Simple, inexpensive attainment and measurement of very high cooling and warming rates

Cryobiology

Volumn 61, Issue 2, 2010, Pages 231-233

  Kleinhans, F W ^a,b   Seki, Shinsuke ^a   Mazur, Peter ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Cooling; Cryotop; High rate; Inexpensive temperature measurement; Type T thermocouple; USB oscilloscope; Warming

Indexed keywords

ICE; METAL; POLYPROPYLENE;

AQUEOUS SOLUTION; ARTICLE; CONTROLLED STUDY; COOLING; COST; DATA ANALYSIS; ELECTRIC POTENTIAL; MINICOMPUTER; OSCILLOSCOPE; PRIORITY JOURNAL; TEMPERATURE MEASUREMENT; TEMPERATURE SENSITIVITY; THERMOCOUPLE; THERMOMETER; WARMING;

COLD TEMPERATURE; COMPUTERS; DATA COLLECTION; EQUIPMENT DESIGN; FREEZING; HOT TEMPERATURE; THERMOMETERS;

EID: 77956937779     PISSN: 00112240     EISSN: 10902392     Source Type: Journal    
DOI: 10.1016/j.cryobiol.2010.06.011     Document Type: Article

References (8)

1. EasySync Ltd., Hillsboro, Oregon, USA,http://www.easysync-ltd.com/.
2. Kitazato Biopharma, Japan,http://www.kitazato-biopharma.com/cryotop/method.html.
3. Kleinhans F.W., Seki S., Mazur P. Abstract presented at Cryo-2009: simple, inexpensive measurement of very rapid cooling and warming rates. Cryobiology 2009, 59:381.
4. Kuwayama M. Highly efficient vitrification method for cryopreservation of human oocytes. Reproductive BioMedicine Online 2005, 11:300-308.
5. Mazur P., Seki S., Kleinhans F.W. Abstract presented at Cryo-2009: survival of mouse oocytes suspended in EAFS 10/10 vitrification solution after being cooled to -196°C on Cryotops at rates ranging from 95°C/min to 70,000°C/min and warmed at 610°C/min to 118,000°C/min. Cryobiology 2009, 59:396.
6. Omega Engineering Inc., Stamford, Connecticut, USA,The 50μm wire Type T thermocouple is part # COCO-002. I.e. Copper-Constantan 0.002" wire thermocouple. http://www.omega.com/.
7. Omega Engineering Inc., Stamford, Connecticut, USA. These response data appear on the web order page for " Unsheathed Fine Gage Microtemp Thermocouples, J, K, T, E, R &S" ; specifically,http://www.omega.com/ppt/pptsc.asp?ref=IRCO_CHAL_P13R_P10R&Nav=tema02.
8. Seki S., Mazur P. The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology 2009, 59:75-82.