014, Pages 157-162

  Inouye, Satoshi ^a   Sato, Jun Ichi ^a   Sahara Miura, Yuiko ^a   Yoshida, Suguru ^b   Hosoya, Takamitsu ^b  

^a CHISSO CORPORATION   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Coelenterazine analogues; Inclusion bodies; Mutagenesis; Secretion

Indexed keywords

AMINO ACID; KAZ PROTEIN; LUCIFERASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; IN VITRO STUDY; LUMINESCENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN SECRETION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; OPLOPHORUS;

COELENTERAZINE ANALOGUES; INCLUSION BODIES; MUTAGENESIS; SECRETION;

AMINO ACID SUBSTITUTION; ANIMALS; BIOCATALYSIS; CHO CELLS; CRICETINAE; CRICETULUS; DECAPODA (CRUSTACEA); ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; IMIDAZOLES; LUCIFERASES; LUMINESCENCE; LUMINESCENT MEASUREMENTS; MOLECULAR STRUCTURE; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PYRAZINES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

EID: 84896724214     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2014.01.133     Document Type: Article

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