Preparation of a micropatterned rigid-soft composite substrate for probing cellular rigidity sensing

Methods in Cell Biology

Volumn 121, Issue , 2014, Pages 3-15

  Wong, Stephanie ^a   Guo, Wei hui ^a   Hoffecker, Ian ^a   Wang, Yu li ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

Cell adhesion; Durotaxis; Mechanosensing; Micropatterning; Photolithography

Indexed keywords

ACRYLIC ACID RESIN; BAYSILON; BIOMATERIAL; DIMETICONE; EPOXIDE; POLYACRYLAMIDE GELS; POLYMER; SU 8 COMPOUND; SU-8 COMPOUND;

ANIMAL; ARTICLE; CELL ADHESION; CELL CULTURE; CHEMISTRY; CULTURE TECHNIQUE; DUROTAXIS; HYDROGEL; MECHANICAL STRESS; MECHANOSENSING; MICROPATTERNING; PHOTOLITHOGRAPHY; PHYSIOLOGY; SURFACE PROPERTY; TUMOR MICROENVIRONMENT;

CELL ADHESION; DUROTAXIS; MECHANOSENSING; MICROPATTERNING; PHOTOLITHOGRAPHY;

ACRYLIC RESINS; ANIMALS; BIOCOMPATIBLE MATERIALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CELLULAR MICROENVIRONMENT; COATED MATERIALS, BIOCOMPATIBLE; DIMETHYLPOLYSILOXANES; EPOXY COMPOUNDS; HYDROGELS; POLYMERS; STRESS, MECHANICAL; SURFACE PROPERTIES;

EID: 84894076554     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-800281-0.00001-4     Document Type: Chapter

References (27)

1. Chen C.S. Geometric control of cell life and death. Science 1997, 276:1425-1428.
2. Choi Y.S., Vincent L.G., Lee A.R., Kretchmer K.C., Chirasatitsin S., Dobke M.K., et al. The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices. Biomaterials 2012, 33:6943-6951.
3. Dike L.E., Chen C.S., Mrksich M., Tien J., Whitesides G.M., Ingber D.E. Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cellular and Developmental Biology-Animal 1999, 35:441-448.
4. Discher D.E., Janmey P., Wang Y.-L. Tissue cells feel and respond to the stiffness of their substrate. Science (New York, NY) 2005, 310:1139-1143.
5. Engler A.J., Sen S., Sweeney H.L., Discher D.E. Matrix elasticity directs stem cell lineage specification. Cell 2006, 126:677-689.
6. Frey M.T., Wang Y.-L. A photo-modulatable material for probing cellular responses to substrate rigidity. Soft Matter 2009, 5:1918-1924.
7. Ghassemi S., Meacci G., Liu S., Gondarenko A.A., Mathur A., Roca-Cusachs P., et al. Cells test substrate rigidity by local contractions on submicrometer pillars. Proceedings of the National Academy of Sciences 2012, 109:5328-5333.
8. Gray D.S., Tien J., Chen C.S. Repositioning of cells by mechanotaxis on surfaces with micropatterned Young's modulus. Journal of Biomedical Materials Research 2003, 66A:605-614.
9. Hoffecker I.T., Guo W., Wang Y. Assessing the spatial resolution of cellular rigidity sensing using a micropatterned hydrogel-photoresist composite. Lab on a Chip 2011, 11:3538-3544.
10. Kloxin A.M., Benton J.A., Anseth K.S. In situ elasticity modulation with dynamic substrates to direct cell phenotype. Biomaterials 2010, 31:1-8.
11. Lo C.M., Wang H.B., Dembo M., Wang Y.L. Cell movement is guided by the rigidity of the substrate. Biophysical Journal 2000, 79:144-152.
12. McBeath R., Pirone D.M., Nelson C.M., Bhadriraju K., Chen C.S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Developmental Cell 2004, 6:483-495.
13. Nemir S., Hayenga H.N., West J.L. PEGDA hydrogels with patterned elasticity: Novel tools for the study of cell response to substrate rigidity. Biotechnology and Bioengineering 2010, 105:636-644.
14. Pelham R.J., Wang Y. Cell locomotion and focal adhesions are regulated by substrate flexibility. Cell 1997, 94:13661-13665.
15. Pouthas F., Girard P., Lecaudey V., Ly T.B.N., Gilmour D., Boulin C., et al. In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum. Journal of Cell Science 2008, 121:2406-2414.
16. Singhvi R., Kumar A., Lopez G.P., Stephanopoulos G.N., Wang D.I., Whitesides G.M., et al. Engineering cell shape and function. Science (New York, NY) 1994, 264:696-698.
17. Sun Y., Jiang L.-T., Okada R., Fu J. UV-modulated substrate rigidity for multiscale study of mechanoresponsive cellular behaviors. Langmuir: The ACS Journal of Surfaces and Colloids 2012, 28:10789-10796.
18. Tan J.L., Tien J., Pirone D.M., Gray D.S., Bhadriraju K., Chen C.S. Cells lying on a bed of microneedles: An approach to isolate mechanical force. PNAS 2002, 100:1484-1489.
19. Théry M., Piel M. Adhesive micropatterns for cells: A microcontact printing protocol. Cold Spring Harbor protocols 2009, 4(7):888-898.
20. Trichet L., Le Digabel J., Hawkins R.J., Vedula S.R.K., Gupta M., Ribrault C., et al. Evidence of a large-scale mechanosensing mechanism for cellular adaptation to substrate stiffness. Proceedings of the National Academy of Sciences 2012, 109:6933-6938.
21. Tsai I.Y., Crosby A.J., Russell T.P. Surface patterning. Methods in cell biology: Cell mechanics 2007, 67-87. Academic Press, San Diego, CA. Y.L. Wang, D.E. Discher (Eds.).
22. Tseng Q., Wang I., Duchemin-Pelletier E., Azioune A., Carpi N., Gao J., et al. A new micropatterning method of soft substrates reveals that different tumorigenic signals can promote or reduce cell contraction levels. Lab on a Chip 2011, 11:2231-2240.
23. Voskerician G., Shive M.S., Shawgo R.S., von Recum H., Anderson J.M., Cima M.J., et al. Biocompatibility and biofouling of MEMS drug delivery devices. Biomaterials 2003, 24:1959-1967.
24. Wang H.B., Dembo M., Hanks S.K., Wang Y. Focal adhesion kinase is involved in mechanosensing during fibroblast migration. PNAS 2001, 98:11295-11300.
25. Wang N., Ostuni E., Whitesides G.M., Ingber D.E. Micropatterning tractional forces in living cells. Cell Motility and the Cytoskeleton 2002, 52:97-106.
26. Whitesides G.M., Ostuni E., Takayama S., Jiang X., Ingber D.E. Soft lithography in biology and biochemistry. Annual Review of Biomedical Engineering 2001, 3:335-373.
27. Wong J.Y., Velasco A., Rajagopalan P., Pham Q. Directed movement of vascular smooth muscle cells on gradient-compliant hydrogels. Langmuir 2003, 19:1908-1913.