Dynamic quantitative assays of phagosomal function

Current Protocols in Immunology

Volumn , Issue SUPPL.102, 2013, Pages

  Podinovskaia, Maria ^a   VanderVen, Brian C ^a   Yates, Robin M ^b   Glennie, Sarah ^c   Fullerton, Duncan ^d   Mwandumba, Henry C ^e   Russell, David G ^a  

^a Department of Obstetrics Gynecology   (United States)

^b UNIVERSITY OF CALGARY   (Canada)

^c LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

^d MID CHESHIRE HOSPITALS NHS FOUNDATION TRUST   (United Kingdom)

^e QUEEN ELIZABETH CENTRAL HOSPITAL   (Malawi)

Author keywords

Macrophage; Phagocytosis; Phagosome; Phagosome maturation

Indexed keywords

SUPEROXIDE;

ARTICLE; BIOASSAY; BONE MARROW; CELL FUNCTION; CELL FUSION; CONFOCAL MICROSCOPY; FLOW CYTOMETRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUOROGENIC ASSAY; LYSOSOME; MACROPHAGE; MEASUREMENT; NONHUMAN; PHAGOSOME; PRIORITY JOURNAL; PROTEIN DEGRADATION; QUANTITATIVE ASSAY; SPECTROFLUOROMETRY; STATISTICAL PARAMETERS;

EID: 84896378720     PISSN: 19343671     EISSN: 1934368X     Source Type: Journal    
DOI: 10.1002/0471142735.im1434s102     Document Type: Article

References (12)

1. Blander, J.M. and Medzhitov, R. 2004. Regulation of phagosome maturation by signals from tolllike receptors. Science 304:1014-1018.
2. Podinovskaia, M., Lee, W., Caldwell, S., and Russell, D.G. 2013. Infection of macrophages with Mycobacterium tuberculosis induces global modifications to phagosomal function. Cell Microbiol. 15:843-859.
3. Russell, D.G. andYates,R.M. 2007. Toll-like receptors and phagosome maturation. Nat. Immunol. 8:217-218.
4. Russell, D.G., Vanderven, B.C., Glennie, S., Mwandumba, H., and Heyderman, R.S. 2009. The macrophage marches on its phagosome: Dynamic assays of phagosome function. Nat. Rev. Immunol. 9:594-600.
5. Rybicka, J.M., Balce, D.R., Chaudhuri, S., Allan, E.R., and Yates, R.M. 2012. Phagosomal proteolysis in dendritic cells is modulated byNADPH oxidase in a pH-independent manner. EMBO J. 31:932-944.
6. VanderVen, B.C., Yates, R.M., and Russell, D.G. 2009. Intraphagosomal measurement of the magnitude and duration of the oxidative burst. Traffic 10:372-378.
7. VanderVen, B.C., Hermetter, A., Huang, A., Maxfield, F.R., Russell, D.G., and Yates, RM. 2010. Development of a novel, cell-based chemical screen to identify inhibitors of intraphagosomal lipolysis in macrophages. Cytometry A 77:751-760.
8. Yates, R.M. and Russell, D.G. 2005. Phagosome maturation proceeds independently of stimulation of toll-like receptors 2 and 4. Immunity 23:409-417.
9. Yates, R.M. and Russell, D.G. 2008. Real-time spectrofluorometric assays for the lumenal environment of the maturing phagosome. Methods Mol. Biol. 445:311-325.
10. Yates, R.M., Hermetter, A., and Russell, D.G. 2005. The kinetics of phagosome maturation as a function of phagosome/lysosome fusion and acquisition of hydrolytic activity. Traffic 6: 413-420.
11. Yates, R.M., Hermetter, A., Taylor, G.A., and Russell, D.G. 2007. Macrophage activation downregulates the degradative capacity of the phagosome. Traffic 8:241-250.
12. Yates, R.M., Hermetter, A., and Russell, D.G. 2009. Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities. Methods Mol. Biol. 531:157-171.