Extreme rapid warming yields high functional survivals of vitrified 8-cell mouse embryos even when suspended in a half-strength vitrification solution and cooled at moderate rates to -196°C

Cryobiology

Volumn 68, Issue 1, 2014, Pages 71-78

  Seki, Shinsuke ^a,b   Jin, Bo ^a   Mazur, Peter ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

^b TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Cooling rates; Embryos; Mouse; Oocytes; Solute concentration in media; Survival; Vitrification; Warming rates

Indexed keywords

ANALYSIS OF VARIANCE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTOCYST; CELL SURVIVAL; CELL VIABILITY; CRYOPRESERVATION; CRYSTALLIZATION; CYTOLOGY; EMBRYO; FEMALE; IONIZATION; MALE; MEIOSIS; MORPHOLOGY; MOUSE; NONHUMAN; OOCYTE; OSMOLALITY; OSMOSIS; PRIORITY JOURNAL; THAWING; THERMODYNAMICS; VISCOSITY; VITRIFICATION; WARMING; ANIMAL EXPERIMENT; CONTROLLED STUDY; COOLING; EMBRYO DEVELOPMENT; OSMOTIC FRAGILITY; SURVIVAL RATE; TISSUE STRUCTURE; TISSUE SURVIVAL;

COOLING RATES; EMBRYOS; MOUSE; OOCYTES; SOLUTE CONCENTRATION IN MEDIA; SURVIVAL; VITRIFICATION; WARMING RATES;

ANIMALS; CELL SURVIVAL; CRYOPRESERVATION; EMBRYO, MAMMALIAN; EMBRYONIC DEVELOPMENT; FEMALE; MALE; MICE; ORGAN PRESERVATION SOLUTIONS; OSMOSIS; TIME FACTORS; VITRIFICATION;

EID: 84896695433     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2013.12.001     Document Type: Article

References (26)

1. Kleinhans F.W., Seki S., Mazur P. Simple inexpensive attainment and measurement of very high cooling and warming rates. Cryobiology 2010, 61:231-233.
2. Larman M.G., Sheehan C.B., Gardner D.K. Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction 2006, 131:53-61.
3. Leibo S.P., McGrath J.J., Cravalho E.G. Microscopic observation of intracellular ice formation in unfertilized mouse ova as a function of cooling rate. Cryobiology 1978, 15:257-271.
4. Liu X.H., Zhang T., Rawson D.M. Effect of cooling rate and partial removal of yolk on the chilling injury in zebrafish (Danio rerio) embryos. Theriogenology 2001, 55:1719-1731.
5. Mazur P. Kinetics of water loss from cells at subzero temperature and the likelihood of intracellular freezing. J. Gen. Physiol. 1963, 47:347-369.
6. Mazur P., Pinn I.L., Kleinhans F.W. Intracellular ice formation in mouse oocytes subjected to interrupted rapid cooling. Cryobiology 2007, 55:158-166.
7. Mazur P., Schneider U., Mahowald A.P. Characteristics and kinetics of subzero chilling injury in Drosophila embryos. Cryobiology 1992, 29:39-68.
8. Mazur P., Seki S., Pinn I.L., Kleinhans F.W., Edashige K. Extra- and intracellular ice formation in mouse oocytes. Cryobiology 2005, 51:29-53.
9. Mazur P., Seki S. Survival of mouse oocytes after being cooled in a vitrification solution to -196°C at 95 to 70,000°C/min and warmed at 610 to 118,000°C/min: a new paradigm for cryopreservation by vitrification. Cryobiology 2011, 62:1-7.
10. Paredes E., Mazur P. The survival of mouse oocytes shows little or no correlation with the vitrification or freezing of the external medium, but the ability of the medium to vitrify is affected by its solute concentration and by the cooling rate. Cryobiology 2013, 67:386-390.
11. P.B. Pedro, Studies on the cryopreservation of mammalian oocytes and embryos with reference to some cryobiological characteristics (Ph.D. thesis). Ehime University, Japan, 2003 (Chapter 3).
12. Pedro P.B., Yokoyama E., Zhu S.E., Yoshida N., Valdez D.M., Tanaka M., Edashige K., Kasai M. Permeability of mouse oocytes and embryos at various developmental stages to five cryoprotectants. J. Reprod. Dev. 2005, 51:235-246.
13. Pedro P.B., Zhu S.E., Makino N., Sakurai T., Edashige K., Kasai M. Effects of hypotonic stress on the survival of mouse oocytes and embryos at various stages. Cryobiology 1997, 35:150-158.
14. Pollard J.W., Leibo S.P. Chilling sensitivity of mammalian embryos. Theriogenology 1994, 41:101-106.
15. Rall W.F. Factors affecting the survival of mouse embryos cryopreserved by vitrification. Cryobiology 1987, 24:387-402.
16. Rall W.F., Mazur P., McGrath J.J. Depression of the ice-nucleation temperature of rapidly cooled mouse embryos by glycerol and dimethyl sulfoxide. Biophys. J. 1983, 41:1-12.
17. Seki S., Kouya T., Tsuchiya R., Valdez D.M., Jin B., Koshimoto C., Kasai M., Edashige K. Cryobiological properties of immature zebrafish oocytes assessed by their ability to be fertilized and develop into hatching embryos. Cryobiology 2011, 62:8-14.
18. Seki S., Mazur P. Effect of warming rate on the survival of vitrified mouse oocytes and on the recrystallization of intracellular ice. Biol. Reprod. 2008, 79:727-737.
19. Seki S., Mazur P. Kinetics and activation energy of recrystallization of intracellular ice in mouse oocytes subjected to a interrupted rapid cooling. Cryobiology 2008, 56:171-180.
20. Seki S., Mazur P. The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology 2009, 59:75-82.
21. Seki S., Mazur P. Ultra-rapid warming yields high survival of mouse oocytes cooled to -196°C in dilutions of a standard vitrification solution. PLoS One 2012, 7:e36058.
22. Songsasen N., Yu I., Ratterree M., VandeVoort C., Leibo S.P. Effect of chilling on the organization of tubulin and chromosomes in rhesus monkey oocytes. Fertil. Steril. 2002, 77:818-825.
23. Tsai S., Rawson D.M., Zhang T. Studies on chilling sensitivity of early stage zebrafish (Danio rerio) ovarian follicles. Cryobiology 2009, 58:279-286.
24. Whittingham D.G., Leibo S.P., Mazur P. Survival of mouse embryos frozen to -196 and -269°C. Science 1972, 178:411-414.
25. Yavin S., Aroyo A., Roth Z., Arav A. Embryo cryopreservation in the presence of low concentration of vitrification solution with sealed pulled straws in liquid nitrogen slush. Hum. Reprod. 2009, 24:797-804.
26. Zenzes M.T., Bieleicki R., Casper R., Leibo S.P. Effects of chilling to 0°C on the morphology of meiotic spindles in human metaphase II oocytes. Fertil. Steril. 2001, 75:769-777.