Single color FRET based measurements of conformational changes of proteins resulting from translocation inside cells

Methods

Volumn 66, Issue 2, 2014, Pages 180-187

  Gahl, Robert F ^a   Tekle, Ephrem ^a   Tjandra, Nico ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

Author keywords

Apoptosis; Conformational changes; FRET; Live cell imaging; Microinjection; Translocation

Indexed keywords

CYSTEINE; PROTEIN BAX; BAX PROTEIN, HUMAN; FLUORESCENT DYE;

APOPTOSIS; ARTICLE; CELLULAR DISTRIBUTION; CONFORMATIONAL TRANSITION; CYTOSOL; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE ANALYSIS; IN VITRO STUDY; MITOCHONDRIAL MEMBRANE; MITOCHONDRION; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; ANIMAL; CELL CULTURE; CHEMISTRY; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; METABOLISM; MICROINJECTION; MOUSE; PROCEDURES; PROTEIN TRANSPORT; SINGLE CELL ANALYSIS; STANDARD; STANDARDS;

ALEXA;

ANIMALS; BCL-2-ASSOCIATED X PROTEIN; CELLS, CULTURED; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; MICE; MICROINJECTIONS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PROTEIN CONFORMATION; PROTEIN TRANSPORT; REFERENCE STANDARDS; SINGLE-CELL ANALYSIS;

EID: 84896486413     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2013.07.011     Document Type: Article

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