Easy-to-use rapid gene amplification method for direct detection of RNA and DNA viruses in sera and feces from various animals

Journal of Virological Methods

Volumn 201, Issue , 2014, Pages 31-37

  Segawa, Takao ^a   Kobayashi, Yuki ^a   Sase, Yukina ^a   Itou, Takuya ^a   Suzuki, Miwa ^a   Endoh, Tomoko ^b   Nakanishi, Teruyuki ^a   Sakai, Takeo ^a  

^a NIHON UNIVERSITY   (Japan)

^b Shinagawa Aquarium   (Japan)

Author keywords

Direct detection; Easy to use; LAMP; RNA and DNA viruses

Indexed keywords

ARTICLE; BLOOD ANALYSIS; CALICIVIRUS; CONTROLLED STUDY; DIAGNOSTIC KIT; DNA EXTRACTION; FECES ANALYSIS; LIMIT OF DETECTION; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARVOVIRUS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; RNA EXTRACTION; SENSITIVITY ANALYSIS; TEMPERATURE SENSITIVITY; VIRUS DETECTION; ANIMAL; EVALUATION STUDY; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; NUCLEIC ACID AMPLIFICATION; PROCEDURES; RNA VIRUS; SENSITIVITY AND SPECIFICITY; SERUM; STAINING; TIME; VETERINARY; VIROLOGY; VIRUS DISEASES;

DNA VIRUS; FLUORESCENT DYE; ORGANIC COMPOUND; SYBR GREEN I;

ANIMALS; DNA VIRUSES; FECES; FLUORESCENT DYES; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; ORGANIC CHEMICALS; RNA VIRUSES; SENSITIVITY AND SPECIFICITY; SERUM; STAINING AND LABELING; TIME FACTORS; VIRUS DISEASES;

EID: 84896140275     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2014.01.019     Document Type: Article

References (21)

1. Cho H.S., Park N.Y. Detection of canine distemper virus in blood samples by reverse transcription loop-mediated isothermal amplification. J. Vet. Med. B. Infect. Dis. Vet. Public. Health. 2005, 52:410-413.
2. En F.X., Wei X., Jian L., Qin C. Loop-mediated isothermal amplification establishment for detection of pseudorabies virus. J. Virol. Methods. 2008, 151:35-39.
3. Goto M., Honda E., Ogura A., Nomoto A., Hanaki K. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques. 2009, 46:167-172.
4. Jayawardena S., Cheung C.Y., Barr I., Chan K.H., Chen H., Guan Y., Peiris J.S., Poon L.L. Loop-mediated isothermal amplification for influenza A (H5N1) virus. Emerg. Infect. Dis. 2007, 13:899-901.
5. Kaneko H., Kawana T., Fukushima E., Suzutani T. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J. Biochem. Biophys. Methods. 2007, 70:499-501.
6. Lee C.S., Moon H.J., Yang J.S., Park S.J., Song D.S., Kang B.K., Park B.K. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs. J. Virol. Methods. 2007, 139:39-43.
7. Mahony J., Chong S., Bulir D., Ruyter A., Mwawasi K., Waltho D. Multiplex loop-mediated isothermal amplification (M-LAMP) assay for the detection of influenza A/H1, A/H3 and influenza B can provide a specimen-to-result diagnosis in 40min with single genome copy sensitivity. J. Clin. Virol. 2013, 58:127-131.
8. Mahony J., Chong S., Bulir D., Ruyter A., Mwawasi K., Waltho D. Development of a sensitive loop-mediated isothermal amplification assay that provides specimen-to-result diagnosis of respiratory syncytial virus infection in 30minutes. J. Clin. Microbiol. 2013, 51:2696-2701.
9. Mori Y., Kanda H., Notomi T. Loop-mediated isothermal amplification (LAMP): recent progress in research and development. J. Infect. Chemother. 2013, 19:404-411.
10. Mori Y., Nagamine K., Tomita N., Notomi T. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun. 2001, 289:150-154.
11. Mukhopadhyay H.K., Amsaveni S., Matta S.L., Antony P.X., Thanislass J., Pillai R.M. Development and evaluation of loop-mediated isothermal amplification assay for rapid and sensitive detection of canine parvovirus DNA directly in faecal specimens. Lett. Appl. Microbiol. 2012, 55:202-209.
12. Nemoto M., Ohta M., Tsujimura K., Bannai H., Yamanaka T., Kondo T., Matsumura T. Direct detection of equine herpesvirus type 1 DNA in nasal swabs by loop-mediated isothermal amplification (LAMP). J. Vet. Med. Sci. 2011, 73:1225-1227.
13. Nie K., Qi S.X., Zhang Y., Luo L., Xie Y., Yang M.J., Zhang Y., Li J., Shen H., Li Q., Ma X.J. Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens. PloS. One. 2012, 7(12):e52486. 10.1371/journal.pone.0052486.
14. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic. Acids. Res. 2000, 28:E64.
15. Parida M., Sannarangaiah S., Dash P.K., Rao P.V., Morita K. Loop mediated isothermal amplification (LAMP): a new generation of innovative gene amplification technique; perspectives in clinical diagnosis of infectious diseases. Rev. Med. Virol. 2008, 18:407-421.
16. Ruwaida A.S.A., Schlegel H.G. Removal of RNA by heat-treatment. Applied Microbiology and Biotechnology. 1976, 2:81-89.
17. Schunck B., Kraft W., Truyen U. A simple touch-down polymerase chain reaction for the detection of canine parvovirus and feline panleukopenia virus in feces. J. Virol. Methods. 1995, 55:427-433.
18. Sharma S., Dash P.K., Santhosh S.R., Shukla J., Parida M., Rao P.V. Development of a quantitative competitive reverse transcription polymerase chain reaction (QC-RT-PCR) for detection and quantitation of Chikungunya virus. Mol. Biotechnol. 2010, 45:49-55.
19. Shin Y.J., Cho K.O., Cho H.S., Kang S.K., Kim H.J., Kim Y.H., Park H.S., Park N.Y. Comparison of one-step RT-PCR and a nested PCR for the detection of canine distemper virus in clinical samples. Aust. Vet. J. 2004, 82:83-86.
20. Sun J., Najafzadeh M.J., Vicente V., Xi L.de, Hoog G.S. Rapid detection of pathogenic fungi using loop-mediated isothermal amplification, exemplified by fonsecaea agents of chromoblastomycosis. J. Microbiol. Methods. 2010, 80:19-24.
21. Thekisoe O.M., Bazie R.S., Coronel-Servian A.M., Sugimoto C., Kawazu S., Inoue N. Stability of loop-mediated isothermal amplification (LAMP) reagents and its amplification efficiency on crude trypanosome DNA templates. J. Vet. Med. Sci. 2009, 71:471-475.