Precise excision of plastid DNA by the large serine recombinase Bxb1

Plant Biotechnology Journal

Volumn 12, Issue 3, 2014, Pages 322-329

  Shao, Min ^a   Kumar, Shashi ^b   Thomson, James G ^c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Italy)

^c WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

Bxb1 recombinase; Chloroplast transformation; Nicotiana tabacum; Site specific recombination; Transgene excision

Indexed keywords

NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

RECOMBINASE; SERINE; VEGETABLE PROTEIN;

ARTICLE; BIOTECHNOLOGY; BXB1 RECOMBINASE; CHLOROPLAST; CHLOROPLAST GENOME; CHLOROPLAST TRANSFORMATION; DNA SEQUENCE; ENZYMOLOGY; GENETIC ENGINEERING; GENETIC MARKER; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY; PLANT LEAF; REPORTER GENE; SITE-SPECIFIC RECOMBINATION; TOBACCO; TRANSGENE; TRANSGENE EXCISION; TRANSGENIC PLANT;

BXB1 RECOMBINASE; CHLOROPLAST TRANSFORMATION; NICOTIANA TABACUM; SITE-SPECIFIC RECOMBINATION; TRANSGENE EXCISION;

BIOTECHNOLOGY; CHLOROPLASTS; GENES, REPORTER; GENETIC ENGINEERING; GENETIC MARKERS; GENOME, CHLOROPLAST; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RECOMBINASES; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; SERINE; TOBACCO; TRANSGENES;

EID: 84896403648     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/pbi.12139     Document Type: Article

References (37)

1. Abdeen, A., Schnell, J. and Miki, B. (2010) Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3. BMC Genomics, 11, 69.
2. Blechl, A., Lin, J., Shao, M., Thilmony, R. and Thomson, J. (2012) The Bxb1 recombinase mediates site-specific deletion in transgenic wheat. Plant Mol. Biol. Rep. 30, 1357-1366.
3. Broothaerts, W., Mitchell, H.J., Weir, B., Kaines, S., Smith, L.M.A., Yang, W., Mayer, J.E., Roa-Rodriguez, C. and Jefferson, R.A. (2005) Gene transfer to plants by diverse species of bacteria. Nature, 433, 629-633.
4. Coppoolse, E., de Vroomen, M., Roelofs, D., Smit, J., van Gennip, F., Hersmus, B.M., Nijkamp, H.J. and van Haaren, M.J. (2003) Cre recombinase expression can result in phenotypic aberrations in plants. Plant Mol. Biol. 51, 263-279.
5. Corneille, S., Lutz, K., Svab, Z. and Maliga, P. (2001) Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system. Plant J. 27, 171-178.
6. Corneille, S., Lutz, K.A., Azhagiri, A.K. and Maliga, P. (2003) Identification of functional lox sites in the plastid genome. Plant J. 35, 753-762.
7. Day, A. and Goldschmidt-Clermont, M. (2011) The chloroplast transformation toolbox: selectable markers and marker removal. Plant Biotechnol. J. 9, 540-553.
8. Dröge, M., Pühler, A. and Selbitschka, W. (1998) Horizontal gene transfer as a biosafety issue: a natural phenomenon of public concern. J. Biotechnol. 64, 75-90.
9. Ghosh, P., Bibb, L. and Hatfull, G. (2008) Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading. Proc. Natl Acad. Sci. USA, 105, 3238-3243.
10. Guda, C., Lee, S.B. and Daniell, H. (2000) Stable expression of a biodegradable protein-based polymer in tobacco chloroplasts. Plant Cell Rep. 19, 257-262.
11. Hajdukiewicz, P.T.J., Gilbertson, L. and Staub, J.M. (2001) Multiple pathways for Cre/lox-mediated recombination in plastids. Plant J. 27, 161-170.
12. Horsch, R.B., Fry, J.E., Hoffman, N.L., Eichholtz, D., Rogers, S.G. and Fraley, R.T. (1985) A simple and general method for transferring genes into plants. Science, 227, 1229-1231.
13. Iamtham, S. and Day, A. (2000) Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat. Biotechnol. 18, 1172-1176.
14. Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987) GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6, 3901-3907.
15. Kapusi, E., Kempe, K., Rubtsova, M., Kumlehn, J. and Gils, M. (2012) phiC31 integrase-mediated site-specific recombination in barley. PLoS ONE, 7, e45353.
16. Khan, M.S. and Maliga, P. (1999) Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants. Nat. Biotechnol. 17, 910-915.
17. Khan, M.S., Khalid, A.M. and Malik, K.A. (2005) Phage phiC31 integrase: a new tool in plastid genome engineering. Trends Plant Sci. 10, 1-3.
18. Kim, A., Ghosh, P., Aaron, M., Bibb, L., Jain, S. and Hatfull, G. (2003) Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene. Mol. Microbiol. 50, 463-473.
19. Kittiwongwattana, C., Lutz, K., Clark, M. and Maliga, P. (2007) Plastid marker gene excision by the phiC31 phage site-specific recombinase. Plant Mol. Biol. 64, 137-143.
20. Klaus, S.M.J., Huang, F.-C., Golds, T.J. and Koop, H.-U. (2004) Generation of marker-free plastid transformants using a transiently cointegrated selection gene. Nat. Biotechnol. 22, 225-229.
21. Kumar, S., Hahn, F.M., Baidoo, E., Kahlon, T.S., Wood, D.F., McMahan, C.M., Cornish, K., Keasling, J.D., Daniell, H. and Whalen, M.C. (2012) Remodeling the isoprenoid pathway in tobacco by expressing the cytoplasmic mevalonate pathway in chloroplasts. Metab. Eng. 14, 19-28.
22. Lutz, K.A., Corneille, S., Azhagiri, A.K., Svab, Z. and Maliga, P. (2004) A novel approach to plastid transformation utilizes the phiC31 phage integrase. Plant J. 37, 906-913.
23. Mediavilla, J., Jain, S., Kriakov, J., Ford, M.E., Duda, R.L., Jacobs, W.R., Hendrix, R.W. and Hatfull, G.F. (2000) Genome organization and characterization of mycobacteriophage Bxb1. Mol. Microbiol. 38, 955-970.
24. Que, Q., Wang, H.-Y. and Jorgensen, R.A. (1998) Distinct patterns of pigment suppression are produced by allelic sense and antisense chalcone synthase transgenes in petunia flowers. Plant J. 13, 401-409.
25. Rockhold, D.R., Chang, S., Taylor, N., Allen, P.V., McCue, K.F. and Belknap, W.R. (2008) Structure of two Solanum bulbocastanum polyubiquitin genes and expression of their promoters in transgenic potatoes. Am. J. Potato Res. 85, 219-226.
26. Sambrook, J. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
27. Serino, G. and Maliga, P. (1997) A negative selection scheme based on the expression of cytosine deaminase in plastids. Plant J. 12, 697-701.
28. Srivastava, V. and Ow, D.W. (2003) Rare instances of Cre-mediated deletion product maintained in transgenic wheat. Plant Mol. Biol. 52, 661-668.
29. Staub, J.M. and Maliga, P. (1994) Extrachromosomal elements in tobacco plastids. Proc. Natl Acad. Sci. USA, 91, 7468-7472.
30. Staub, J.M. and Maliga, P. (1995) Marker rescue from the Nicotiana tabacum plastid genome using a plastid/Escherichia coli shuttle vector. Mol. Gen. Genet. 249, 37-42.
31. Thomson, J.G., Yau, Y.Y., Blanvillain, R., Chiniquy, D., Thilmony, R. and Ow, D.W. (2009) ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome. Transgenic Res. 18, 237-248.
32. Thomson, J.G., Chan, R., Thilmony, R., Yau, Y.Y. and Ow, D.W. (2010) PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome. BMC Biotechnol. 10, 17. doi: 10.1186/1472-6750-10-17.
33. Thomson, J., Chan, R., Smith, J., Thilmony, R., Yau, Y.-Y., Wang, Y. and Ow, D. (2012) The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis. BMC Biotechnol. 12, 9.
34. Tungsuchat, T., Kuroda, H., Narangajavana, J. and Maliga, P. (2006) Gene activation in plastids by the CRE site-specific recombinase. Plant Mol. Biol. 61, 711-718.
35. Verma, D., Samson, N.P., Koya, V. and Daniell, H. (2008) A protocol for expression of foreign genes in chloroplasts. Nat. Protoc. 3, 739-758.
36. Wang, Y., Yau, Y.-Y., Perkins-Balding, D. and Thomson, J. (2011) Recombinase technology: applications and possibilities. Plant Cell Rep. 30, 267-285.
37. Ye, G.-N., Colburn, S.M., Xu, C.W., Hajdukiewicz, P.T.J. and Staub, J.M. (2003) Persistence of unselected transgenic DNA during a plastid transformation and segregation approach to herbicide resistance. Plant Physiol. 133, 402-410.