Quantification analysis of dot blot assays for human immunodeficiency virus type 1 and 2 antibodies

Iranian Journal of Basic Medical Sciences

Volumn 10, Issue 2, 2007, Pages 132-138

  Ravanshad, M ^a   Sabahi, F ^a   Mahboudi, F ^b  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

Author keywords

Densitometric analysis; Dot blot assay; Human immunodeficiency virus

Indexed keywords

ANTIGEN P24; GLYCOPROTEIN 36; GLYCOPROTEIN GP 120; HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN; HUMAN IMMUNODEFICIENCY VIRUS VACCINE; IMMUNOGLOBULIN G; UNCLASSIFIED DRUG;

ANTIBODY BLOOD LEVEL; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ARTICLE; BIOMASS; CONTROLLED STUDY; DISEASE TRANSMISSION; DOT HYBRIDIZATION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS 2; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; IMMUNE STATUS; IMMUNIZATION; IMMUNOAFFINITY CHROMATOGRAPHY; PROTEIN EXPRESSION; REPRODUCIBILITY; SIGNAL NOISE RATIO; WESTERN BLOTTING;

EID: 84894560730     PISSN: 20083866     EISSN: 20083874     Source Type: Journal    
DOI: 10.22038/IJBMS.2007.5280     Document Type: Article

References (20)

1. Allain JP, Laurian Y, Paul DA, Verroust F, Leuther M, Gazengel C, et al. Longterm evaluation of HIV antigen and antibodies to p24 and gp4l in patients with hemophilia. N Engl J Med 1987; 317:1114-1121.
2. Chen J, Wang L, Chen JJ, Sahu GK, Tyring S, Ramsey K,et al.. Detection of antibodies to human immunodeficiency virus (HIV) that recognize conformational epitopes of glycoproteins 160 and 41 often allows for early diagnosis of HIV infection.J Infect Dis 2002; 186(3):321-31.
3. Kiptoo MK, Mpoke SS, Ng’ang’a ZW. New indirect immunofluorescence assay as a confirmatory test for human immunodeficiency virus type 1. East Afr Med J 2004; 81(5):222-5.
4. Consortium for Retrovirus Serology Standardization. Serologic diagnosis of human immunodeficiency virus infection by Western blot testing. J Am Med Assoc 1998; 260: 674-679.
5. Cooper DA, Gold J, Maclean P, Donovan B, Finlayson R, Barnes TG,et al. Acute AIDS retrovirus infection. Definition of a clinical illness associated with seroconversion. Lancet i 1985; 537-540.
6. Goudsmit J J, Lange M A, Paul D A, Dawson G J. Antigenemia and antibody titers to core and envelope antigens in AIDS, AIDS-related complex, and subclinical human immunodeficiency virus infection. J Infect Dis 1987; 155:558-560
7. Ravanshad M, Sabahi F, Mahboudi F, Kazemnejad A. Evaluation of a new dot blot assay for confirmation of human immunodeficiency virus type 1 and 2 infections using recombinant p24, gp41, gp120 and gp36 antigens. Saudi Med J 2006; 27(1):31-6.
8. Frosner G G, Erfle V, Mellert W, Hehlmann R. Diagnostic significance of quantitative determination of HIV antibody specific for envelope and core protein. Lancet i 1997; 159-160.
9. Hofbauer J M, Schulz T F, Hengster P, Larcher C, Zangerle R, Kofler H P,et al. Comparison of Western blot based on recombinant-derived p4l with conventional tests for serodiagnosis of human immunodeficiency virus infections. J Clin Microbiol 1988; 26:116-120.
10. Shatzman A, Rosenberg M. Expression, identification, and characterization of recombinant gene products in Escherichia coli. Methods Enzymol 1997; 152:661-673.
11. Lange J, Goudsmit J. Decline of antibody reactivity to HIV core protein secondary to increase production of HIV antigen.Lanceti 1997; 448-449.
12. Saville RD, Constantine NT, Cleghorn FR, Jack N, Bartholomew C, Edwards J,et al. Fourth-generation enzyme-linked immunosorbent assay for the simultaneous detection of human immunodeficiency virus antigen and antibody. J Clin Microbiol 2001; 39(7):2518-24.
13. Ranki A, Johansson E, Krohn K. Interpretation of antibodies reacting solely with human retroviral core proteins.N Engl J Med 1998; 318:448-449.
14. Schmidit G, Amiraian K, Frey H, Stevens RW, Berns DS. Analysis of Western blot assays for human immunodeficiency virus antibodies and correlation with clinical status. J Clin Microbiol 1997; 25: 1993-1998.
15. Mayer K H L A, Falk D A, Paul G J, Dawson A M, Stoddard J, McCusker S P, et al. Correlation of recombinant antigen and antibody to the human immunodeficiency virus (HIV) with subsequent clinical outcomes in a cohort of asymptomatic homosexual males. Am J Med 1997; 83:208-212.
16. Yerly S, Simon F, Perrin L. Early diagnosis of primary HIV infections: using a combined screening test (p24 antigen and anti-HIV antibodies. Schweiz Med Wochenschr 1999; 129(8):319-22.
17. Strathdee S A, Frank JW, McLaughlin J, Leblanc M, Major C, O’Shaughnessy M, et al.Quantitative measure of human immunodeficiency virus-specific antibodies predicts progression to AIDS. J Infect Dis 1995; 172: 1375-1379.
18. Ng VL, Chiang CS, Debouck C, McGrath MS, Grove TH, Mills J. Reliable confirmation of antibodies to human immunodeficiency virus type 1 (HIV-1) with an enzyme-linked immunoassay using recombinant antigens derived from the HIV-1 gag, pol, and env genes. J Clin Microbiol 1989; 27(5):977-82.
19. Lawoko A, Johansson B, Rabinayaran D, Pipkorn R, Blomberg J. Increased immunoglobulin G, but not M, binding to endogenous retroviral antigens in HIV-1 infected persons. J Med Virol 2000; 62(4):435-44.
20. Weiss S H, Goeert J J, Sarngadharan M G, Bodner A J. The AIDS Seroepidemiology Collaborative Working Group, R. C. Gallo, and W. A. Blattner. Screening test for HTLV-II (AIDS agent) antibodies. J Am Med Assoc 1995; 253:221-225.