Acute appendicitis in children is associated with an abundance of bacteria from the phylum Fusobacteria

Journal of Pediatric Surgery

Volumn 49, Issue 3, 2014, Pages 441-446

  Zhong, Diana ^a   Brower Sinning, Rachel ^a,b   Firek, Brian ^a,b   Morowitz, Michael J ^a,b  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b CHILDREN S HOSPITAL OF PITTSBURGH   (United States)

Author keywords

Appendicitis; Fusobacteria; Gut bacteria; Microbiome

Indexed keywords

RNA 16S; BACTERIAL DNA; BACTERIAL RNA; RIBOSOME DNA;

ACUTE APPENDICITIS; ADOLESCENT; ADULT; APPENDECTOMY; APPENDIX PERFORATION; ARTICLE; BACTERIUM CULTURE; BACTEROIDES; CLINICAL ARTICLE; DNA SEQUENCE; FUSOBACTERIUM; FUSOBACTERIUM NUCLEATUM; GEMELLA; GENE SEQUENCE; HUMAN; MICROBIAL COMMUNITY; PARVIMONAS; PILOT STUDY; POPULATION ABUNDANCE; PORPHYROMONAS; PREVOTELLA; PRIORITY JOURNAL; RNA GENE; YOUNG ADULT; APPENDICITIS; APPENDIX; BODY FLUID; CHILD; GENETICS; INFANT; ISOLATION AND PURIFICATION; MICROBIOLOGY; MICROFLORA; MOUTH; PRESCHOOL CHILD; PROTEOBACTERIA; RIBOTYPING;

ADOLESCENT; APPENDECTOMY; APPENDICITIS; APPENDIX; BACTEROIDES; BODY FLUIDS; CHILD; CHILD, PRESCHOOL; DNA, BACTERIAL; DNA, RIBOSOMAL; FUSOBACTERIUM; HUMANS; INFANT; MICROBIOTA; MOUTH; PILOT PROJECTS; PORPHYROMONAS; PROTEOBACTERIA; RIBOTYPING; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

EID: 84896522056     PISSN: 00223468     EISSN: 15315037     Source Type: Journal    
DOI: 10.1016/j.jpedsurg.2013.06.026     Document Type: Article

References (28)

1. B.A. Birnbaum, and S.R. Wilson Appendicitis at the millennium 1 Radiology 215 2 2000 337 348
2. Lamps LW. Infectious causes of appendicitis. Infect. Dis. Clin. North Am. 2010;24(4):995-1018-ix-x.
3. R. Andersson, A. Hugander, and A. Thulin et al. Clusters of acute appendicitis: further evidence for an infectious aetiology Int J Epidemiol 24 4 1995 829 833
4. Y. Deng, D.C. Chang, and Y. Zhang et al. Seasonal and day of the week variations of perforated appendicitis in US children Pediatr Surg Int 26 7 2010 691 696 Springer-Verlag
5. Bickler SW, DeMaio A. Western diseases: current concepts and implications for pediatric surgery research and practice. Pediatr Surg Int. Springer-Verlag; 2007;24(3):251-5
6. Salipante SJ, Sengupta DJ, Rosenthal C, et al. Rapid 16S rRNA next-generation sequencing of polymicrobial clinical samples for diagnosis of complex bacterial infections. PLoS ONE. Public Library of Science; 2013;8(5):e65226
7. M. Hamady, and R. Knight Microbial community profiling for human microbiome projects: tools, techniques, and challenges Genome Res 19 7 2009 1141 1152
8. Chen C-Y, Chen Y-C, Pu H-N, et al. Bacteriology of acute appendicitis and its implication for the use of prophylactic antibiotics. Surgical infections. Mary Ann Liebert, Inc. 140 Huguenot Street, 3rd Floor New Rochelle, NY 10801 USA; 2012;13(6):383-90.
9. C. Guillet-Caruba, A. Cheikhelard, and M. Guillet et al. Bacteriologic epidemiology and empirical treatment of pediatric complicated appendicitis Diagn Microbiol Infect Dis 69 4 2011 376 381
10. Roberts JP. Quantitative bacterial flora of acute appendicitis. Archives of Disease in Childhood. BMJ Group; 1988;63(5):536.
11. A. Swidsinski, Y. Dorffel, and V. Loening-Baucke et al. Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum Gut 60 1 2010 34 40
12. A. Swidsinski, V. Loening-Baucke, and S. Biche-ool et al. Mucosal invasion by fusobacteria is a common feature of acute appendicitis in Germany, Russia, and China Saudi J Gastroenterol 18 1 2012 55
13. Guinane CM, Tadrous A, Fouhy F, et al. Microbial composition of human appendices from patients following appendectomy. mBio. 2012;4(1):e00366-12- e00366-12.
14. J.G. Caporaso, J. Kuczynski, and J. Stombaugh et al. QIIME allows analysis of high-throughput community sequencing data Nat Meth 7 5 2010 335 336
15. J.R. Cole, Q. Wang, and E. Cardenas et al. The Ribosomal Database Project: improved alignments and new tools for rRNA analysis Nucleic Acids Res 37 Suppl 1 2009 D141 D145
16. Lozupone CA, Stombaugh JI, Gordon JI, et al. Diversity, stability and resilience of the human gut microbiota. Nature. Nature Publishing Group; 2012;489(7415):220-30
17. N. Segata, S.K. Haake, and P. Mannon et al. Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples Genome Biol BioMed Central Ltd; 13 6 2012 R42
18. Taur Y, Xavier JB, Lipuma L, et al. Intestinal domination and the risk of bacteremia in patients undergoing allogeneic hematopoietic stem cell transplantation. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America [Internet]. 2012 Jun 20. Available from: http://cid.oxfordjournals.org/lookup/doi/10.1093/cid/cis580.
19. C. Lozupone, M.E. Lladser, and D. Knights et al. UniFrac: an effective distance metric for microbial community comparison ISME J 5 2 2010 169 172
20. E. Jumas-Bilak, J.P. Carlier, and H. Jean-Pierre et al. Jonquetella anthropi gen. nov., sp. nov., the first member of the candidate phylum "Synergistetes" isolated from man Int J Syst Evol Microbiol 57 12 2007 2743 2748
21. Owen H. Wangensteen CD. Experimental proof of the obstructive origin of appendicitis in man. Annals of Surgery. Lippincott, Williams, and Wilkins; 1939; 110(4):629.
22. E. Arnbjörnsson, and S. Bengmark Role of obstruction in the pathogenesis of acute appendicitis Am J Surg 147 3 1984 390 392 (Pubitemid 14154593)
23. Carr NJ. The pathology of acute appendicitis. Annals of diagnostic pathology. Elsevier; 2000;4(1):46-58.
24. M. Laurin, M.L. Everett, and W. Parker The cecal appendix: one more immune component with a function disturbed by post-industrial culture Anat Rec 294 4 2011 567 579
25. K.K. Varadhan, K.R. Neal, and D.N. Lobo Safety and efficacy of antibiotics compared with appendicectomy for treatment of uncomplicated acute appendicitis: meta-analysis of randomised controlled trials BMJ 344 apr05 1 2012 e2156 e2160
26. Y.W. Han, and X. Wang Mobile microbiome: oral bacteria in extra-oral infections and inflammation J Dent Res 92 6 2013 485 491
27. E. Allen-Vercoe, J. Strauss, and K. Chadee Fusobacterium nucleatum: an emerging gut pathogen? Gut Microbes. Landes, Bioscience 2 5 2013 294 298
28. H.R. Lee, I.C. Rhyu, and H.D. Kim et al. In-vivo-induced antigenic determinants of Fusobacterium nucleatum subsp. nucleatum Molec Oral Microbiol 26 2 2011 164 172