Protein cell-surface display through in situ enzymatic modification of proteins with a poly(Ethylene glycol)-lipid

Biotechnology and Bioengineering

Volumn 110, Issue 10, 2013, Pages 2785-2789

  Tomita, Urara ^a   Yamaguchi, Satoshi ^a   Maeda, Yasukazu ^a   Chujo, Kazuki ^a   Minamihata, Kosuke ^a   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Enzyme; Fc domain; Mammalian cell; PEG lipid; Phagocytosis; Protein display

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; CELL MEMBRANES; CYTOLOGY; ENZYME ACTIVITY; ENZYMES; GENE TRANSFER; POLYETHYLENE GLYCOLS; SOAPS (DETERGENTS); TISSUE ENGINEERING;

CELL SURFACE DISPLAYS; CELL-CELL INTERACTION; ENZYMATIC MODIFICATION; EXTRACELLULAR MEDIUM; FC DOMAIN; MAMMALIAN CELLS; PHAGOCYTOSIS; RECEPTOR INTERACTION;

PROTEINS;

AMPHOPHILE; FC RECEPTOR; HYBRID PROTEIN; IMMUNOGLOBULIN G; LIPID; MACROGOL 4000; SORTASE; 1,2 DIELAIDOYLPHOSPHATIDYLETHANOLAMINE; 1,2-DIELAIDOYLPHOSPHATIDYLETHANOLAMINE; AMINOACYLTRANSFERASE; BACTERIAL PROTEIN; CYSTEINE PROTEINASE; ENZYME; IMMUNOGLOBULIN FC FRAGMENT; MACROGOL DERIVATIVE; MEMBRANE PROTEIN; PHOSPHATIDYLETHANOLAMINE; SORTASE A;

ARTICLE; CANCER CELL; CATALYSIS; CELL INTERACTION; CELL MEMBRANE; CELL SURFACE DISPLAY; DENDRITIC CELL; ENZYMATIC ASSAY; MOLECULAR RECOGNITION; PHAGOCYTOSIS; PROTEIN AGGREGATION; PROTEIN DOMAIN; PROTEIN MODIFICATION; TISSUE ENGINEERING; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; FC DOMAIN; HELA CELL; HUMAN; MAMMAL CELL; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOUSE; PEG LIPID; PROTEIN DISPLAY; TUMOR CELL LINE; HELA CELL LINE; PROCEDURES;

ENZYME; FC DOMAIN; MAMMALIAN CELL; PEG LIPID; PHAGOCYTOSIS; PROTEIN DISPLAY;

AMINO ACID SEQUENCE; AMINOACYLTRANSFERASES; ANIMALS; BACTERIAL PROTEINS; CELL LINE, TUMOR; CELL SURFACE DISPLAY TECHNIQUES; CYSTEINE ENDOPEPTIDASES; HELA CELLS; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; MEMBRANE PROTEINS; MICE; MOLECULAR SEQUENCE DATA; PHOSPHATIDYLETHANOLAMINES; POLYETHYLENE GLYCOLS;

EID: 84896421681     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24933     Document Type: Article

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