New methods as alternative or corrective measures for the pitfalls and artifacts of reverse transcription and polymerase chain reactions (RT-PCR) in cloning chimeric or antisense-accompanied RNA

RNA Biology

Volumn 10, Issue 6, 2013, Pages 957-967

  Yuan, Chengfu ^a   Liu, Yongming ^b   Yang, Min ^c   Liao, D Joshua ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b GUILIN MEDICAL UNIVERSITY   (China)

^c CHENGDU MEDICAL COLLEGE   (China)

Author keywords

Antisense; cDNA cloning; cDNA protection assay; Chiermic RNA; Polymerase chain reaction; Reverse transcription; RNA protection assay

Indexed keywords

COMPLEMENTARY DNA; DEOXYRIBONUCLEASE I; MESSENGER RNA; RNA; COMPLEMENTARY RNA; PRIMER DNA;

ARTICLE; CHIMERA; DNA DETERMINATION; DNA PURIFICATION; DNA STRAND; HUMAN; MOLECULAR CLONING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; CELL CULTURE; GENETICS; HELA CELL LINE; MCF 7 CELL LINE; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

BASE SEQUENCE; CELLS, CULTURED; CLONING, MOLECULAR; DNA PRIMERS; DNA, COMPLEMENTARY; HELA CELLS; HUMANS; MCF-7 CELLS; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, ANTISENSE;

EID: 84894537242     PISSN: 15476286     EISSN: 15558584     Source Type: Journal    
DOI: 10.4161/rna.24570     Document Type: Article

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