C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: The mutated catalytic 19kDa component of Oplophorus luciferase

Biochemical and Biophysical Research Communications

Volumn 437, Issue 1, 2013, Pages 23-28

  Inouye, Satoshi ^a   Sato, Jun ichi ^a   Sahara Miura, Yuiko ^a   Yoshida, Suguru ^b   Kurakata, Hajime ^c   Hosoya, Takamitsu ^b  

^a CHISSO CORPORATION   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Codon optimized gene; Coelenterazine; Furimazine; Gaussia; Reporter assay

Indexed keywords

LUCIFERASE; NANOKAZ PROTEIN; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CODON; COELENTERATE; CONTROLLED STUDY; ENZYME SPECIFICITY; ESCHERICHIA COLI; LUMINESCENCE; NONHUMAN; OPLOPHORUS; PRIORITY JOURNAL; PROTEIN PURIFICATION;

19KOLASE; CODON-OPTIMIZED GENE; COELENTERAZINE; CTZ; FULL WIDTH AT HALF MAXIMUM; FURIMAZINE; FWHM; GAUSSIA; GAUSSIA LUCIFERASE; GLASE; I(MAX); MAXIMUM INTENSITY OF LUMINESCENCE; NANOKAZ; RELATIVE LIGHT UNITS; REPORTER ASSAY; RLU; THE CATALYTIC 19KDA PROTEIN OF OPLOPHORUS LUCIFERASE; THE MUTATED 19KOLASE;

AMINO ACID SEQUENCE; ANIMALS; CATALYTIC DOMAIN; CHO CELLS; COPEPODA; CRICETINAE; CRICETULUS; DECAPODA (CRUSTACEA); ESCHERICHIA COLI; IMIDAZOLES; LUCIFERASES; LUMINESCENCE; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; PROTEIN SORTING SIGNALS; PYRAZINES; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; OPLOPHORUS;

EID: 84880513244     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2013.06.026     Document Type: Article

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