Identification of a novel vga(E) gene variant that confers resistance to pleuromutilins, lincosamides and streptogramin A antibiotics in staphylococci of porcine origin

Journal of Antimicrobial Chemotherapy

Volumn 69, Issue 4, 2014, Pages 919-923

  Li, Jun ^a   Li, Beibei ^b   Wendlandt, Sarah ^c   Schwarz, Stefan ^c   Wang, Yang ^a   Wu, Congming ^a   Ma, Zhiyong ^b   Shen, Jianzhong ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

Author keywords

ABC transporters; Animal staphylococci; Plasmids; Recombination; Transposons

Indexed keywords

ABC TRANSPORTER; AMINO ACID DERIVATIVE; BACTERIAL PROTEIN; CLINDAMYCIN; DALFOPRISTIN; LINCOMYCIN; LINCOSAMIDE DERIVATIVE; PLASMID DNA; PLEUROMUTILIN; PROTEIN VARIANT; RETAPAMULIN; TIAMULIN; UNCLASSIFIED DRUG; VALNEMULIN; VGA (E) PROTEIN;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CHINA; CONTROLLED STUDY; CROSS RESISTANCE; ELECTROTRANSFORMATION; GENE IDENTIFICATION; GENE LOCATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; PROTEIN ANALYSIS; SHUTTLE VECTOR; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS COHNII; STAPHYLOCOCCUS HYICUS; STAPHYLOCOCCUS SIMULANS; SWINE; VGA (E) GENE;

ABC TRANSPORTERS; ANIMAL STAPHYLOCOCCI; PLASMIDS; RECOMBINATION; TRANSPOSONS;

ANIMALS; ANTI-BACTERIAL AGENTS; DITERPENES; DNA, BACTERIAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; GENES, BACTERIAL; LINCOSAMIDES; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PLASMIDS; SEQUENCE ANALYSIS, DNA; STAPHYLOCOCCUS; STREPTOGRAMIN A; SWINE; TRANSFORMATION, BACTERIAL;

EID: 84896485003     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkt482     Document Type: Article

References (23)

1. Kadlec K, Schwarz S. Novel ABC transporter gene, vga(C), located on a multiresistance plasmid from a porcine methicillin-resistant Staphylococcus aureus ST398 strain. Antimicrob Agents Chemother 2009; 53: 3589-91.
2. Jung YH, Shin ES, Kim O et al. Characterization of two newly identified genes, vgaD and vatH, conferring resistance to streptogramin A in Enterococcus faecium. Antimicrob Agents Chemother 2010; 54: 4744-9.
3. Schwendener S, Perreten V. New transposon Tn6133 in methicillinresistant Staphylococcus aureus ST398 contains vga(E), a novel streptogramin A, pleuromutilin, and lincosamide resistance gene. Antimicrob Agents Chemother 2011; 55: 4900-4.
4. Malbruny B, Werno AM, Murdoch DR et al. Cross-resistance to lincosamides, streptogramins A, and pleuromutilins due to the lsa(C) gene in Streptococcus agalactiae UCN70. Antimicrob Agents Chemother 2011; 55: 1470-4.
5. Wendlandt S, Lozano C, Kadlec K et al. The enterococcal ABC transporter gene lsa(E) confers combined resistance to lincosamides, pleuromutilins and streptogramin A antibiotics in methicillin-susceptible and methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 2013; 68: 473-5.
6. Kadlec K, Pomba CF, Couto N et al. Small plasmids carrying vga(A) or vga(C) genes mediate resistance to lincosamides, pleuromutilins and streptogramin A antibiotics in methicillin-resistant Staphylococcus aureus ST398 from swine. J Antimicrob Chemother 2010; 65: 2692-3.
7. Hauschild T, Fessler AT, Kadlec K et al. Detection of thenovel vga(E)genein methicillin-resistant Staphylococcus aureus CC398 isolates from cattle and poultry. J Antimicrob Chemother 2012; 67: 503-4.
8. Monecke S, Ruppelt A, Wendlandt S et al. Genotyping of Staphylococcus aureus isolates from diseased poultry. Vet Microbiol 2013; 162: 806-12.
9. Lozano C, Aspiroz C, Sáenz Y et al. Genetic environmentandlocation of the lnu(A) and lnu(B) genes in methicillin-resistant Staphylococcus aureus and other staphylococci of animal and human origin. J Antimicrob Chemother 2012; 67: 2804-8.
10. Wendlandt S, Li B, Lozano C et al. Identification of the novel spectinomycin resistance gene spw in methicillin-resistant and methicillin-susceptible Staphylococcus aureus of human and animal origin. J Antimicrob Chemother 2013; 68: 1679-80.
11. Li B, Wendlandt S, Yao J et al. Detection andnewgenetic environmentof the pleuromutilin-lincosamide-streptogramin A resistance gene lsa(E) in methicillin-resistant Staphylococcus aureus of swine origin. J Antimicrob Chemother 2013; 68: 1251-5.
12. Wendlandt S, Li B, Ma Z et al. Complete sequence of the multi-resistance plasmid pV7037 from a porcine methicillin-resistant Staphylococcus aureus. Vet Microbiol 2013; 166: 650-4.
13. Allignet J, Loncle V, El Solh N. Sequence of a staphylococcal plasmid gene, vga, encoding a putative ATP-binding protein involved in resistance to virginiamycin A-like antibiotics. Gene 1992; 117: 45-51.
14. Lozano C, Aspiroz C, Rezusta A et al. Identification of novel vga(A)-carrying plasmids and a Tn5406-like transposon in meticillinresistant Staphylococcus aureus and Staphylococcus epidermidis of human and animal origin. Int J Antimicrob Agents 2012; 40: 306-12.
15. Allignet J, El Solh N. Characterization of a new staphylococcal gene, vgaB, encoding a putative ABC transporter conferring resistance to streptogramin A and related compounds. Gene 1997; 202: 133-8.
16. Haroche J, Allignet J, Buchrieser C et al. Characterization of a variant of vga(A) conferring resistance to streptogramin A and related compounds. Antimicrob Agents Chemother 2000; 44: 2271-5.
17. Novotna G, Janata J. A new evolutionary variant of the streptogramin A resistance protein, Vga(A)LC, from Staphylococcus haemolyticus with shifted substrate specificity towards lincosamides. Antimicrob Agents Chemother 2006; 50: 4070-6.
18. Aarestrup FM, Jensen LB. Trends in antimicrobial susceptibility in relation to antimicrobial usage and presence of resistance genes in Staphylococcus hyicus isolated from exudative epidermitis in pigs. Vet Microbiol 2002; 89: 83-94.
19. Haroche J, Allignet J, El Solh N. Tn5406, a new staphylococcal transposon conferring resistance to streptogramin A and related compounds including dalfopristin. Antimicrob Agents Chemother 2002; 46: 2337-43.
20. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals-Fourth Edition: Approved Standard VET01-A4. CLSI, Wayne, PA, USA, 2013.
21. Schneider E, Hunke S. ATP-binding-cassette (ABC) transport systems: functional and structural aspects of the ATP-hydrolyzing subunits/domains. FEMS Microbiol Rev 1998; 22: 1-20.
22. Kadlec K, Fessler AT, Couto N et al. Unusual small plasmids carrying the novel resistance genes dfrK or apmA isolated from methicillin-resistant or -susceptible staphylococci. J Antimicrob Chemother 2012; 67: 2342-5.
23. Brenciani A, Tiberi E, Bacciaglia A et al. Twodistinct genetic elements are responsible for erm(TR)-mediated erythromycin resistance in tetracyclinesusceptible and tetracycline-resistant strains of Streptococcus pyogenes. Antimicrob Agents Chemother 2011; 55: 2106-12.